Assessment of the contamination from devices used for sampling and storage of whole blood and serum for element analysis

2001 ◽  
Vol 15 (1) ◽  
pp. 40-45 ◽  
Author(s):  
I. Rodushkin ◽  
F. Ödman
Keyword(s):  
Whole Blood ◽  
Element Analysis ◽  
And Storage

scholarly journals Effect of Leukoreduction on Hematobiochemical Parameters and Storage Hemolysis in Canine Whole Blood Units

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 925
Author(s):  
Maria Teresa Antognoni ◽  
Maria Luisa Marenzoni ◽  
Ambra Lisa Misia ◽  
Luca Avellini ◽  
Elisabetta Chiaradia ◽  
...  
Keyword(s):  
Whole Blood ◽  
Complete Blood Count ◽  
Membrane Resistance ◽  
Progressive Increase ◽  
Rbc Membrane ◽  
Morphological Index ◽  
The Difference ◽  
Hematobiochemical Parameters ◽  
And Storage ◽  
Individual Donor

Storage lesions (SLs) occur when the red blood cell quality is altered during the preservation of blood units. Pre-storage leukoreduction would limit the number of SLs. The aims of this study were to evaluate the effectiveness of a leukoreduction filter for human use and the effect of pre-storage leukoreduction on some ematobiochemical parameters in stored canine whole blood. Seven canine blood units were tested. Each one was divided into two units—one leukoreduced (LRWB) and one non-leukoreduced (nLRWB). On each unit, we determined the complete blood count (CBC), lactate-dehydrogenase (LDH), electrolytes (Na+, K+, Cl−), morphological index (MI) and hemolysis, on storage days 0, 7, 14, 21, 28, 35, and 42. Leukoreduction allowed a 98.30% recovery of the RBC count, retaining 99.69% and 94.91% of WBCs and PLTs, respectively. We detected a significant increase of LDH and MI with strongly higher values in nLRWB compared to LRWB. A progressive increase in electrolytes and LDH concentrations was observed as indices of stored hemolysis. LDH showed significantly lower values in LRWB units compared to nLRWB, suggesting its release from leukocytes. In the majority of units, hemolysis reached 1% on the 42nd day of storage. We assert the human leukoreduction filter effectiveness on canine whole blood, and we recommend using nLRWB before day 14, especially for critically ill patients. The difference of the basal hemolysis (day 0) percentages observed between subjects suggests that more studies should be performed to confirm a possible inter-individual donor biological variability of RBC membrane resistance, as happens in humans.

Should phosphatidylethanol be currently analysed using whole blood, dried blood spots or both?

2019 ◽  
Vol 57 (5) ◽  
pp. 617-622 ◽  
Author(s):  
Van Long Nguyen ◽  
Michael Fitzpatrick
Keyword(s):  
Whole Blood ◽  
Blood Cells ◽  
Dried Blood Spots ◽  
Storage Conditions ◽  
Ethanol Metabolism ◽  
Clinical Use ◽  
Blood Spots ◽  
Comparative Review ◽  
And Storage

Abstract Phosphatidylethanol (PEth) are phospholipids produced through non-oxidative ethanol metabolism. They accumulate in red blood cells and have been traditionally analysed in whole blood as potential biomarkers for moderate to long-term alcohol consumption. More recently, their analysis in dried blood spots has been gaining favour, namely, due to the ease in sampling, transport and storage conditions required. This paper aims at providing a short comparative review between analysing PEth in whole blood and dried blood spots and the potential pitfalls that researchers may face when setting up PEth testing for clinical use.

PRODUCTION OF PLATELETS FOR TRANSFUSION - CURRENT METHODS AND PROBLEMS TO BE SOLVED

1987 ◽  
Author(s):  
M K Elias ◽  
C Th Smit Sibinga
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Synthetic Medium ◽  
Buffy Coat ◽  
Cotton Wool ◽  
Single Donor ◽  
Increased Risk ◽  
Multiple Donors ◽  
And Storage ◽  
Selection Of

Initially, whole blood or platelet rich plasma were used as sources of platelets. Nowadays the methods of platelet concentrates (pc) production adopted in Blood Banks include the traditional method of platelet preparation by differential centrifugation of units of whole blood, besides the much more sophisticated technique of extracorporeal collection of pc with improved immunological compatibility.Manually pc are produced by the platelet rich plasmamethod, the buffy coat method and multiple bag plateletapheresis. The machine collection of pc is done by plateletapheresis or platelet elutriation, with different degrees of automation.The standard manual method remains quantitatively the most important source of platelets.However, there are major concerns:-the need of multiple donors-The high contamination with white cells, predominantly lymphocytes-these pc are depleted from larger and more active platelets, as these are sedimented with the red cells-increased risk of bacterial contamination. To solve these problems there are some potention solutions:-use of single donor collectioon techniques-depletion of leucocytes by:a.elutriation of platelets from the buffy coatb.filtration of random pc through cotton wool columnc. prostacyclin inhibition of platelet aggregation followed by cellulose acetate filtrationd.filtration on elutriated platelets through cotton wool-use of a platelet synthetic medium void of glucose for resuspension and storage of pc to prevent lactate accumulation and pH fall-use of closed sterile harness systems to collect platelets by surge plateletapheresis, which allows extended storage of leucocyte depleted pc.Selection of the most appropriate platelet concentrate depends on the interrelationship of many factors:1) yield 2) function 3) viability after storage 4) afety 5) purity 6) potency 7) efficacy (recovery, survival and haemostatic capacity).

scholarly journals Analysis of whole blood by ICP-MS equipped with a high temperature total sample consumption system

2017 ◽  
Vol 32 (1) ◽  
pp. 78-87 ◽  
Author(s):  
Águeda Cañabate ◽  
Esperanza García-Ruiz ◽  
Martín Resano ◽  
José-Luis Todolí
Keyword(s):  
High Temperature ◽  
Whole Blood ◽  
Inductively Coupled Plasma ◽  
Total Sample ◽  
Sample Introduction ◽  
Element Analysis ◽  
Inductively Coupled ◽  
System P ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

In this work, the performance of a high temperature torch Integrated Sample Introduction System (hTISIS) using inductively coupled plasma mass spectrometry (ICP-MS) for the multi-element analysis of whole blood is evaluated.

Impact of Alloantigens and Storage-associated Factors on Stimulated Cytokine Response in an In Vitro  Model of Blood Transfusion

2002 ◽  
Vol 97 (5) ◽  
pp. 1102-1109 ◽  
Author(s):  
Andreas E. Biedler ◽  
Sven O. Schneider ◽  
Ullrich Seyfert ◽  
Hauke Rensing ◽  
Sasha Grenner ◽  
...  
Keyword(s):  
Whole Blood ◽  
Mononuclear Cells ◽  
Autologous Blood ◽  
Immunosuppressive Effect ◽  
Tnf Alpha ◽  
Major Surgery ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fresh Blood ◽  
The Impact ◽  
And Storage

Background Transfusion of blood may contribute to immunosuppression in major surgery. The authors assessed the impact of alloantigens and storage on function of peripheral blood mononuclear cells cultured in their physiologic environment. Methods Blood units (whole blood, packed erythrocytes) were prepared with or without prestorage leukodepletion and stored for 24-26 days. Blood samples were coincubated with allogeneic fresh blood, autologous, or allogeneic stored blood. Endotoxin-stimulated release of tumor necrosis factor-alpha (TNF-alpha) and interleukin 10 (IL-10) was measured after 24 h of culture by enzyme-linked immunosorbent assay. Results Coincubation with equal amounts of allogeneic fresh blood showed almost no influence on TNF-alpha (-12%, not significant) and IL-10 (+11%, not significant) release. Stored allogeneic whole blood resulted in a significant TNF-alpha depression (-61%) and IL-10 induction (+221%). These effects were diminished but not prevented by prestorage leukodepletion (TNF-alpha -42%, IL-10 +110%) and required the presence of soluble factors (TNF-alpha suppression) and cellular components (IL-10 induction). TNF-alpha decrease and IL-10 increase were in the same order of magnitude (-40%, +134% with, -65%, +314% without leukodepletion) after coincubation with autologous blood. In contrast, allogeneic erythrocytes had only little effects (TNF-alpha -6%, IL-10 +36%) even at this high transfusion equivalent. Conclusion These data suggest that banked whole blood has an immunosuppressive effect that is largely attributable to storage-dependent factors. These factors are partially removed by prestorage leukodepletion, while the contribution of alloantigens is of minor significance. Immunosuppressive effects are least apparent with leukodepleted erythrocytes, suggesting that the presence of plasma during storage is required for the immunosuppressive effect to develop.

Generation of kinins during preparation and storage of whole blood?derived platelet concentrates

Transfusion ◽  
2007 ◽  
Vol 47 (3) ◽  
pp. 410-420 ◽  
Author(s):  
Marie Eve Moreau ◽  
Louis Thibault ◽  
Anik Désormeaux ◽  
Miguel Chagnon ◽  
Réal Lemieux ◽  
...  
Keyword(s):  
Whole Blood ◽  
Platelet Concentrates ◽  
And Storage

Citrate Storage Affects Thrombelastograph®Analysis

2000 ◽  
Vol 92 (5) ◽  
pp. 1242-1249 ◽  
Author(s):  
Vladimir Camenzind ◽  
Thomas Bombeli ◽  
Burkhardt Seifert ◽  
Marina Jamnicki ◽  
Dragoljub Popovic ◽  
...  
Keyword(s):  
Blood Coagulation ◽  
Analysis Of Variance ◽  
Whole Blood ◽  
Repeated Measures ◽  
Surgical Patients ◽  
Time Points ◽  
Prothrombin Fragment ◽  
Repeated Measures Analysis ◽  
Thrombin Activation ◽  
And Storage

Background Thrombelastograph analysis (TEG) is used to evaluate blood coagulation. Ideally, whole blood is immediately processed. If impossible, blood may be citrated and assessed after recalcification. No data describe the effect of such treatment and storage on TEG parameters. Methods Three studies were performed in 90 surgical patients. In 30 patients, blood was citrated (1:10, 0. 129 M) and recalcified (20 microl 2 M CaCl2 to 340 microl citrated blood), and TEG was performed with native blood and after recalcification after 0, 15, and 30 min of citrate storage. In another 30 patients, TEG was performed with citrated blood recalcified immediately and after 1-72 h storage. In a third study, thrombin-antithrombin complex, prothrombin fragment 1+2, and beta-thromboglobulin were measured (using enzyme-linked immunoabsorbant assay tests) at corresponding time points. Data were compared using repeated-measures analysis of variance and post hocpaired t tests. Results TEG parameters were different in recalcified citrated blood compared with native blood (P < 0.05) and changed significantly during 30-min (P < 0.025) and 72-h (P < 0.001) citrate storage. TEG parameters measured between 1 and 8 h of citrate storage were stable. Thrombin-antithrombin complex and prothrombin fragment 1+2 values were not elevated in native blood. After 30 min of citrate storage a gradual thrombin activation was observed, as evidenced by increasing thrombin-antithrombin complex and prothrombin fragment 1+2 values (P < 0.05). beta-Thromboglobulin level was increased after 2 and 8 h of citrate storage (P < 0.01). Conclusions Analysis of native blood yields the most reliable TEG results. Should immediate TEG processing not be possible, citrated blood may be used if recalcified after 1-8 h.

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