Impact of cerium oxide nanoparticles shape on their in vitro cellular toxicity

Abstract Background and aims Cerium oxide nanoparticles are effective scavengers of reactive oxygen species and have been proposed as a treatment for oxidative stress-related diseases. Consequently, we aimed to investigate the effect of these nanoparticles on hepatic regeneration after liver injury by partial hepatectomy and acetaminophen overdose. Methods All the in vitro experiments were performed in HepG2 cells. For the acetaminophen and partial hepatectomy experimental models, male Wistar rats were divided into three groups: (1) nanoparticles group, which received 0.1 mg/kg cerium nanoparticles i.v. twice a week for 2 weeks before 1 g/kg acetaminophen treatment, (2) N-acetyl-cysteine group, which received 300 mg/kg of N-acetyl-cysteine i.p. 1 h after APAP treatment and (3) partial hepatectomy group, which received the same nanoparticles treatment before partial hepatectomy. Each group was matched with vehicle-controlled rats. Results In the partial hepatectomy model, rats treated with cerium oxide nanoparticles showed a significant increase in liver regeneration, compared with control rats. In the acetaminophen experimental model, nanoparticles and N-acetyl-cysteine treatments decreased early liver damage in hepatic tissue. However, only the effect of cerium oxide nanoparticles was associated with a significant increment in hepatocellular proliferation. This treatment also reduced stress markers and increased cell cycle progression in hepatocytes and the activation of the transcription factor NF-κB in vitro and in vivo. Conclusions Our results demonstrate that the nanomaterial cerium oxide, besides their known antioxidant capacities, can enhance hepatocellular proliferation in experimental models of liver regeneration and drug-induced hepatotoxicity.

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In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates

Nanomaterials ◽

10.3390/nano9111565 ◽

2019 ◽

Vol 9 (11) ◽

pp. 1565 ◽

Cited By ~ 7

Author(s):

Turin-Moleavin ◽

Fifere ◽

Lungoci ◽

Rosca ◽

Coroaba ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Iron Oxide ◽

Cerium Oxide ◽

Chemical Activation ◽

Radical Scavenging ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications.

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Cerium Oxide Nanoparticles as Novel Tool in Glioma Treatment: An In vitro Study

Journal of Nanomedicine & Nanotechnology ◽

10.4172/2157-7439.1000474 ◽

2017 ◽

Vol 08 (06) ◽

Cited By ~ 3

Author(s):

Sack Zschauer M ◽

Bader S ◽

Brenneisen P

Keyword(s):

Cerium Oxide ◽

In Vitro Study ◽

Vitro Study ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

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In-vitro interaction of cerium oxide nanoparticles with hemoglobin, insulin, and dsDNA at 310.15 K: Physicochemical, spectroscopic and in-silico study

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2020.03.067 ◽

2020 ◽

Vol 156 ◽

pp. 1022-1044 ◽

Cited By ~ 1

Author(s):

Rajlakshmi Chetty ◽

Man Singh

Keyword(s):

Cerium Oxide ◽

In Silico ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

In Silico Study ◽

In Vitro Interaction

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Cerium oxide nanoparticles promote HSA fibrillation in vitro

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2017.05.180 ◽

2017 ◽

Vol 103 ◽

pp. 1138-1145 ◽

Cited By ~ 3

Author(s):

Gajalakshmi Sekar ◽

N.Prem Kumar ◽

Amitava Mukherjee ◽

Natarajan Chandrasekaran

Keyword(s):

Cerium Oxide ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles

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Effects of Cerium Oxide Nanoparticles on the Proliferation, Differentiation, and Mineralization Function of Primary Osteoblasts In Vitro

Biological Trace Element Research ◽

10.1007/s12011-013-9655-2 ◽

2013 ◽

Vol 153 (1-3) ◽

pp. 411-418 ◽

Cited By ~ 13

Author(s):

Guoqiang Zhou ◽

Guangqi Gu ◽

Yang Li ◽

Qun Zhang ◽

Wenying Wang ◽

...

Keyword(s):

Cerium Oxide ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Primary Osteoblasts

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A Complete In Vitro Toxicological Assessment of the Biological Effects of Cerium Oxide Nanoparticles: From Acute Toxicity to Multi-Dose Subchronic Cytotoxicity Study

Nanomaterials ◽

10.3390/nano11061577 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1577

Author(s):

Adrián García-Salvador ◽

Alberto Katsumiti ◽

Elena Rojas ◽

Carol Aristimuño ◽

Mónica Betanzos ◽

...

Keyword(s):

Acute Toxicity ◽

Cerium Oxide ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Interstitial Tissue ◽

Airway Epithelial ◽

Toxicological Assessment ◽

Human Airway ◽

Toxicological Studies

Engineered nanomaterials (ENMs) are of significant relevance due to their unique properties, which have been exploited for widespread applications. Cerium oxide nanoparticles (CeO2-NPs) are one of most exploited ENM in the industry due to their excellent catalytic and multi-enzyme mimetic properties. Thus, the toxicological effects of these ENMs should be further studied. In this study, the acute and subchronic toxicity of CeO2-NPs were assessed. First, an in vitro multi-dose short-term (24 h) toxicological assessment was performed in three different cell lines: A549 and Calu3 were used to represented lung tissue and 3T3 was used as an interstitial tissue model. After that, a sub-chronic toxicity assessment (90 days) of these NPs was carried out on a realistic and well-established reconstituted primary human airway epithelial model (MucilAir™), cultured at the Air–Liquid Interface (ALI), to study the long-term effects of these particles. Results showed minor toxicity of CeO2-NPs in acute exposures. However, in subchronic exposures, cytotoxic and inflammatory responses were observed in the human airway epithelial model after 60 days of exposure to CeO2-NPs. These results suggest that acute toxicity approaches may underestimate the toxicological effect of some ENMs, highlighting the need for subchronic toxicological studies in order to accurately assess the toxicity of ENM and their cumulative effects in organisms.

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Detrimental effects of cerium oxide nanoparticles on testis, sperm parameters quality, and in vitro fertilization in mice: An experimental study

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v19i9.9712 ◽

2021 ◽

pp. 801-810

Author(s):

Elnaz Hosseinalipour ◽

Mojtaba Karimipour ◽

Abbas Ahmadi

Keyword(s):

In Vitro Fertilization ◽

Cerium Oxide ◽

Reproductive System ◽

Male Reproductive System ◽

Sperm Parameters ◽

Sperm Chromatin ◽

Cerium Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Vitro Fertilization

Background: Cerium oxide nanoparticles (CeO2 NPs) as an important nanomaterial have a wide range of applications in many fields and human beings’ exposure to this nanomaterial is unavoidable. The effects of CeO2 NPs on the male reproductive system are controversial. Objective: To determine the effects of the administration of CeO2 NPs on the testis tissue, sperm parameters, and in vitro fertilization (IVF) in mice. Materials and Methods: Twenty-four male mice were divided into three groups (n = 8/each): one control and two experimental groups receiving CeO2 NPs at doses of 50 and 100 mg/kg body weight, respectively, for 35 days. At the end of the experiment, the diameter of seminiferous tubules (SNTs), epithelial height of SNTs, spermiogenesis index in testes, sperm parameters (count, motility, viability, and morphology), sperm chromatin condensation, DNA integrity, and IVF assays were analyzed. Results: Histological results showed that the tubular diameter, the epithelial height of the SNTs, and the spermiogenesis index were significantly decreased in the experimental groups receiving CeO2 NPs. All sperm parameters in the experimental groups were significantly reduced and, additionally, the percentages of immature sperms and sperms with DNA damage were significantly increased in groups treated with CeO2 NPs compared to the control. Furthermore, the rates of IVF and in vitro embryo development were decreased. Conclusion: Collectively, the current study showed that oral administration of CeO2 NPs in mice had detrimental effects on the male reproductive system through inducing testicular tissue alterations, decreasing sperm parameters quality, and also diminishing the IVF rate and in vitro embryonic development. Key words: Cerium oxide, Testis, Sperm, Fertilization, Mice.

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