Pathogen inactivation of whole blood and red cell components: An overview of concept, design, developments, criteria of acceptability and storage lesion

2013 ◽  
Vol 49 (2) ◽  
pp. 357-363 ◽  
Author(s):  
Jerard Seghatchian ◽  
Jeffrey S. Putter
Keyword(s):  
Whole Blood ◽  
Red Cell ◽  
Concept Design ◽  
Pathogen Inactivation ◽  
Storage Lesion ◽  
Cell Components ◽  
And Storage

scholarly journals Quantification of Cell-Free DNA in Red Blood Cell Units in Different Whole Blood Processing Methods

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Andrew W. Shih ◽  
Vinai C. Bhagirath ◽  
Nancy M. Heddle ◽  
Jason P. Acker ◽  
Yang Liu ◽  
...  
Keyword(s):  
Patient Outcomes ◽  
Whole Blood ◽  
Regression Models ◽  
Processing Method ◽  
Red Cell ◽  
Storage Duration ◽  
Cell Free Dna ◽  
Free Dna ◽  
And Storage ◽  
Separate Regression

Background. Whole blood donations in Canada are processed by either the red cell filtration (RCF) or whole blood filtration (WBF) methods, where leukoreduction is potentially delayed in WBF. Fresh WBF red blood cells (RBCs) have been associated with increased in-hospital mortality after transfusion. Cell-free DNA (cfDNA) is released by neutrophils prior to leukoreduction, degraded during RBC storage, and is associated with adverse patient outcomes. We explored cfDNA levels in RBCs prepared by RCF and WBF and different storage durations. Methods. Equal numbers of fresh (stored ≤14 days) and older RBCs were sampled. cfDNA was quantified by spectrophotometry and PicoGreen. Separate regression models determined the association with processing method and storage duration and their interaction on cfDNA. Results. cfDNA in 120 RBC units (73 RCF, 47 WBF) were measured. Using PicoGreen, WBF units overall had higher cfDNA than RCF units (p=0.0010); fresh WBF units had higher cfDNA than fresh RCF units (p=0.0093). Using spectrophotometry, fresh RBC units overall had higher cfDNA than older units (p=0.0031); fresh WBF RBCs had higher cfDNA than older RCF RBCs (p=0.024). Conclusion. Higher cfDNA in fresh WBF was observed compared to older RCF blood. Further study is required for association with patient outcomes.

scholarly journals Effects of irradiation and leukoreduction on down-regulation of CXCL-8 and storage lesion in stored canine whole blood

2019 ◽  
Vol 20 (1) ◽  
pp. 72 ◽  
Author(s):  
Hayoung Yang ◽  
Woosun Kim ◽  
Junwoo Bae ◽  
Hyunwoo Kim ◽  
Sangki Kim ◽  
...  
Keyword(s):  
Whole Blood ◽  
Down Regulation ◽  
Storage Lesion ◽  
And Storage

scholarly journals Established and theoretical factors to consider in assessing the red cell storage lesion

Blood ◽  
2015 ◽  
Vol 125 (14) ◽  
pp. 2185-2190 ◽  
Author(s):  
James C. Zimring
Keyword(s):  
Blood Cells ◽  
Biological Effects ◽  
Storage Conditions ◽  
Red Cell ◽  
Blood Products ◽  
Storage Lesion ◽  
And Storage ◽  
Theoretical Considerations ◽  
Rbc Transfusion ◽  
Cell Storage

AbstractThe collection and storage of red blood cells (RBCs) is a logistical necessity to provide sufficient blood products. However, RBC storage is an unnatural state, resulting in complicated biological changes, referred to collectively as the “storage lesion.” Specifics of the storage lesion have been studied for decades, including alterations to cellular properties, morphology, molecular biology of carbohydrates, proteins and lipids, and basic metabolism. Recently, mass spectrometry–based “omics” technology has been applied to the RBC storage lesion, resulting in many new observations, the initial effects of which are more information than understanding. Meanwhile, clinical research on RBC transfusion is considering both the efficacy and also the potential untoward effects of transfusing stored RBCs of different ages and storage conditions. The myriad biological changes that have now been observed during the storage lesion have been extensively reviewed elsewhere. This article focuses rather on an analysis of our current understanding of the biological effects of different elements of the storage lesion, in the context of evolving new clinical understanding. A synopsis is presented of both established and theoretical considerations of the RBC storage lesion and ongoing efforts to create a safer and more efficacious product.

The clinical consequences of the red cell storage lesion

2001 ◽  
Vol 15 (2) ◽  
pp. 91-107 ◽  
Author(s):  
Alan Tinmouth ◽  
Ian Chin-Yee
Keyword(s):  
Red Cell ◽  
Storage Lesion ◽  
Clinical Consequences ◽  
Cell Storage

Viscosity and Red Cell Deformity in Arterial Disease

1979 ◽  
Author(s):  
G Cella ◽  
H de Haas ◽  
M Rampling ◽  
V Kakkar
Keyword(s):  
Vascular Disease ◽  
Blood Viscosity ◽  
Whole Blood ◽  
Arterial Disease ◽  
Plasma Viscosity ◽  
Control Group ◽  
Fibrinogen Concentration ◽  
Red Cell ◽  
Whole Blood Viscosity ◽  
Significant Difference

Haemorrheological factors have been shown to be affected in many kings of vascular disease. The present study was undertaken to correlate these factors in normal subjects and patients suffering from peripheral arterial disease. Twenty-two patients were investigated; they had moderate or severe intermittent claudication, extent of disease being confirmed by aorto-arteriography and ankle-systolic pressure studies. Twenty-five controls with no symptoms or signs of arterial disease were selected with comparable age and sex distribution. Whole blood viscosity was measured at shear rates of 230 secs-1 and 23 secs-lat 37°c using a Wells Brookfield cone plate microvisco meter. Plasma viscosity was also measured in an identical manner. Erythrocyte flexibility was measured by centrifuge technique and fibrinogen concentration as well as haematocrit by standard techniques. The fibrinogen concentration appeared to be the only significant parameter; the mean concentration in patients with peripheral vascular disease of 463 ± 73mg/l00ml in the control group ( < 0.05). Although whole blood viscosity was high in patients, when corrected to a common haematocrit, there was no significant difference between patients and controls. The same megative correlation was found for plasma viscosity. The red cell flexibility was found to be increased in patients as compared to the control group, but this effect appeared to be simply proportional to the fibrinogen concentration.

scholarly journals Effect of Leukoreduction on Hematobiochemical Parameters and Storage Hemolysis in Canine Whole Blood Units

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 925
Author(s):  
Maria Teresa Antognoni ◽  
Maria Luisa Marenzoni ◽  
Ambra Lisa Misia ◽  
Luca Avellini ◽  
Elisabetta Chiaradia ◽  
...  
Keyword(s):  
Whole Blood ◽  
Complete Blood Count ◽  
Membrane Resistance ◽  
Progressive Increase ◽  
Rbc Membrane ◽  
Morphological Index ◽  
The Difference ◽  
Hematobiochemical Parameters ◽  
And Storage ◽  
Individual Donor

Storage lesions (SLs) occur when the red blood cell quality is altered during the preservation of blood units. Pre-storage leukoreduction would limit the number of SLs. The aims of this study were to evaluate the effectiveness of a leukoreduction filter for human use and the effect of pre-storage leukoreduction on some ematobiochemical parameters in stored canine whole blood. Seven canine blood units were tested. Each one was divided into two units—one leukoreduced (LRWB) and one non-leukoreduced (nLRWB). On each unit, we determined the complete blood count (CBC), lactate-dehydrogenase (LDH), electrolytes (Na+, K+, Cl−), morphological index (MI) and hemolysis, on storage days 0, 7, 14, 21, 28, 35, and 42. Leukoreduction allowed a 98.30% recovery of the RBC count, retaining 99.69% and 94.91% of WBCs and PLTs, respectively. We detected a significant increase of LDH and MI with strongly higher values in nLRWB compared to LRWB. A progressive increase in electrolytes and LDH concentrations was observed as indices of stored hemolysis. LDH showed significantly lower values in LRWB units compared to nLRWB, suggesting its release from leukocytes. In the majority of units, hemolysis reached 1% on the 42nd day of storage. We assert the human leukoreduction filter effectiveness on canine whole blood, and we recommend using nLRWB before day 14, especially for critically ill patients. The difference of the basal hemolysis (day 0) percentages observed between subjects suggests that more studies should be performed to confirm a possible inter-individual donor biological variability of RBC membrane resistance, as happens in humans.

Glucose and Adenosine Triphosphate Level in Normal Subjects

1974 ◽  
Vol 125 (588) ◽  
pp. 459-460 ◽  
Author(s):  
J. Damas Mora ◽  
D. Vlissides ◽  
F. A. Jenner
Keyword(s):  
Blood Glucose ◽  
Correlation Coefficient ◽  
Adenosine Triphosphate ◽  
Whole Blood ◽  
Normal Subjects ◽  
Red Cell ◽  
Linus Pauling

In Orthomolecular Psychiatry; Treatment of Schizophrenia, edited by David Hawkins and Linus Pauling (1973), Beebe and Wendel (pp. 278–302) report a high correlation coefficient of r = 0.99 (which we calculate gives N = 42, p very much lower than 0.001) between whole blood glucose and adenosine triphosphate (ATP). This relationship they claim is no longer maintained in schizophrenics with anxiety, r = 0.16 (N = 62, p > 0.1). Erban and Hanzlicek (1966), Hansen (1972) and Hansen and Dimitrakoudi (1974) have suggested a possible significance of whole blood ATP in psychoses, and Naylor, Dick, Dick, Le Poidevin and Whyte (1973) have implicated red cell Na/K ATPases. The mechanisms involved in controlling blood ATP seemed therefore worthy of study especially if they are so dependent on glucose.

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