Rh1 high activity binding peptides inhibit high percentages of Plasmodium falciparum FVO strain invasion

Solid experimental evidence indicates that EBA-175 is used as a ligand by the Plasmodium falciparum merozoite to bind to human RBC, via different binding processing fragments. Using synthetic peptides and specific receptor-ligand interaction methodology, we have identified 6 high-activity binding sequences from the EBA-175 CAMP strain; peptide 1758 (KSYGTPDNIDKNMSLIHKHN), located in the so-called region I for which no binding activity has been reported before, peptides 1779 (NIDRIYDKNLLMIKEHILAI) and 1783 (HRNKKNDKLYRDEWWKVIKK), located in region II, in a sub-region known as 5′ Cys F2, previously reported as being a binding region, and peptides 1814 (DRNSNTLHLKDYRNEENERH), 1815 (YTNQNINISQERDLQKHGFH) and 1818 (NNNFNNIPSRYNLYDKKLDL), in region III–V where antibodies inhibit merozoite invasion of erythrocytes. The affinity constants were between 60 and 180 nM and the critical amino acids involved in the binding were identified. The binding of these peptides to enzyme-treated RBC was analysed; binding of peptide 1814, located in the III–V region, was found to be sialic acid dependent. Some of these high binding peptides were able to inhibit in vitro merozoite invasion and to block the binding of recombinant RII-EBA to RBC. Several of these peptides are located in regions recognized by protective immune clusters of merozoites (ICMs) eluted antibodies.

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Conserved High Activity Binding Peptides are Involved in Adhesion of Two Detergent-Resistant Membrane-Associated Merozoite Proteins to Red Blood Cells during Invasion

Journal of Medicinal Chemistry ◽

10.1021/jm901474p ◽

2010 ◽

Vol 53 (10) ◽

pp. 3907-3918 ◽

Cited By ~ 9

Author(s):

Ana Zuleima Obando-Martinez ◽

Hernando Curtidor ◽

Gabriela Arévalo-Pinzón ◽

Magnolia Vanegas ◽

Carolina Vizcaino ◽

...

Keyword(s):

Red Blood Cells ◽

High Activity ◽

Blood Cells ◽

Binding Peptides ◽

Detergent Resistant Membrane

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Aktivitas Antimalaria Senyawa Flavanon Terisoprenilasi Dari Kulit Batang Erythrina fusca L

Jurnal Pharmascience ◽

10.20527/jps.v5i1.5786 ◽

2018 ◽

Vol 5 (1) ◽

Author(s):

Novi Fatmawati ◽

Novi Anggreini ◽

Ratih Dewi Saputri ◽

Tjitjik Srie Tjahjandarie ◽

Mulyadi Tanjung

Keyword(s):

Plasmodium Falciparum ◽

High Activity ◽

Spectroscopic Data ◽

Stem Bark ◽

Falciparum Strain ◽

Very High

ABSTRAK Dua senyawa flavanon terisoprenilasi yakni lonkokarpol A (1) dan lupinifolin (2) telah diisolasi dari kulit batang Erythrina fusca L. Struktur senyawa flavanon terisoprenilasi ditetapkan berdasarkan analisis spektroskopi UV, IR, MS dan NMR. Uji aktivitas antimalarial senyawa 1–2 terhadap Plasmodium falciparum strain 3D7 memperlihatkan IC50 1,18 dan 0,82 µg/ml yang dikategorikan sangat aktif. Kata kunci : Erythrina fusca L., lonkokarpol A, lupinifolin, antimalaria ABSTRACT Two isoprenylated flavanones namely as lonchocarpol A (1) dan lupinifolin (2) were isolated from the stem bark of Limonia accidissima L. Erythrina fusca L. Their structures were determined based on spectroscopic data such as UV, IR, MS and NMR. Compounds 1–2 were evaluated for their antimalarial against Plasmodium falciparum strain 3D7 showing their IC50 were 1.18 and 0.8 µg/ml, respectively and very high activity. Keywords : Erythrina fusca L., lonchocarpol A, lupinifolin, antimalarial

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Mycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasion

Peptides ◽

10.1016/j.peptides.2012.08.023 ◽

2012 ◽

Vol 38 (2) ◽

pp. 208-216 ◽

Cited By ~ 6

Author(s):

Diana Marcela Rodríguez ◽

Marisol Ocampo ◽

Hernando Curtidor ◽

Magnolia Vanegas ◽

Manuel Elkin Patarroyo ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

High Activity ◽

Cell Invasion ◽

Surface Protein ◽

Binding Peptides

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Selection of T cell epitopes on pre-erythrocytic Plasmodium falciparum antigens by artificial neural networks: validation of HLA class I binding peptides as T cell epitopes in an endemic area of Papua New Guinea.

Parasitology International ◽

10.1016/s1383-5769(98)80133-5 ◽

1998 ◽

Vol 47 ◽

pp. 73

Author(s):

J.W. Kazura ◽

K Bucci ◽

V Brusic ◽

C.L. King ◽

M.P. Alpers

Keyword(s):

Neural Networks ◽

Plasmodium Falciparum ◽

Artificial Neural Networks ◽

T Cell ◽

Papua New Guinea ◽

Endemic Area ◽

Hla Class I ◽

T Cell Epitopes ◽

Binding Peptides ◽

Selection Of

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AKTIVITAS ANTIMALARIA SENYAWA FLAVANON TERISOPRENILASI DARI KULIT BATANG Erythrina fusca L.

JURNAL KIMIA MULAWARMAN ◽

10.30872/jkm.v16i1.586 ◽

2018 ◽

Vol 16 (1) ◽

pp. 40

Author(s):

Novi Fatmawati ◽

Novi Anggreini ◽

Ratih Dewi Saputri ◽

Tjitjik Srie Tjahjandarie ◽

Mulyadi Tanjung

Keyword(s):

Plasmodium Falciparum ◽

High Activity ◽

Spectroscopic Data ◽

Stem Bark ◽

Falciparum Strain ◽

Very High

Two isoprenylated flavanones namely as lonchocarpol A (1) dan lupinifolin (2) were isolated from the stem bark of Limonia accidissima L. Erythrina fusca L. Their structures were determined based on spectroscopic data such as UV, IR, MS and NMR. Compounds 1–2 were evaluated for their antimalarial against Plasmodium falciparum strain 3D7 showing their IC50 were 1.18 and 0.8 µg/ml, respectively and very high activity.

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