H9c2 cell line is a valuable in vitro model to study the drug metabolizing enzymes in the heart

The aim of this study was to investigate the endpoints related to cell death, either necrosis or apoptosis, induced by four chemicals in the promyelocytic leukemia cell line, HL-60. Cell morphology, DNA fragmentation, cytofluorimetric analysis and oxygen consumption were used to classify the type of cell death observed. In our analysis, we found that not all the selected parameters reproduced the differences observed in the cell death caused by the four chemicals tested. As cell death is a very complex phenomenon, several factors should be taken into account (cell type, exposure time and chemical concentration), if chemicals are to be classified according to differences in the mechanisms more directly involved in cell death.

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Homeopathic potencies of Arnica montana L. change gene expression in a Tamm-Horsfall protein-1 cell line in vitro model: the role of ethanol as a possible confounder and statistical bias

Journal of Integrative Medicine ◽

10.1016/s2095-4964(17)60346-7 ◽

2017 ◽

Vol 15 (4) ◽

pp. 255-264 ◽

Cited By ~ 4

Author(s):

Salvatore Chirumbolo ◽

Geir Bjørklund

Keyword(s):

Gene Expression ◽

Cell Line ◽

In Vitro Model ◽

Statistical Bias ◽

Arnica Montana ◽

Vitro Model

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An in vitro model for investigating intestinal adhesion of potential dairy propionibacteria probiotic strains using cell line C2BBe1

Letters in Applied Microbiology ◽

10.1046/j.1472-765x.2003.01303.x ◽

2003 ◽

Vol 36 (4) ◽

pp. 213-216 ◽

Cited By ~ 30

Author(s):

Y. Huang ◽

M.C. Adams

Keyword(s):

Cell Line ◽

In Vitro Model ◽

Intestinal Adhesion ◽

Probiotic Strains ◽

Dairy Propionibacteria ◽

Vitro Model

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Comparative Analysis of Antioxidant Behavior of Salacia Oblonga and Mangiferin in Tobacco Smoke Oxidatively Stressed In Vitro Model (L6 Cell Line)

Indo Global Journal of Pharmaceutical Sciences ◽

10.35652/igjps.2014.22 ◽

2014 ◽

Vol 04 (03) ◽

Author(s):

Sujata Basu ◽

Mamta Pant ◽

Rachana

Keyword(s):

Comparative Analysis ◽

Cell Line ◽

Tobacco Smoke ◽

In Vitro Model ◽

Salacia Oblonga ◽

Vitro Model

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Erratum to “Monolayers of IEC-18 cells as an in vitro model for screening the passive transcellular and paracellular transport across the intestinal barrier: comparison of active and passive transport with the human colon carcinoma Caco-2 cell line”

Environmental Toxicology and Pharmacology ◽

10.1016/s1382-6689(02)00123-0 ◽

2003 ◽

Vol 13 (1) ◽

pp. 55 ◽

Cited By ~ 5

Author(s):

Carolien H.M Versantvoort ◽

Rob C.A Ondrewater ◽

Erwin Duizer ◽

Johannes J.M Van de Sandt ◽

Andries J Gilde ◽

...

Keyword(s):

Cell Line ◽

Colon Carcinoma ◽

In Vitro Model ◽

Intestinal Barrier ◽

Human Colon ◽

Paracellular Transport ◽

Passive Transport ◽

Human Colon Carcinoma ◽

Vitro Model

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Cytoprotective potential of anti-ischemic drugs against chemotherapy-induced cardiotoxicity in H9c2 myoblast cell line

Acta Pharmaceutica ◽

10.2478/acph-2013-0038 ◽

2013 ◽

Vol 63 (4) ◽

pp. 493-503 ◽

Cited By ~ 4

Author(s):

Tiam Feridooni ◽

Chris Mac Donald ◽

Di Shao ◽

Pollen Yeung ◽

Remigius U. Agu

Keyword(s):

Cell Death ◽

Cell Line ◽

Cancer Drug ◽

H9c2 Cell ◽

Drug Induced ◽

Cardiac Model ◽

Myoblast Cell Line ◽

Myoblast Cell ◽

H9c2 Cell Line

Abstract To investigate potential prevention or attenuation of anti- cancer drug induced cardiotoxicity using anti-ischemic drugs, a rat myoblast (H9c2) cell line was used as our in vitro cardiac model. Irinotecan and doxorubicin were found to be cytotoxic for the H9c2 cell line with IC50 of 30.69 ± 6.20 and 20.94 ± 6.05 mmol L-1, respectively. 5-Flurouracil and cladribine were not cytotoxic and thus IC50 could not be calculated. When 100 mmol L-1 doxorubicin was incubated for 72 hours with 50 mmol L-1 diltiazem, 100 mmol L-1 dexrazoxane and 100 mmol L-1 losartan, respectively, there was a 58.7 ± 10.2, 52.2 ± 11.7 and 44.7 ± 5.4 % reduction in cell death. When 200 mmol L-1 irinotecan was incubated for 72 hours with 100 mmol L-1 dexrazoxane, losartan and diltiazem, respectively, a 27.7 ± 6.9, 25.6 ± 5.1, and 19.1 ± 2.3 % reduction in cell death was observed. Our data suggests that losartan and diltiazem were as effective as dexrazoxane in protecting the cells against irinotecan- and doxorubicin-induced cell toxicity. These findings offer potential uses of anti- -ischemic drugs for ablation of cytotoxicity in response to mitochondrial injury, thereby improving patient outcomes and reducing health-care costs.

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