Detection of Purkinje cell loss following drug exposures to developing rat pups using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for calbindin-D28k mRNA expression

2004 ◽  
Vol 150 (3) ◽  
pp. 325-334 ◽  
Author(s):  
Yun Ge ◽  
Scott M. Belcher ◽  
Dwight R. Pierce ◽  
Kim E. Light
Keyword(s):  
Polymerase Chain Reaction ◽  
Cell Loss ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Rat Pups ◽  
Purkinje Cell Loss ◽  
Calbindin D28k ◽  
Polymerase Chain ◽  
Developing Rat

Prospective Multi-Institutional Study of Reverse Transcriptase Polymerase Chain Reaction for Molecular Staging of Melanoma

2006 ◽  
Vol 24 (18) ◽  
pp. 2849-2857 ◽  
Author(s):  
Charles R. Scoggins ◽  
Merrick I. Ross ◽  
Douglas S. Reintgen ◽  
R. Dirk Noyes ◽  
James S. Goydos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Peripheral Blood ◽  
Prognostic Significance ◽  
Disease Free Survival ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Molecular Staging ◽  
Polymerase Chain

Purpose To evaluate the prognostic significance of molecular staging using reverse transcriptase polymerase chain reaction (RT-PCR) in detecting occult melanoma cells in sentinel lymph nodes (SLNs) and circulating bloodstream. Patients and Methods In this multicenter study, eligibility criteria included patient age 18 to 71 years, invasive melanoma ≥ 1.0 mm Breslow thickness, and no clinical evidence of metastasis. SLN biopsy and wide excision of the primary tumor were performed. SLNs were examined by serial-section histopathology and S-100 immunohistochemistry. A portion of each SLN was frozen for RT-PCR. In addition, RT-PCR was performed on peripheral-blood mononuclear cells (PBMCs). RT-PCR analysis was performed using four markers: tyrosinase, MART1, MAGE3, and GP-100. Disease-free survival (DFS), distant–DFS (DDFS), and overall survival (OS) were analyzed. Results A total of 1,446 patients with histologically negative SLNs underwent RT-PCR analysis. At a median follow-up of 30 months, there was no difference in DFS, DDFS, or OS between the RT-PCR–positive (n = 620) and RT-PCR–negative (n = 826) patients. Analysis of PBMC from 820 patients revealed significant differences in DFS and DDFS, but not OS, for patients with detection of more than one RT-PCR marker in peripheral blood. Conclusion In this large, prospective, multi-institutional study, RT-PCR analysis on SLNs and PBMCs provides no additional prognostic information beyond standard histopathologic analysis of SLNs. Detection of more than one marker in PBMC is associated with a worse prognosis. RT-PCR remains investigational and should not be used to direct adjuvant therapy at this time.

scholarly journals REAL TIME POLYMERASE CHAIN REACTION (RT-PCR) FOR DNA PIG CONTAMINATION IDENTIFICATION SAUSAGE IN SIX DISTRICT PANDEGLANG BANTEN

2021 ◽  
Vol 1 (1) ◽  
pp. 81-88
Author(s):  
Hadi Susilo
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Meat Product ◽  
Meat Products ◽  
Pcr Analysis ◽  
Processed Meat ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Dna Purity ◽  
Polymerase Chain

Sausage is a meat product processed that is popular food especially in Pandeglang, Banten Province. The importance of halal certificates or the existence of the MUI (Indonesian Ulama Council) halal logo for processed meat products makes Muslim people confident to consume them. The aim this research was to identify pig DNA contamination in sausage products in six  districts in Pandeglang without the MUI halal labels using RT-PCR (Real Time-Polymerase Chain Reaction). RT PCR that can calculate to pig to fill these sample free from pig contamination. This research was divided into two stage, the first stage is extracted or carried out DNA and the second stage is RT PCR analysis. The results of the DNA purity test on sausage samples had DNA purity values ​​of 1.84-1.9 (A260 / A280) and resulted in sample concentrations ranging from 37.8 to 102.5 ng / µl.  The only amplification on the FAM curve was in the positive control pig.  the Cq value ranges from 30 - 31.29. The results of RT PCR on sausage samples in the district area in Pandeglang Banten did not detect the presence of pig DNA.

scholarly journals STUDI KASUS INFEKSI TILAPIA LAKE VIRUS (TiLV) PADA IKAN NILA (Oreochromis niloticus)

2018 ◽  
Vol 13 (1) ◽  
pp. 85 ◽  
Author(s):  
Isti Koesharyani ◽  
Lila Gardenia ◽  
Zakiyah Widowati ◽  
Khumaira Khumaira ◽  
Dita Rustianti
Keyword(s):  
Polymerase Chain Reaction ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Rna Virus ◽  
Pcr Analysis ◽  
Sequencing Analysis ◽  
Accession Number ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Ikan nila atau Oreochromis niloticus merupakan ikan konsumsi masyarakat di Indonesia. Kasus kematian massal ikan nila terjadi di beberapa lokasi budaya di Jawa, Lombok, dan Sumatera yang disebabkan oleh infeksi Orthomyxovirus, dan disebut sebagai Tilapia Lake Virus (TiLV). Tujuan penelitian ini adalah untuk mendeteksi adanya infeksi TiLV dengan metode semi-nested Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) pada kasus kematian massal ikan nila. Lokasi pengambilan sampel di Desa Sigerongan Kecamatan Lingsar, Lombok, Nusa Tenggara Barat. Analisis deteksi RT-PCR menggunakan sampel organ otak, ginjal, limpa, dan hati, selanjutnya dilakukan sekuensing. Hasil pengamatan terhadap gejala klinis terhadap ikan nila moribund terlihat kondisi mata yang buram/katarak, serta cekung, abrasi kulit, serta perubahan warna tubuh menjadi lebih gelap. Hasil analisis RT-PCR menunjukkan bahwa kejadian kematian massal pada ikan nila suspektif diakibatkan oleh infeksi RNA virus TiLV. Analisis sekuensing menunjukkan bahwa TiLV dari sampel ikan nila di Lombok mempunyai kesamaan identitas genetik 97% dengan TiLV asal Israel (Genebank Accession Number KU 751816.1).Oreochromis niloticus is the main consumption fish commodity in Indonesia. The mortality cases of Nile tilapia have occurred in several culture sites in Java, Lombok, and Sumatra due to the infection of Orthomyxovirus, Tilapia Lake Virus (TiLV). The purpose of this study was to detect the presence of TiLV infection in mass mortality case of Nile tilapia culture using the semi-nested Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Fish samples were sourced from Segerongan Village Lingsar District, Lombok, West Nusa Tenggara. For RT-PCR analysis, samples from fish brain, kidney, spleen, and liver were collected and treated for sequencing analysis. The visual observation on the moribund tilapia had found several specific clinical symptoms such as eye cataract with sunken eyes, skin abrasion, and darkened body coloration. The result of RT-PCR analysis showed that mass mortalities of tilapia fish had been suspective caused by the infection TiLV RNA virus. The sequencing analysis showed that TiLV samples from Lombok have a genetic similarity of 97% with TiLV from Israeli (Genebank Accession Number KU 751816.1).

Use of the polymerase chain reaction for the detection of enteroviruses in river and marine recreational waters

1995 ◽  
Vol 31 (5-6) ◽  
pp. 337-344 ◽  
Author(s):  
A. P. Wyn-Jones ◽  
R. Pallin ◽  
J. Sellwood ◽  
D. Tougianidou
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Recreational Waters ◽  
Guanidinium Thiocyanate ◽  
Polymerase Chain ◽  
Seawater Samples ◽  
Cell Culture Passage

The polymerase chain reaction (PCR) was used to detect human enteroviruses in river and seawater samples in comparison with cell culture analysis. Extraction of the RNA from the sample was achieved by adsorption to size-fractionated silica in the presence of guanidinium thiocyanate. Good correlation was obtained between the two methods. For most samples it was not found necessary to carry out an initial cell culture passage prior to RT-PCR analysis, and RT-PCR was found to be as sensitive and more rapid than cell culture.

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