Effects of subchronic benzo(a)pyrene exposure on neurotransmitter receptor gene expression in the rats hippocampus related with spatial learning and memory change

Toxicology ◽  
2011 ◽  
Vol 289 (2-3) ◽  
pp. 83-90 ◽  
Author(s):  
Chongying Qiu ◽  
Shuqun Cheng ◽  
Yinyin Xia ◽  
Bin Peng ◽  
Qian Tang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Learning And Memory ◽  
Spatial Learning ◽  
Receptor Gene ◽  
Spatial Learning And Memory ◽  
Neurotransmitter Receptor ◽  
Memory Change ◽  
Receptor Gene Expression

scholarly journals Extended access oxycodone self-administration and neurotransmitter receptor gene expression in the dorsal striatum of adult C57BL/6 J mice

2013 ◽  
Vol 231 (7) ◽  
pp. 1277-1287 ◽  
Author(s):  
Yong Zhang ◽  
Brandan Mayer-Blackwell ◽  
Stefan D. Schlussman ◽  
Matthew Randesi ◽  
Eduardo R. Butelman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Receptor Gene ◽  
Dorsal Striatum ◽  
Self Administration ◽  
Neurotransmitter Receptor ◽  
Extended Access ◽  
Receptor Gene Expression

scholarly journals Milk Exosomes Cross the Blood-Brain Barrier in Murine Cerebral Cortex Endothelial Cells and Promote Dendritic Complexity in the Hippocampus and Brain Function in C57BL/6J Mice

2021 ◽  
Author(s):  
Fang Zhou ◽  
Pearl Ebea ◽  
Ezra Mutai ◽  
Sonal Sukreet ◽  
Shya Navazesh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Brain Development ◽  
Learning And Memory ◽  
Kainic Acid ◽  
Spatial Learning ◽  
Granule Cells ◽  
Spatial Learning And Memory ◽  
Adult Mice ◽  
Dendritic Complexity

Background: Human milk contains large amounts of exosomes (MEs) and their regulatory microRNA cargos, whereas infant formulas contain only trace amounts of MEs and microRNAs. Breastfeeding has been implicated in optimal brain development but experimental evidence linking ME intake with brain development is limited. Objectives: We assessed the transport of MEs across the blood-brain barrier (BBB) and ME accumulation in distinct regions of the brain in brain endothelial cells and suckling mice. We further assessed BME-dependent gene expression profiles and effects on the dendritic complexity of hippocampal granule cells and phenotypes of BME depletion in neonate, juvenile and adult mice. Methods: The transfer of MEs across the BBB was assessed by using bovine MEs labeled with FM4-64 or loaded with IRDye-labeled miR-34a in murine brain endothelial bEnd.3 cell monolayers and dual chamber systems, and in wild-type newborn pups fostered to exosome and cargo tracking (ECT) dams that express MEs endogenously labeled with a CD63-eGFP fusion protein for subsequent analysis by serial two-photon tomography and staining with anti-eGFP antibodies. Effects of MEs on gene expression and dendritic architecture of granule cells was analyzed in hippocampi from juvenile mice fed exosome and RNA-depleted (ERD) and exosome and RNA-sufficient (ERS) diets by using RNA-sequencing analysis and Golgi-Cox staining followed by integrated neuronal tracing and morphological analysis of neuronal dendrites, respectively. Spatial learning and severity of kainic acid-induced seizures were assessed in mice fed ERD and ERS diets. Results: bEnd.3 cells internalized MEs by using a saturable transport mechanism and secreted miR-34a across the basal membrane. MEs penetrated the entire brain in fostering experiments; major regions of accumulation included the hippocampus, cortex and cerebellum. Two hundred ninety-five genes were differentially expressed in hippocampi from male mice fed ERD and ERS diets; high-confidence gene networks included pathways implicated in axon guidance and calcium signaling. Only one gene was differentially expressed in females fed the experimental diets. Juvenile pups fed the ERD diet had reduced dendritic complexity of dentate granule cells in the hippocampus, scored nine-fold lower in the Barnes maze test of spatial learning and memory (P < 0.01), and the severity of seizures was 5-fold higher following kainic acid administration in adult mice fed the ERD diet compared to mice fed the ERS diet (P < 0.01). Conclusions: MEs cross the BBB and contribute toward optimal neuronal development, spatial learning and memory, and resistance to kainic acid-induced seizures in mice.

Spatial learning and memory function-related gene expression in the hippocampus of mouse exposed to nanoparticle-rich diesel exhaust

2008 ◽  
Vol 29 (6) ◽  
pp. 940-947 ◽  
Author(s):  
Tin-Tin Win-Shwe ◽  
Shoji Yamamoto ◽  
Yuji Fujitani ◽  
Seishiro Hirano ◽  
Hidekazu Fujimaki
Keyword(s):  
Gene Expression ◽  
Learning And Memory ◽  
Spatial Learning ◽  
Diesel Exhaust ◽  
Memory Function ◽  
Spatial Learning And Memory ◽  
Related Gene

Maternal and postweaning high-fat diets disturb hippocampal gene expression, learning, and memory function

2014 ◽  
Vol 306 (8) ◽  
pp. R527-R537 ◽  
Author(s):  
Kathleen C. Page ◽  
Elizabeth K. Jones ◽  
Endla K. Anday
Keyword(s):  
Gene Expression ◽  
Learning And Memory ◽  
Spatial Learning ◽  
Memory Function ◽  
Saturated Fat ◽  
Spatial Learning And Memory ◽  
Adult Males ◽  
High Fat ◽  
High Fat Diets ◽  
Fat Diets

We tested the hypothesis that excess saturated fat consumption during pregnancy, lactation, and/or postweaning alters the expression of genes mediating hippocampal synaptic efficacy and impairs spatial learning and memory in adulthood. Dams were fed control chow or a diet high in saturated fat before mating, during pregnancy, and into lactation. Offspring were weaned to either standard chow or a diet high in saturated fat. The Morris Water Maze was used to evaluate spatial learning and memory. Open field testing was used to evaluate motor activity. Hippocampal gene expression in adult males was measured using RT-PCR and ELISA. Offspring from high fat-fed dams took longer, swam farther, and faster to try and find the hidden platform during the 5-day learning period. Control offspring consuming standard chow spent the most time in memory quadrant during the probe test. Offspring from high fat-fed dams consuming excess saturated fat spent the least. The levels of mRNA and protein for brain-derived neurotrophic factor and activity-regulated cytoskeletal-associated protein were significantly decreased by maternal diet effects. Nerve growth factor mRNA and protein levels were significantly reduced in response to both maternal and postweaning high-fat diets. Expression levels for the N-methyl-d-aspartate receptor (NMDA) receptor subunit NR2B as well as synaptophysin were significantly decreased in response to both maternal and postweaning diets. Synaptotagmin was significantly increased in offspring from high fat-fed dams. These data support the hypothesis that exposure to excess saturated fat during hippocampal development is associated with complex patterns of gene expression and deficits in learning and memory.

scholarly journals Self administration of oxycodone by adolescent and adult mice affects striatal neurotransmitter receptor gene expression

Neuroscience ◽  
2014 ◽  
Vol 258 ◽  
pp. 280-291 ◽  
Author(s):  
B. Mayer-Blackwell ◽  
S.D. Schlussman ◽  
E.R. Butelman ◽  
A. Ho ◽  
J. Ott ◽  
...  
Keyword(s):  
Gene Expression ◽  
Receptor Gene ◽  
Self Administration ◽  
Neurotransmitter Receptor ◽  
Adult Mice ◽  
Receptor Gene Expression

scholarly journals Effects of Tarragon Hydroalcoholic Extract and Coumarin On Memory, Tissue Index and GABAA Receptor Gene Expression in The Hippocampus of Male Rats

2021 ◽  
Author(s):  
Nasrin Heidarieh ◽  
Maryam Najafifard ◽  
Ali Haeri Rohani ◽  
Akram Eidi
Keyword(s):  
Gene Expression ◽  
Learning And Memory ◽  
Gabaa Receptor ◽  
Retention Test ◽  
Receptor Gene ◽  
Male Rats ◽  
Memory Retention ◽  
Hydroalcoholic Extract ◽  
Genes Expression ◽  
Receptor Gene Expression

Abstract Background and objective: Learning and memory are necessary for survival. The hippocampus plays a significant role in learning process. GABAA receptors in the hippocampus are effective in learning and memory mechanism. The present study effect of tarragon hydroalcoholic extract and coumarin on memory, tissue index and GABAA receptor gene expression in the hippocampus of male rats. Methodology: 56 Wistar rats were used and randomized in 7 groups (N = 8). These groups included the intact, receiving DMSO, receiving tarragon hydroalcoholic extract doses of 25, 50 and 100 mg/kg and receiving coumarin dose of 3, 5 mg/kg. They have undergone treatment intraperitoneally once a day for two weeks. The shuttle box was used for the memory retention test. The rats were killed according to the research ethical codes after the tests were done. When the brains of rats were removed, 4 brains in each group were chosen for the histological test using Nissl staining. In the other four brains, the hippocampus was removed immediately. The hippocampus was located in a microtube and was frozen by liquid nitrogen. Finally, a gene expression test was performed using real-time PCR. Results the findings of the present study reveal that there was no significant difference between of solvent recipients and the intact group in the memory retention test, the number of healthy hippocampal pyramidal neurons, and the expression of the GABAA gene. The treated groups with various doses of hydroalcoholic extract of tarragon and coumarin showed decreased in the memory retention test and the number of healthy pyramidals neurons as well as a significant increase in GABAA- α5 and GABAA- α2 genes expression compared to the group receiving solvent. Conclusion Tarragon hydroalcoholic extract and coumarin affects memory impairment through increasing the GABAA-α5 and GABAA-α2 genes expression and decreasing the number of healthy hippocampal neurons.

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