Adoptive transfer of protective immunity from Cryptosporidium parvum-infected interferon-γ and interleukin-12-deficient mice to naive recipients

IFNγ receptor (IFNγR) deficient mice and IL-4 deficient mice were infected with blood-stage Plasmodium chabaudi AS in order to analyse the role of Th1 (IFNγ) and Th2 (IL-4)-associated cytokines in the development of protective immunity to the parasite. A high mortality rate and failure to reduce the primary parasitaemia to subpatent levels was observed in the IFNγR deficient mice. IL-4 deficient mice controlled a primary P. chabaudi AS infection in a similar manner to control mice and no mortality was observed. IFNγR deficient mice had a reduction in parasite-specific IgG and a significantly increased level of total IgE compared to control mice. There was no reduction in the level of parasite-specific IgG in IL-4 deficient mice. Cytological analysis of the cells present in the spleen and liver during the primary parasitaemia revealed a reduction in the numbers of lymphocytes, monocytes and polymorphonuclear (PMN) cells in the liver at the peak of parasitaemia in both IFNγR deficient mice and IL-4 deficient mice compared to control mice. Adoptive transfer studies demonstrated that cells isolated from the liver at day 11 post-infection could confer some protective immunity to P. chabaudi AS infection.

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Human Interleukin-12-Interferon-γ Axis in Protective Immunity to Mycobacteria

Cytokines and Chemokines in Infectious Diseases Handbook ◽

10.1385/1-59259-309-7:151 ◽

2003 ◽

pp. 151-161

Author(s):

Claire Fieschi ◽

Stéphanie Dupuis ◽

Capucine Picard ◽

Jean-Laurent Casanova

Keyword(s):

Protective Immunity ◽

Interferon Γ ◽

Interleukin 12

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Anamnestic Protective Immunity to Bacillus anthracis Is Antibody Mediated but Independent of Complement and Fc Receptors

Infection and Immunity ◽

10.1128/iai.00647-07 ◽

2008 ◽

Vol 76 (5) ◽

pp. 2177-2182 ◽

Cited By ~ 6

Author(s):

Eric T. Harvill ◽

Manuel Osorio ◽

Crystal L. Loving ◽

Gloria M. Lee ◽

Vanessa K. Kelly ◽

...

Keyword(s):

T Cell ◽

Bacillus Anthracis ◽

Adoptive Transfer ◽

Protective Immunity ◽

Fc Receptors ◽

Immunodeficient Mice ◽

Antibody Titers ◽

Immunocompetent Mice ◽

Induced Immunity ◽

Deficient Mice

ABSTRACT The threat of bioterrorist use of Bacillus anthracis has focused urgent attention on the efficacy and mechanisms of protective immunity induced by available vaccines. However, the mechanisms of infection-induced immunity have been less well studied and defined. We used a combination of complement depletion along with immunodeficient mice and adoptive transfer approaches to determine the mechanisms of infection-induced protective immunity to B. anthracis. B- or T-cell-deficient mice lacked the complete anamnestic protection observed in immunocompetent mice. In addition, T-cell-deficient mice generated poor antibody titers but were protected by the adoptive transfer of serum from B. anthracis-challenged mice. Adoptively transferred sera were protective in mice lacking complement, Fc receptors, or both, suggesting that they operate independent of these effectors. Together, these results indicate that antibody-mediated neutralization provides significant protection in B. anthracis infection-induced immunity.

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Enhanced Sensitivity of Tumor Necrosis Factor/Lymphotoxin‐α–Deficient Mice toCryptococcus neoformansInfection despite Increased Levels of Nitrite/Nitrate, Interferon‐γ, and Interleukin‐12

The Journal of Infectious Diseases ◽

10.1086/315061 ◽

1999 ◽

Vol 180 (5) ◽

pp. 1637-1647 ◽

Cited By ~ 23

Author(s):

Naïma Rayhane ◽

Olivier Lortholary ◽

Catherine Fitting ◽

Jacques Callebert ◽

Michel Huerre ◽

...

Keyword(s):

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Interferon Γ ◽

Interleukin 12 ◽

Enhanced Sensitivity ◽

Nitrite Nitrate ◽

Lymphotoxin Α ◽

Deficient Mice ◽

Necrosis Factor

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A dendritic cell vaccine against invasive aspergillosis in allogeneic hematopoietic transplantation

Blood ◽

10.1182/blood-2003-03-0748 ◽

2003 ◽

Vol 102 (10) ◽

pp. 3807-3814 ◽

Cited By ~ 168

Author(s):

Silvia Bozza ◽

Katia Perruccio ◽

Claudia Montagnoli ◽

Roberta Gaziano ◽

Silvia Bellocchio ◽

...

Keyword(s):

Adoptive Transfer ◽

Dendritic Cell Vaccine ◽

Interferon Γ ◽

Lymphoid Cells ◽

Interleukin 12 ◽

Cell Vaccine ◽

Allogeneic Bone ◽

Hematopoietic Transplantation

AbstractDendritic cells (DCs) have a remarkable functional plasticity in response to conidia and hyphae of the fungus Aspergillus fumigatus. In the present study we sought to assess the capacity of DCs activated by live fungi or fungal RNA to generate antifungal immunity in vivo. We found that both human and murine DCs pulsed with live fungi or transfected with fungal RNA underwent functional maturation, as revealed by the up-regulated expression of histocompatibility class II antigen and costimulatory molecules and the production of interleukin 12 (IL-12) in response to conidia or conidial RNA and of IL-4/IL-10 in response to hyphae or hyphal RNA. DCs pulsed with conidia or transfected with conidial RNA activated antigen-specific, interferon γ (IFN-γ)-producing T lymphocytes in vitro and in vivo on adoptive transfer in mice otherwise susceptible to aspergillosis. TH1-dependent antifungal resistance could also be induced in mice receiving allogeneic bone marrow transplants and was associated with an accelerated recovery of myeloid and lymphoid cells. Because the efficacy of the infusion of DCs was superior to that obtained on the adoptive transfer of Aspergillus-specific T cells, these results indicate the vaccinating potential of DCs pulsed with Aspergillus conidia or conidial RNA in hematopoietic transplantation. (Blood. 2003;102:3807-3814)

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