Histomorphological findings and expression of matrix metalloproteinases and their tissue specific inhibitors (TIMPs) in normal tricuspid valves and in chronic tricuspid valvular disease in dogs

Cattle undergo numerous environmental and management stressors that reduce fertility and affect ovulation. The extracellular matrix of the follicle wall can be altered by matrix metalloproteinases (MMPs), the activities of which are regulated by interleukins and tissue-specific inhibitors of metalloproteinases (TIMPs), especially during ovulation. The aims of the present study were to: (1) evaluate changes in the hormone milieu, the localisation and activity of MMP2 and MMP9 and the localisation of MMP14, TIMP1 and TIMP2 in response to adrenocorticotrophic hormone (ACTH) during the preovulatory period in cows; and (2) determine the direct effects of ACTH on the mRNA expression of MMP2 and MMP9 in the cultured follicle wall of bovine ovaries obtained from an abattoir. 100IU ACTH was administered during pro-oestrus every 12h until ovariectomy, which was performed before ovulation. Cortisol concentrations in the plasma and follicular fluid (FF) of preovulatory follicles were higher in ACTH-treated than control cows. Progesterone presented subluteal concentrations in plasma of ACTH-treated cows (P<0.05). MMP2 immunostaining and activity in ovaries were higher in ACTH-treated than control cows (P<0.05), whereas MMP9 immunostaining was similar between the two groups. However, unlike in control cows, MMP9 activity was absent in the FF of ACTH-treated cows. These results suggest that the administration of ACTH during the preovulatory period in cows could cause changes that culminate in modifications in the content and activation of MMPs and TIMPs in the ovary, which could interfere with the ovulation process.

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An immunohistochemical study of the role of matrix metalloproteinases and their tissue inhibitors in chronic mitral valvular disease (valvular endocardiosis) in dogs

The Veterinary Journal ◽

10.1016/j.tvjl.2007.11.011 ◽

2009 ◽

Vol 180 (1) ◽

pp. 88-94 ◽

Cited By ~ 20

Author(s):

Heike Aupperle ◽

Jens Thielebein ◽

Birgit Kiefer ◽

Imke März ◽

Gregor Dinges ◽

...

Keyword(s):

Matrix Metalloproteinases ◽

Immunohistochemical Study ◽

Valvular Disease ◽

Tissue Inhibitors

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Expression of Matrix Metalloproteinases and Their Specific Inhibitors in Normal and Different Human Thyroid Tumor Cell Lines

Thyroid ◽

10.1089/thy.2004.14.881 ◽

2004 ◽

Vol 14 (11) ◽

pp. 881-888 ◽

Cited By ~ 31

Author(s):

E. Baldini ◽

M. Toller ◽

F.M. Graziano ◽

F.P. Russo ◽

M. Pepe ◽

...

Keyword(s):

Matrix Metalloproteinases ◽

Tumor Cell ◽

Cell Lines ◽

Thyroid Tumor ◽

Human Thyroid ◽

Tumor Cell Lines ◽

Specific Inhibitors

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SCI1, the first member of the tissue-specific inhibitors of CDK (TIC) class, is probably connected to the auxin signaling pathway

Plant Signaling & Behavior ◽

10.4161/psb.7.1.18525 ◽

2012 ◽

Vol 7 (1) ◽

pp. 53-58 ◽

Cited By ~ 2

Author(s):

Henrique DePaoli ◽

Gustavo Goldman ◽

Maria-Helena Goldman

Keyword(s):

Signaling Pathway ◽

Auxin Signaling ◽

Tissue Specific ◽

Auxin Signaling Pathway ◽

Specific Inhibitors

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Analysis of Acetylcholinesterase Activity in Cell Membrane Microarrays of Brain Areas as a Screening Tool to Identify Tissue Specific Inhibitors

Analytica ◽

10.3390/analytica2010003 ◽

2021 ◽

Vol 2 (1) ◽

pp. 25-36

Author(s):

Bárbara Rienda ◽

Ane Elexpe ◽

Tarson Tolentino-Cortez ◽

Marina Gulak ◽

Cristina Bruzos-Cidón ◽

...

Keyword(s):

Cell Membrane ◽

High Throughput Screening ◽

Ache Activity ◽

Screening Tool ◽

Wide Spectrum ◽

Acetylcholinesterase Activity ◽

Brain Areas ◽

Tissue Specific ◽

Ache Inhibitors ◽

Specific Inhibitors

Acetylcholinesterase (AChE) is responsible for hydrolyzing the acetylcholine neurotransmitter, bringing an end point to cholinergic neurotransmission. Thus, AChE is the primary target of a wide spectrum of compounds used as pesticides, nerve agents or therapeutic drugs for neurodegenerative diseases such as Alzheimer’s disease (AD). This enzyme is heterogeneously distributed in the brain showing different activity depending on the nervous region. Therefore, the aim of this work is to report a novel technology that enables the simultaneous determination of tissue specific AChE activity, as well as the analysis and screening of specific inhibitors, by using cell membrane microarrays. These microarrays were composed of cell membranes, isolated from 41 tissues, organs and brain areas, that were immobilized over a slide, maintaining the functionality of membrane proteins. To validate this platform, demonstrating its usefulness in drug discovery as a high throughput screening tool, a colorimetric protocol to detect the membrane-bound AChE activity was optimized. Thus, rat cortical and striatal AChE activities were estimated in presence of increased concentrations of AChE inhibitors, and the donepezil effect was assessed simultaneously in 41 tissues and organs, demonstrating the major potential of this microarray’s technology.

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