Cutaneous wound healing in the EpiDerm-FT full thickness in vitro human skin model

Cutaneous wound healing is a complex multi-stage process involving direct and indirect cell communication events with the aim of efficiently restoring the barrier function of the skin. One key aspect in cutaneous wound healing is associated with cell movement and migration into the physically, chemically and biologically injured area resulting in wound closure. Understanding the conditions under which cell migration is impaired and elucidating the cellular and molecular mechanisms that improve healing dynamics is therefore crucial in devising novel therapeutic strategies to elevate patient suffering, reduce scaring and eliminate chronic wounds. Following the global trend towards automation, miniaturization and integration of cell-based assays into microphysiological systems, conventional wound healing assays such as the scratch assay or cell exclusion assay have recently been translated and improved using microfluidics and lab-on-a-chip technologies. These miniaturized cell analysis systems allow precise spatial and temporal control over a range of dynamic microenvironmental factors including shear stress, biochemical and oxygen gradients to create more reliable in vitro models that resemble the in vivo microenvironment of a wound more closely on a molecular, cellular, and tissue level. The current review provides (a) an overview on the main molecular and cellular processes that take place during wound healing, (b) a brief introduction into conventional in vitro wound healing assays, and (c) a perspective on future cutaneous and vascular wound healing research using microfluidic technology.

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The Effect of 17β-Estradiol Administration on Cutaneous Wound Healing in 24-Week Ovariectomized Female Mice

Journal of Hormones ◽

10.1155/2014/234632 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Kanae Mukai ◽

Yukari Nakajima ◽

Tamae Urai ◽

Emi Komatsu ◽

Kana Takata ◽

...

Keyword(s):

Wound Healing ◽

Inflammatory Response ◽

Cutaneous Wound Healing ◽

Full Thickness ◽

Estrogen Replacement ◽

Wound Area ◽

Cutaneous Wound ◽

Female Mice ◽

Exogenous Estrogen ◽

17Β Estradiol

Estrogen replacement promotes cutaneous wound healing in 8–10-week young ovariectomized female mice. However, research using aged ovariectomized female mice has not been reported, to the best of our knowledge. Therefore, we investigated the effect of 17β-estradiol on cutaneous wound healing using 24-week middle-aged ovariectomized female mice. Twenty-week-old female mice were divided into three groups: medication with 17β-estradiol after ovariectomy (OVX + 17β-estradiol), ovariectomy (OVX), and sham (SHAM). After 4 weeks, the mice received two full-thickness wounds. Then, the OVX + 17β-estradiol group was administered 17β-estradiol at 0.01 g/day until healing. The ratio of wound area in the OVX + 17β-estradiol group was significantly decreased compared with that in the OVX group. The numbers of neutrophils and macrophages in the OVX + 17β-estradiol group were significantly smaller than those in the OVX group. In addition, the ratio of myofibroblasts in the OVX + 17β-estradiol group was significantly higher than that in the OVX group. These data suggested that exogenous continuous 17β-estradiol administration promotes cutaneous wound healing in 24-week OVX female mice by reducing wound area, shortening inflammatory response, and promoting wound contraction. However, it is unclear whether the effect of exogenous estrogen on wound healing outweighs the delay of wound healing due to advanced age.

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ADM Scaffolds Generate a Pro-regenerative Microenvironment During Full-Thickness Cutaneous Wound Healing Through M2 Macrophage Polarization via Lamtor1

Frontiers in Physiology ◽

10.3389/fphys.2018.00657 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 6

Author(s):

Chengmin He ◽

Zhi Yang ◽

Ying Jin ◽

Xiaoyang Qi ◽

Jin Chu ◽

...

Keyword(s):

Wound Healing ◽

Macrophage Polarization ◽

M2 Macrophage ◽

Cutaneous Wound Healing ◽

Full Thickness ◽

Cutaneous Wound ◽

M2 Macrophage Polarization

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Novel synthesis of Falcaria vulgaris leaf extract conjugated copper nanoparticles with potent cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing activities under in vitro and in vivo condition

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2019.111556 ◽

2019 ◽

Vol 197 ◽

pp. 111556 ◽

Cited By ~ 27

Author(s):

Mohammad Mahdi Zangeneh ◽

Hori Ghaneialvar ◽

Mohsen Akbaribazm ◽

Mohamad Ghanimatdan ◽

Naser Abbasi ◽

...

Keyword(s):

Wound Healing ◽

Copper Nanoparticles ◽

Leaf Extract ◽

Cutaneous Wound Healing ◽

Cutaneous Wound ◽

Novel Synthesis ◽

Falcaria Vulgaris

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Effect of Skin Model on In Vitro Performance of an Adhesive Dermally Applied Microarray Coated with Zolmitriptan

Journal of Pharmaceutics ◽

10.1155/2018/7459124 ◽

2018 ◽

Vol 2018 ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

Mahmoud Ameri ◽

Hayley Lewis ◽

Paul Lehman

Keyword(s):

Human Skin ◽

Ex Vivo ◽

Full Thickness ◽

Artificial Membrane ◽

Skin Model ◽

Hydrophobic Membrane ◽

Thickness Skin ◽

In Vitro Performance

Franz cell studies, utilizing different human skin and an artificial membrane, evaluating the influence of skin model on permeation of zolmitriptan coated on an array of titanium microprojections, were evaluated. Full thickness and dermatomed ex vivo human skin, as well as a synthetic hydrophobic membrane (Strat-M®), were assessed. It was found that the choice of model demonstrated different absorption kinetics for the permeation of zolmitriptan. For the synthetic membrane only 11% of the zolmitriptan coated dose permeated into the receptor media, whilst for the dermatomed skin 85% permeated into the receptor. The permeation of zolmitriptan through full thickness skin had a significantly different absorption profile and time to maximum flux in comparison to the dermatomed skin and synthetic model. On the basis of these results dermatomed skin may be a better estimate of in vivo performance of drug-coated metallic microprojections.

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