Production and issuing of pooled granulocytes concentrates from whole blood donations in France

Background: Hemovigilance like quality systems and audits have become an integral part of Blood Transfusion Services in the developed countries and has contributed greatly to its development. Hemovigilance begins with donors and must enable the collection of information on reactions occurring during the donation of blood, selections of donors and to prevent such incidents. The aim of study was to help identify the trends of adverse events , occurring in blood donors at a tertiary-care hospital, to recommend best practices to improve donor care and safety Materials and Methods: This record-based study was conducted on all adverse events related to allogenic whole blood donations performed over 24 months. All whole blood donations were analyzed. All adverse events occurring during or at the end of the donation were noted using a standardized format and analyzed determining significance at p<0.05. Results: Overall rate was 0.3% with vasovagal reactions constituting 82%, and 18% mild syncopal reactions (p<0.001). Immediate vasovagal reaction with injury was very rare (0.007%). Vasovagal reactions showed a significant association with young age, female gender, first time donation status. Mean age of persons recording adverse effects was 30.23 ± 7.49 years as compared to those without adverse effects, 31.14 ± 8.56 years. Conclusion: Donor safety is an essential perquisite to increase voluntary blood donation. AE analysis helps in identifying the blood donors at risk of AE, applying appropriate motivational strategies, predonation counseling, care during and after donation, developing guidelines and hemovigilance programme in countries with limited resources. DOI: http://dx.doi.org/10.3126/jpn.v3i6.8993 Journal of Pathology of Nepal (2013) Vol. 3, 459-463

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The results of nucleic acid testing for viruses in individual donor test and its importance for the safety of blood

Medicina ◽

10.3390/medicina44100099 ◽

2008 ◽

Vol 44 (10) ◽

pp. 791 ◽

Cited By ~ 1

Author(s):

Vytenis Kalibatas

Keyword(s):

Infectious Disease ◽

Hepatitis C ◽

Nucleic Acid ◽

Hepatitis B ◽

Whole Blood ◽

Blood Donations ◽

Disease Marker ◽

Disease Markers ◽

First Time ◽

Individual Donor

The aim of the study was to evaluate the results of nucleic acid testing for viruses in an individual donor test in National Blood Center; the objectives – to analyze the prevalence of infectious disease markers per 100 seronegative remunerated and non-remunerated, first-time and regular whole-blood donations and to assess the odds ratio in detecting the infectious disease markers among remunerated and non-remunerated donations. Materials and methods. All seronegative (for compulsory hepatitis B surface antigen, antibodies against hepatitis C, and antibodies against HIV-1/2 tests) whole-blood donations were tested by Procleix Ultrio (Tigris, Chiron) system at the National Blood Center in 2005–2007 in order to identify HIV-1, hepatitis C, and hepatitis B viruses. Results. There were 152229 seronegative whole-blood donations tested by nucleic acid test of viruses in individual donor tests (ID-NAT). In 152146 cases, no infectious disease marker was found, and in 83 cases (or 0.05% of all seronegative whole blood donations), infectious disease markers were determined and confirmed. The prevalences of hepatitis C virus (determined by HCV-NAT method) per 100 seronegative blood donations were as follows: 0.061 among first-time remunerated donations and 0.042 among regular remunerated donations. The prevalences of hepatitis B virus (determined by HBV-NAT method) per 100 seronegative blood donations were as follows: 0.111 among first-time remunerated donations, 0.062 among regular remunerated donations, 0.014 among first-time non-remunerated donations, and 0.005 among regular non-remunerated donations. The remunerated donations showed the higher odds ratios in determining the infectious disease marker by ID-NAT test, comparing with non-remunerated ones. Conclusions. 1. The prevalence of hepatitis B and hepatitis C viruses, determined by ID-NAT test, per 100 seronegative whole-blood donations is statistically significantly higher in remunerated donations. 2. The remunerated donations had the higher odds ratios in determining the infectious disease marker by ID-NAT test, comparing with non-remunerated ones. 3. In order to maximize the safety of blood and blood products, the continuity of promotion of non-remunerated whole-blood donations program should be ensured, and a compulsory blood donor testing for nucleic acids of viruses in an individual donor test should be introduced.

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Quality of frozen transfusable plasma prepared from whole blood donations in Canada: An update

Transfusion and Apheresis Science ◽

10.1016/j.transci.2013.06.012 ◽

2013 ◽

Vol 49 (3) ◽

pp. 440-446 ◽

Cited By ~ 7

Author(s):

William P. Sheffield ◽

Varsha Bhakta ◽

Kimberley Talbot ◽

Edward L.G. Pryzdial ◽

Craig Jenkins

Keyword(s):

Whole Blood ◽

Blood Donations

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Preventing Platelet-Derived Microparticle Formation—and Possible Side Effects—With Prestorage Leukofiltration of Whole Blood

Archives of Pathology & Laboratory Medicine ◽

10.5858/134.5.771 ◽

2010 ◽

Vol 134 (5) ◽

pp. 771-775 ◽

Cited By ~ 5

Author(s):

Akiko Sugawara ◽

Kenneth E. Nollet ◽

Kentaro Yajima ◽

Shunnichi Saito ◽

Hitoshi Ohto

Keyword(s):

Flow Cytometry ◽

Side Effects ◽

Whole Blood ◽

Red Cell ◽

Blood Donations ◽

Peak Number ◽

To Come ◽

Microparticle Formation ◽

Stored Blood ◽

Leukocyte Filtration

Abstract Context.—Platelet-derived microparticles (PDMPs) probably function in hemostasis, thrombosis, inflammation, and transfusion-related immunomodulation. Objective.—To compare PDMP levels of leukocyte-filtered and unfiltered whole blood during storage. Design.—Ten whole blood donations were collected and processed. Half of each collection was filtered, half remained unfiltered, and both halves were measured for red cell, white cell, and platelet (PLT) content before storage. Samples were drawn on days 0, 1, 2, 3, 5, 7, 14, 21, 28, and 35 and analyzed by flow cytometry. Results.—Leukocyte filtration lowered prestorage PDMP and PLT counts by an average of 72% and 99%, respectively. Prestorage PDMP counts were 123 ± 51/µL in unfiltered whole blood supernatant versus 34 ± 18/µL after filtration. Prestorage PLT counts were 190 ± 49/µL in unfiltered whole blood supernatant versus 2 ± 4/µL after filtration. Moreover, PDMP and PLT counts in filtered whole blood remained low throughout storage, typically below 100/µL. In contrast, unfiltered whole blood PDMP- and PLT-gated events increased approximately 2 log during storage, with the peak number of PLT-gated events tending to coincide with the peak number of PDMP-gated events (4 donors) or to come after the peak number of PDMP-gated events (6 donors). Conclusions.—Leukocyte filtration of whole blood lowers prestorage PDMP and PLT counts. Platelet-derived microparticle and PLT counts remain low throughout 35 days of storage. In contrast, PDMP- and PLT-gated events increase significantly in unfiltered whole blood. The nature of PLT-gated events in stored blood warrants further investigation.

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