Reversible synthesis of colanic acid and O-antigen polysaccharides in Salmonella Typhimurium enhances induction of cross-immune responses and provides protection against heterologous Salmonella challenge

Vaccine ◽  
2017 ◽  
Vol 35 (21) ◽  
pp. 2862-2869 ◽  
Author(s):  
Pei Li ◽  
Qing Liu ◽  
Chun Huang ◽  
Xinxin Zhao ◽  
Kenneth L. Roland ◽  
...  
Keyword(s):  
Immune Responses ◽  
Salmonella Typhimurium ◽  
O Antigen ◽  
Colanic Acid

scholarly journals Energy dependence of O-antigen synthesis in Salmonella typhimurium.

1991 ◽  
Vol 173 (10) ◽  
pp. 3128-3133 ◽  
Author(s):  
P A Marino ◽  
B C McGrath ◽  
M J Osborn
Keyword(s):  
Salmonella Typhimurium ◽  
Energy Dependence ◽  
O Antigen

Construction and immunological assessment of Salmonella typhimurium expressing fox sperm LDH-C4

1998 ◽  
Vol 10 (3) ◽  
pp. 225 ◽  
Author(s):  
Peter Bird ◽  
Christine Hayes ◽  
James de Jersey ◽  
Mark Bradley
Keyword(s):  
Immune Responses ◽  
Salmonella Typhimurium ◽  
Oral Vaccine ◽  
Red Foxes ◽  
Expression Plasmid ◽  
Iga Response ◽  
Specific Igg ◽  
The Common ◽  
Oral Doses ◽  
Specific Igg Antibodies

This study examined immune responses of foxes to oral doses of recombinant Salmonella typhimurium expressing fox sperm-specific lactate dehydrogenase (fLDH). The cDNA for fLDH was cloned into the expression plasmid pKK233· 2 (pKKfLDH). Salmonella typhimuriumaroA– (SL3261) was transformed with either the pKK233·2 plasmid alone (SpKK) or the pKKfLDH construct (SpKfLDH). The fLDH expressed by SpKfLDH retained enzymatic activity and was recognized by human LDH-C4-specific antibody. Male European red foxes (Vulpes vulpes) were given an initial oral dose of 1 × 1011 cfu of either SpKK (control, n = 3) or SpKfLDH (test, n = 6), followed four weeks later with a further dose of 1 × 1011 cfu. Antibodies to Salmonella lipopolysaccharide (LPS-04) and fLDH were measured in plasma and saliva for eight consecutive weeks after the initial doses. Both LPS-04 IgG- and IgA-specific antibodies as well as fLDH-specific IgG antibodies were detected in plasma and saliva. However, there was a marked fLDH-specific IgA response in saliva consistent with induction of the common mucosal immune system. The antibody measurements demonstrated the feasibility of using recombinant Salmonella as an oral vaccine to elicit gamete antigen-specific mucosal immune responses in foxes.

Sign in / Sign up

Export Citation Format

Share Document