The influence of cigarette smoking on human sperm quality and DNA fragmentation

Toxicology ◽  
2006 ◽  
Vol 223 (1-2) ◽  
pp. 54-60 ◽  
Author(s):  
Sandrine Sepaniak ◽  
Thierry Forges ◽  
Hubert Gerard ◽  
Bernard Foliguet ◽  
Marie-Christine Bene ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Human Sperm

scholarly journals Effects of human sperm cryopreservation on apoptotic markers in normozoospermic and non-normozoospermic patients

Zygote ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 308-313 ◽  
Author(s):  
Seda Karabulut ◽  
Asuman Demiroğlu-Zergeroğlu ◽  
Elif Yılmaz ◽  
Pelin Kutlu ◽  
İlknur Keskin
Keyword(s):  
Dna Fragmentation ◽  
Caspase 3 ◽  
Sperm Quality ◽  
Human Sperm ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Sperm Cryopreservation ◽  
Negative Effects ◽  
Apoptotic Markers ◽  
Neat Semen

SummaryThe negative effects of cryopreservation on sperm parameters are well documented but little information is known about molecular basis of the process. The aim of the present study was to investigate the possible effects of sperm cryopreservation on main apoptotic signs including DNA fragmentation and caspase-3 activation and to determine if these effects vary according to sperm parameters. Sperm samples of 72 patients were cryopreserved. The patients were sub-grouped as normozoospermic or non-normozoospermic patients according to their semen parameters. DNA fragmentation rates and caspase-3 activation levels were analyzed before and after cryopreservation in both groups. Mean DNA fragmentation rate was increased significantly from 23.98% in neat semen samples to 27.34% after cryopreservation (P = 0.03). DNA fragmentation rates were slightly higher in non-normozoospermic patients compared with the normozoospermic patients in both the neat semen and after cryopreservation (23.25 and 24.71% vs. 26.32 and 28.36%, respectively) although the difference obtained were not statistically significant. An increasing trend for caspase-3 activations (0.093 vs. 0.116) was observed after cryopreservation but the differences were not statistically significant. Caspase-3 activation was found to be slightly higher in non-normozoospermic patients both in the neat semen and after cryopreservation compared with the normozoospermic patients but the differences were not statistically significant. Caspase-3 expression was also shown using immunocytochemistry in both fresh ejaculated sperm and thawed sperm after cryopreservation but at different localizations. The cryopreservation process had detrimental effects on sperm quality but the quality of the sperm samples was not adversely effective for the apoptotic markers including DNA fragmentation and caspase-3 activation patterns. In fact, it was the cryopreservation process itself that adversely effected the above apoptotic markers and apoptosis. It was concluded therefore that sperm cell cryopreservation triggers apoptosis after thawing and this process adversely affects semen parameters.

scholarly journals The influence of ginger (Zingiber officinale) on human sperm quality and DNA fragmentation: A double-blind randomized clinical trial

2016 ◽  
Vol 14 (8) ◽  
pp. 533-540 ◽  
Author(s):  
Jalil Hosseini ◽  
Azar Mardi Mamaghani ◽  
Hani Hosseinifar ◽  
Mohammad Ali Sadighi Gilani ◽  
Farid Dadkhah ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Randomized Clinical Trial ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Zingiber Officinale ◽  
Human Sperm ◽  
Double Blind

Application of erythrocyte lysing buffer (ELB) has detrimental effects on human sperm quality parameters, DNA fragmentation and chromatin structure

Andrologia ◽  
2020 ◽  
Vol 52 (9) ◽  
Author(s):  
Fatemeh Yazdinejad ◽  
Leila Heydari ◽  
Leila Motamed zadeh ◽  
Seyed Morteza Seifati ◽  
Azam Agha‐Rahimi
Keyword(s):  
Dna Fragmentation ◽  
Chromatin Structure ◽  
Sperm Quality ◽  
Human Sperm ◽  
Quality Parameters

scholarly journals Cigarette smoking and human sperm quality assessed by laser-Doppler spectroscopy and DNA flow cytometry

Reproduction ◽  
1989 ◽  
Vol 86 (2) ◽  
pp. 731-736 ◽  
Author(s):  
N. B. Oldereid ◽  
H. Rui ◽  
O. P. F. Clausen ◽  
K. Purvis
Keyword(s):  
Flow Cytometry ◽  
Cigarette Smoking ◽  
Sperm Quality ◽  
Human Sperm ◽  
Laser Doppler ◽  
Dna Flow Cytometry ◽  
Doppler Spectroscopy

scholarly journals Melatonin and Caffeine Supplementation Used, Respectively, as Protective and Stimulating Agents in the Cryopreservation of Human Sperm Improves Survival, Viability, and Motility after Thawing compared to Traditional TEST-Yolk Buffer

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Juliana R. Pariz ◽  
Caroline Ranéa ◽  
Rosa A. C. Monteiro ◽  
Donald P. Evenson ◽  
Joël R. Drevet ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Human Sperm ◽  
Mitochondrial Activity ◽  
Ros Production ◽  
Stimulant Effect ◽  
Progressive Motility ◽  
Before And After

Cryopreservation processes can damage spermatozoa and impair structural and functional cell characteristics. Plasma, nuclear membranes, and cellular organelles can suffer from the freeze and thaw process. This study evaluates the protective and stimulant effect of melatonin and caffeine supplementation on the functional characteristics of human spermatozoa before and after freezing. Thirty seminal samples from normozoospermic men aged 19–45 years old collected between October 2012 and May 2017 were included. Semen samples were supplemented with either 2 mM melatonin (MEL) prior to cryopreservation, 2 mM caffeine (CAF) in postthaw, or CAF and MEL (CM) in precryopreservation and postthaw, respectively. Kinetics and seminal parameters, mitochondrial activity, DNA fragmentation, and reactive oxygen species (ROS) levels were analyzed before and after cryopreservation. A significant reduction in sperm concentration, total and progressive motility, sperm kinetics, and mitochondrial activity, as well as a significant increase in DNA fragmentation and ROS production in postthaw samples compared to fresh samples, was identified. After administration of a caffeine and/or melatonin supplement, there was a significant increase in progressive motility in the CAF (p=0.005) and CM (p=0.048) groups, as well as mitochondrial activity in the CM group (p<0.05). Cryopreservation has negative effects on overall sperm quality and increases ROS production. A combination of caffeine and melatonin in prefreeze and postthaw sperm samples has proven to be a very effective and simple way to improve semen quality. This will be particularly useful for initial low-quality semen samples, those which suffer the most from the freezing/thawing process.

scholarly journals Antioxidant Activity of Opuntia SP. Fruit Extracts on Human Sperm Quality and Cryopreservation Cycle

2021 ◽  
Author(s):  
Martina Contino ◽  
Elena Maria Scalisi ◽  
Roberta Pecoraro ◽  
Chiara Failla ◽  
Sara Ignoto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Fragmentation ◽  
Acrosome Reaction ◽  
Sperm Quality ◽  
Human Sperm ◽  
Semen Parameters ◽  
Fruit Extracts ◽  
Freezing Medium ◽  
Before And After ◽  
And Oxidative Stress

Opuntia sp. contain antioxidant phytochemicals resistant to ROS damage, whose excess negatively affect fertilization. We investigate the activity of fruit extracts of O. dillenii and O. ficus indica (cv red and yellow) on sperm quality and cryopreservation. In the first experiment, we exposed the samples to extracts (50 &micro;l) for 1 hour to then evaluate semen parameters (vitality, motility, acrosome reaction, DNA fragmentation and oxidative stress). The results showed a significant increase in the motility (86%&plusmn;0.19 for OFI cv yellow, 82%&plusmn;0.15 for OFI cv red and 90%&plusmn;0.08 for O. dillenii) compared to the control (80%&plusmn;0.17). Moreover, we noted a reduction of DNA fragmentation on treated (3%&plusmn;0.03 in OFI cv yellow, 7%&plusmn;0.09 in OFI cv red and 5%&plusmn;0.07 in O. dillenii) than the control (40%&plusmn;0.14). Furthermore, the oxidative stress was reduced after exposure to solutions (3.15mV in the control and 2.94mV in the treated). In the second experiment, 50 &micro;l of solutions were added to the Freezing medium. After thawing, we observed an improvement in vitality and the number of intact acrosomes. Our results suggest that Opuntia sp. fruit extracts improve sperm quality, both before and after cryopreservation, optimizing the potential of fertilization of sperm cells.

Use of laptop computers connected to internet through Wi-Fi decreases human sperm motility and increases sperm DNA fragmentation

2012 ◽  
Vol 97 (1) ◽  
pp. 39-45.e2 ◽  
Author(s):  
Conrado Avendaño ◽  
Ariela Mata ◽  
César A. Sanchez Sarmiento ◽  
Gustavo F. Doncel
Keyword(s):  
Dna Fragmentation ◽  
Sperm Motility ◽  
Human Sperm ◽  
Sperm Dna Fragmentation ◽  
Laptop Computers ◽  
Sperm Dna

scholarly journals Potential effect of tobacco cigarettes smoking on global DNA methylation status and protamines transcripts in human spermatozoa

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Mohammed M. Laqqan ◽  
Maged M. Yassin
Keyword(s):  
Dna Methylation ◽  
Cigarette Smoking ◽  
Dna Fragmentation ◽  
Methylation Status ◽  
Human Spermatozoa ◽  
Global Dna Methylation ◽  
Transcription Level ◽  
Heavy Smokers ◽  
Protamine 1 ◽  
Protamine 2

Abstract Background Epigenetics refers to an alteration in gene expression without alteration in the sequence of DNA and this process may be affected by environmental factors and lifestyle like cigarette smoking. This study was designed to evaluate the potential effect of cigarette smoking on the global DNA methylation status and the transcription level of protamine 1 and protamine 2 in human spermatozoa. A total of 188 semen samples were collected from men with a mean age of 34.9 ± 5.8 years old (98 heavy smokers and 90 non-smokers). The DNA and RNA were isolated from purified spermatozoa, then the status of global DNA methylation and the transcription level of protamine 1 and protamine 2 were evaluated using ELISA and qPCR, respectively. The chromatin non-condensation and DNA fragmentation in human spermatozoa were evaluated using chromomycin A3 staining and TUNEL assay, respectively. Results A significant increase has been found in the status of global DNA methylation in spermatozoa of heavy smokers compared to non-smokers (7.69 ± 0.69 ng/μl vs. 4.90 ± 0.40 ng/μl, P < 0.001). Additionally, a significant reduction has been found in transcription level of protamine 1 (25.49 ± 0.31 vs. 23.94 ± 0.40, P < 0.001) and protamine 2 (28.27 ± 0.39 vs. 23.45 ± 0.30, P < 0.001) in heavy smokers. A downregulation has been found in the transcription level of protamine 1 and protamine 2 with a fold change of 0.497 and 0.047, respectively. A significant increase has been shown in the level of DNA fragmentation and chromatin non-condensation in heavy smokers compared to non-smokers (P < 0.001). On the other hand, a significant positive correlation has been found between sperm chromatin non-condensation, sperm DNA fragmentation, transcription level of protamine 1, transcription level of protamine 2, and global DNA methylation status (r = 0.304, P < 0.001; r = 0.399, P < 0.001; r = 0.216, P = 0.003; r = 0.494, P < 0.001, respectively). Conclusion Tobacco cigarette smoking has a potential influence on the global DNA methylation and the transcription level of protamine genes in human spermatozoa, and consequently, affect negatively on the semen parameters.

Sign in / Sign up

Export Citation Format

Share Document