scholarly journals Effects of human sperm cryopreservation on apoptotic markers in normozoospermic and non-normozoospermic patients

Zygote ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 308-313 ◽  
Author(s):  
Seda Karabulut ◽  
Asuman Demiroğlu-Zergeroğlu ◽  
Elif Yılmaz ◽  
Pelin Kutlu ◽  
İlknur Keskin
Keyword(s):  
Dna Fragmentation ◽  
Caspase 3 ◽  
Sperm Quality ◽  
Human Sperm ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Sperm Cryopreservation ◽  
Negative Effects ◽  
Apoptotic Markers ◽  
Neat Semen

SummaryThe negative effects of cryopreservation on sperm parameters are well documented but little information is known about molecular basis of the process. The aim of the present study was to investigate the possible effects of sperm cryopreservation on main apoptotic signs including DNA fragmentation and caspase-3 activation and to determine if these effects vary according to sperm parameters. Sperm samples of 72 patients were cryopreserved. The patients were sub-grouped as normozoospermic or non-normozoospermic patients according to their semen parameters. DNA fragmentation rates and caspase-3 activation levels were analyzed before and after cryopreservation in both groups. Mean DNA fragmentation rate was increased significantly from 23.98% in neat semen samples to 27.34% after cryopreservation (P = 0.03). DNA fragmentation rates were slightly higher in non-normozoospermic patients compared with the normozoospermic patients in both the neat semen and after cryopreservation (23.25 and 24.71% vs. 26.32 and 28.36%, respectively) although the difference obtained were not statistically significant. An increasing trend for caspase-3 activations (0.093 vs. 0.116) was observed after cryopreservation but the differences were not statistically significant. Caspase-3 activation was found to be slightly higher in non-normozoospermic patients both in the neat semen and after cryopreservation compared with the normozoospermic patients but the differences were not statistically significant. Caspase-3 expression was also shown using immunocytochemistry in both fresh ejaculated sperm and thawed sperm after cryopreservation but at different localizations. The cryopreservation process had detrimental effects on sperm quality but the quality of the sperm samples was not adversely effective for the apoptotic markers including DNA fragmentation and caspase-3 activation patterns. In fact, it was the cryopreservation process itself that adversely effected the above apoptotic markers and apoptosis. It was concluded therefore that sperm cell cryopreservation triggers apoptosis after thawing and this process adversely affects semen parameters.

scholarly journals Antioxidant Activity of Opuntia SP. Fruit Extracts on Human Sperm Quality and Cryopreservation Cycle

2021 ◽  
Author(s):  
Martina Contino ◽  
Elena Maria Scalisi ◽  
Roberta Pecoraro ◽  
Chiara Failla ◽  
Sara Ignoto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Fragmentation ◽  
Acrosome Reaction ◽  
Sperm Quality ◽  
Human Sperm ◽  
Semen Parameters ◽  
Fruit Extracts ◽  
Freezing Medium ◽  
Before And After ◽  
And Oxidative Stress

Opuntia sp. contain antioxidant phytochemicals resistant to ROS damage, whose excess negatively affect fertilization. We investigate the activity of fruit extracts of O. dillenii and O. ficus indica (cv red and yellow) on sperm quality and cryopreservation. In the first experiment, we exposed the samples to extracts (50 µl) for 1 hour to then evaluate semen parameters (vitality, motility, acrosome reaction, DNA fragmentation and oxidative stress). The results showed a significant increase in the motility (86%±0.19 for OFI cv yellow, 82%±0.15 for OFI cv red and 90%±0.08 for O. dillenii) compared to the control (80%±0.17). Moreover, we noted a reduction of DNA fragmentation on treated (3%±0.03 in OFI cv yellow, 7%±0.09 in OFI cv red and 5%±0.07 in O. dillenii) than the control (40%±0.14). Furthermore, the oxidative stress was reduced after exposure to solutions (3.15mV in the control and 2.94mV in the treated). In the second experiment, 50 µl of solutions were added to the Freezing medium. After thawing, we observed an improvement in vitality and the number of intact acrosomes. Our results suggest that Opuntia sp. fruit extracts improve sperm quality, both before and after cryopreservation, optimizing the potential of fertilization of sperm cells.

scholarly journals Low amounts of mitochondrial reactive oxygen species define human sperm quality

Reproduction ◽  
2014 ◽  
Vol 147 (6) ◽  
pp. 817-824 ◽  
Author(s):  
Mónica Marques ◽  
Ana Paula Sousa ◽  
Artur Paiva ◽  
Teresa Almeida-Santos ◽  
João Ramalho-Santos
Keyword(s):  
Reactive Oxygen Species ◽  
Assisted Reproduction ◽  
Density Gradient Centrifugation ◽  
Sperm Quality ◽  
Gradient Centrifugation ◽  
Reactive Oxygen ◽  
Human Sperm ◽  
Semen Parameters ◽  
Oxygen Species ◽  
Male Gametes

We have applied the mitochondria-specific superoxide fluorescent probe MitoSOX Red (MitoSOX) to detect mitochondria-specific reactive oxygen species (mROS) production in human sperm samples using flow cytometry. We show that human ejaculates are heterogeneous in terms of mROS production, with three subpopulations clearly detectable, comprising sperm that produce increasing amounts of mROS (MitoSOX−, MitoSOX+, and MitoSOX++). The sperm subpopulation producing the lowest amount of mROS represented the most functional subset of male gametes within the ejaculate, as it was correlated with the highest amount of live and non-apoptotic sperm and increased both in samples with better semen parameters and in samples processed by both density-gradient centrifugation and swim-up, both known to select for higher quality sperm. Importantly, the MitoSOX− subpopulation was clearly more prevalent in samples that gave rise to pregnancies following assisted reproduction. Our work, therefore, not only describe discreet human sperm heterogeneity at the mROS level but also suggests that mROS may represent a strategy to both evaluate sperm samples and isolate the most functional gametes for assisted reproduction.Free Portuguese abstractA Portuguese translation of this abstract is freely available athttp://www.reproduction-online.org/content/147/6/817/suppl/DC1

scholarly journals Male cancer patient sperm cryopreservation for fertility preservation: 10-year monocentric experience

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Xiao Liu ◽  
Bo Liu ◽  
Shasha Liu ◽  
Yang Xian ◽  
Wenrui Zhao ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cancer Patients ◽  
Semen Quality ◽  
Sperm Quality ◽  
Semen Parameters ◽  
Germ Cell Tumours ◽  
Sperm Cryopreservation ◽  
Sperm Banking ◽  
Male Cancer Patient ◽  
Chinese Cancer Patients

Abstract Background Sperm cryopreservation, an effective method for preserving male fertility, is very advantageous for men suffering from cancer. Unfortunately, as both physicians and cancer patients are unaware of the possibilities for sperm cryopreservation, the data on evaluation of semen parameters and disposition of cryopreserved samples among Chinese cancer patients are scarce. Results Male tumours were classified into six major types, germ cell tumours (26 %), haematological neoplasms (28 %), head and neck cancers (19 %), thoracic tumours (4 %), abdominal tumours (10 %), and others (13 %). Haematological neoplasm was the most prevalent cancer among our cohort of patients who opted for sperm banking, followed by germ cell tumours. Patients with germ cell tumours had the lowest pre-thaw and post-thaw seminal sperm concentrations. We separately compared patients with testicular tumours, lymphoma, and leukaemia, and found that leukaemia patients had the lowest pre-thaw sperm concentrations. Most cancer patients (58 %) chose to keep their specimens stored, while 31 % chose to discard the specimens. Over the years, only 13 patients (4 %) returned to use their spermatozoa by assisted reproductive technology. Of the stored samples, patients with germ cell tumours constituted the highest proportion (29.3 %). Moreover, the percentage of haematological neoplasm patients who had no spermatozoa frozen was the highest (46.2 %). Conclusions The present data confirm the deleterious impact of various cancers on semen quality. Leukaemia was associated with the worst semen quality and the highest number of semen samples that could not be frozen. We suggest that sperm quality may have decreased even before anti-neoplastic treatment and that sperm banking before treatment should be strongly recommended for cancer patients. A sperm banking programme before gonadotoxic therapy requires close cooperation between assisted reproduction centres and cancer clinics.

scholarly journals Sperm cryopreservation of male cancer patients for  fertility preservation : 10-year monocentric experience

2021 ◽  
Author(s):  
Xiao Liu ◽  
Bo Liu ◽  
shasha liu ◽  
yang xian ◽  
wenrui zhao ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cancer Patients ◽  
Fertility Preservation ◽  
Sperm Quality ◽  
Germ Cell Tumors ◽  
Semen Parameters ◽  
Sperm Cryopreservation ◽  
Sperm Banking ◽  
Hematological Neoplasms ◽  
Body Regions

Abstract Background: Semen cryopreservation is an effective method to preserve fertility, which is very important for male cancer patients. Unfortunately, due to unaware of the opportunities for sperm cryopreservation for both physicians and cancer patients, not a lot of data on evaluating the semen parameters and dispositions of the cryopreserved samples of Chinese cancer population are available in the literature. Methods: We retrospectively reviewed semen parameters as well as the clinical outcomes of assisted reproductive treatments (ART) of 339 male cancer patients of Chinese population who were referred to our center from 2010 to 2019 for fertility preservation. Results: We first classify the male tumors into six major types according to body regions. The most prevalent cancer patients who came from our cohort for sperm banking were hematological neoplasms patients, and the second cancers were germ cell tumors. Patients with germ cell tumors had the lowest pre- thaw and post-thaw concentration among the six major cancer types. However, we separately compared among testicular tumors, lymphoma and leukemia, it turned out that leukemia had the lowest pre-thaw concentration. Most cancer patients (58%) chose to go on keeping their specimens in storage. The second proportion selected to discard their specimens electively (31%). Over the years, there were only 13 patients (4%) returned to use their sperm by ART. In the storage samples, germ cell tumors were the most proportion ones (29.3%). Moreover, in the unfrozen samples, the percentage of hematological neoplasms were the most (45.5%).Conclusions: To our knowledge, we had owned the most numbers of male cancers who came to sperm bank for fertility conservation in the southwest of China. In our study we suggested that sperm quality could decrease even before antineoplastic treatment and sperm banking prior to treatment should be strongly recommended for male cancer patients. 

scholarly journals The Impact of Radiofrequency Waves on Male Infertility: A Systematic Review

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Leili Darvish ◽  
Azadeh Amraee ◽  
Marjan Akhavan Amjadi ◽  
Zahra Atarodi Kashani ◽  
Masoumeh Ghazanfarpour ◽  
...  
Keyword(s):  
Systematic Review ◽  
Male Infertility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Semen Parameters ◽  
Cochrane Library ◽  
Negative Effects ◽  
The Impact

Context: As the use of cellphones and other electronic devices increases, concerns about the possible effect of radiofrequency waves on health are growing. Long-term use of the cellphone may have negative effects on sperm quality. Objectives: The purpose of this research was to examine men's infertility due to the effect of radiofrequency waves. Methods: In this systematic review, language restrictions were not considered in searching the databases. Cochrane Library, Google Scholar, PubMed, EMBASE, ProQuest, CINAHL, Science Direct, MEDLINE, and Scopus were used to obtain the data from them. All data were scanned from the year 2000 until 2019. Papers selected for retrieval were evaluated by the Newcastle-Ottawa and CONSORT scales. Results: A total of 14 articles that met the inclusion criteria were ultimately assessed. Motile sperm, sperm vitality and membrane integrity, morphology, volume, total sperm count, sperm concentration, and sperm fertility were found to be influenced by radiofrequency waves. Conclusions: The results showed that RF has detrimental effects on semen parameters and due to an increase in RF wave use currently and its role in male infertility, giving information to men about adverse complications of RF is necessary. Further studies are needed to design the less harmful devices.

scholarly journals Evaluation of the Efficiency of Two Different Freezing Media and Two Different Protocols to Preserve Human Spermatozoa from Cryoinjury

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Gemma Fabozzi ◽  
Maria Flavia Starita ◽  
Emilia Rega ◽  
Alessandra Alteri ◽  
Antonio Colicchia ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Human Sperm ◽  
Human Spermatozoa ◽  
Sperm Cryopreservation ◽  
Progressive Motility ◽  
Direct Addition ◽  
Sperm Sample ◽  
Sperm Survival ◽  
Better Than

It is universally recognized that cryopreservation impairs sperm quality. In order to improve postthawing sperm survival and motility, media of different composition and different protocols have been proposed. However, no clear evidence is available to understand which are the most efficient protocol and medium for sperm cryopreservation. The present study evaluates the efficiency of two different cryopreservation protocols and two common freezing media (FM) containing different cryoprotectants (CPs), TEST Yolk Buffer (TYB) and Sperm Freeze (SF), to preserve human sperm quality. Our data suggest that TYB is better than SF both in terms of postthaw viability and in terms of progressive motility, while the direct addition of FM to the sperm sample resulted in the most efficient protocol in terms of postthaw viability but not in terms of progressive motility.

scholarly journals Male Infertility and Sperm DNA Fragmentation

2018 ◽  
Vol 6 (8) ◽  
pp. 1342-1345 ◽  
Author(s):  
Afrim Zeqiraj ◽  
Sheqibe Beadini ◽  
Nexhbedin Beadini ◽  
Hesat Aliu ◽  
Zafer Gashi ◽  
...  
Keyword(s):  
Male Infertility ◽  
Dna Fragmentation ◽  
Reproductive Age ◽  
Sperm Parameters ◽  
Semen Parameters ◽  
Sperm Chromatin ◽  
Control Group ◽  
P Value ◽  
Sperm Dna Fragmentation ◽  
Factors Affecting

BACKGROUND: One of the main factors affecting male infertility is DNA fragmentation in sperm. Male infertility is a heterogeneous group of disorders, known causes account for only 30-50%, and unknown cause (idiopathic) constitute the rest. Infertility involves nearly 15% of couples in the reproductive age, and only the male problem involves about 40% of the problems.AIM: We have studied our DNA damage to sperm cells of a group of infertile males (113 patients) with abnormal sperm parameters (oligoasthenospermia and oligospermia) and a group of male patients (80 patients) with normal semen parameters (normospermia) to document whether the Sperm Chromatin Dispersion (SCD) analysis could increase the information obtained from the sperm routine analysis to explain the causes of infertility.MATERIALS: A group of 193 patients were analysed, 113 patients in the working group and 80 patients in the control group were screened. The ejaculate samples were taken by the patient to whom the reason for the analysis was explained. All patients were from the Republic of Kosovo. Samples are collected from 2014/2018. Sperm Chromatin Dispersion (SCD) analyses in the ejaculate were analysed by the Biolab Zafi laboratory in Peja.RESULTS: Clinical data were compared between the two groups by one-way ANOVA, mean ± SD, student's t-test. A p-value of less than P < 0.05% was considered statistically significant. Outcomes: In our study, we have gained significant (P < 0.05) results in the workgroup and the control group across all hormonal parameters, sperm parameters, and fragmented DNA in the sperm.CONCLUSION: Based on our obtained results we can conclude that DNA fragmentation in spermatozoa is useful in the selection of unsuitable DNA sperm for use in ART methods. We conclude that our DNA fragmentation analysis results are encouraging and can be used for diagnostic purposes in determining male infertility.

The influence of cigarette smoking on human sperm quality and DNA fragmentation

Toxicology ◽  
2006 ◽  
Vol 223 (1-2) ◽  
pp. 54-60 ◽  
Author(s):  
Sandrine Sepaniak ◽  
Thierry Forges ◽  
Hubert Gerard ◽  
Bernard Foliguet ◽  
Marie-Christine Bene ◽  
...  
Keyword(s):  
Cigarette Smoking ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Human Sperm

scholarly journals Nuclear staining identifies two populations of human sperm with different DNA fragmentation extent and relationship with semen parameters

2008 ◽  
Vol 23 (5) ◽  
pp. 1035-1043 ◽  
Author(s):  
M. Muratori ◽  
S. Marchiani ◽  
L. Tamburrino ◽  
V. Tocci ◽  
P. Failli ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Human Sperm ◽  
Semen Parameters ◽  
Nuclear Staining ◽  
Two Populations

scholarly journals Protective effect of vitamin E on sperm parameters, chromatin quality, and DNA fragmentation in mice treated with different doses of ethanol: An experimental study

2021 ◽  
Author(s):  
Mohamad Reza Doostabadi ◽  
Mohammadmehdi Hassanzadeh-taheri ◽  
Mahmoud Asgharzadeh ◽  
Masoomeh Mohammadzadeh
Keyword(s):  
Vitamin E ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Dna Structure ◽  
Basal Diet ◽  
Sperm Parameters ◽  
Sperm Chromatin ◽  
Control Group ◽  
Protective Effects ◽  
Normal Morphology

Background: Excessive consumption of alcohol induces an increase in oxidative stress production and can lead to detrimental effects on the male reproductive system. Objective: To evaluate the possible protective effects of coadministration of vitamin (vit) E on the detrimental changes in the sperm quality of mice administered ethanol. Materials and Methods: Fifty-four BALB/c mice were categorized into nine groups (n = 6/each). The control group received a basal diet while the eight experimental groups received ethanol 10%; ethanol 20%; vit. E 100 mg; vit. E 200 mg; ethanol 10% + vit. E 100 mg; ethanol 10% + vit. E 200 mg; ethanol 20% + vit. E 100 mg; ethanol 20% + vit. E 200 mg. After 35 days, the sperm parameters and sperm chromatin were assessed. Results: The results demonstrated a significant reduction in the motility rate, normal morphology rate, viability rate, increase in abnormal DNA structure and packaging (TB staining), and DNA damage (TUNEL) in ethanol consumer groups. In addition, the findings showed a significant increase in the aforementioned parameters in ethanoland vit. E-consumer groups compared to the ethanol-only consumer groups. The ethanol group received 20% of the most damage among the groups. The group receiving vit. E 100 mg and those receiving ethanol 10% + vit. E 200 mg gained the highest benefit among the groups. Conclusion: Sperm forward progressive motility, normal morphology rate, and viability decreased in the ethanol groups. Also, the rates of spermatozoa with abnormal DNA structure and DNA fragmentation increased in the ethanol groups. Our findings revealed that the coadministration of vit. E and ethanol can protect destructive changes in DNA structure and damage. Key words: Ethanol, Sperm parameters, Vitamin E.

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