Induced Pluripotent Stem Cell-Derived Cardiomyocytes: An In Vitro Model to Predict Tyrosine Kinase Inhibitor (TKI)-Induced Structural Cardiotoxicity

2017 ◽  
Vol 88 ◽  
pp. 243
Author(s):  
Xi Yang ◽  
Matthew White ◽  
Qiang Shi ◽  
William Mattes ◽  
Thomas Papoian
Keyword(s):  
Stem Cell ◽  
Tyrosine Kinase ◽  
Tyrosine Kinase Inhibitor ◽  
Pluripotent Stem Cell ◽  
In Vitro Model ◽  
Kinase Inhibitor ◽  
Induced Pluripotent Stem Cell ◽  
Vitro Model ◽  
Induced Pluripotent

scholarly journals Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Fuminori Sakurai ◽  
Seiji Mitani ◽  
Tatsuro Yamamoto ◽  
Kazuo Takayama ◽  
Masashi Tachibana ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hepatitis B Virus ◽  
Pluripotent Stem Cell ◽  
In Vitro Model ◽  
Induced Pluripotent Stem Cell ◽  
Hepatitis B Virus Infection ◽  
B Virus ◽  
Vitro Model ◽  
Induced Pluripotent

scholarly journals An in vitro model of hepatic steatosis using lipid loaded induced pluripotent stem cell derived hepatocyte like cells

2020 ◽  
Vol 7 (3) ◽  
pp. 135
Author(s):  
Hiraganahalli Bhaskar Deepak ◽  
Nellikalaya Shreekrishna ◽  
Zaheerbasha Sameermahmood ◽  
Niranjan Naranapur Anand ◽  
Raghotham Hulgi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hepatic Steatosis ◽  
Pluripotent Stem Cell ◽  
In Vitro Model ◽  
Induced Pluripotent Stem Cell ◽  
Vitro Model ◽  
Induced Pluripotent

scholarly journals In vitro model of ischemic heart failure using human induced pluripotent stem cell-derived cardiomyocytes

JCI Insight ◽  
2021 ◽  
Author(s):  
Justin Davis ◽  
Ahmad Chouman ◽  
Jeffery Creech ◽  
Andre Monteiro da Rocha ◽  
Daniela Ponce-Balbuena ◽  
...  
Keyword(s):  
Heart Failure ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
In Vitro Model ◽  
Induced Pluripotent Stem Cell ◽  
Ischemic Heart ◽  
Ischemic Heart Failure ◽  
Vitro Model ◽  
Induced Pluripotent

scholarly journals Characterization of Human Induced Pluripotent Stem Cell-Derived Hepatocytes with Mature Features and Potential for Modeling Metabolic Diseases

2020 ◽  
Vol 21 (2) ◽  
pp. 469
Author(s):  
Gustav Holmgren ◽  
Benjamin Ulfenborg ◽  
Annika Asplund ◽  
Karin Toet ◽  
Christian X Andersson ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
In Vitro Model ◽  
Metabolic Diseases ◽  
Induced Pluripotent Stem Cell ◽  
Functional Assays ◽  
Vitro Model ◽  
Human Primary Hepatocytes ◽  
Induced Pluripotent

There is a strong anticipated future for human induced pluripotent stem cell-derived hepatocytes (hiPS-HEP), but so far, their use has been limited due to insufficient functionality. We investigated the potential of hiPS-HEP as an in vitro model for metabolic diseases by combining transcriptomics with multiple functional assays. The transcriptomics analysis revealed that 86% of the genes were expressed at similar levels in hiPS-HEP as in human primary hepatocytes (hphep). Adult characteristics of the hiPS-HEP were confirmed by the presence of important hepatocyte features, e.g., Albumin secretion and expression of major drug metabolizing genes. Normal energy metabolism is crucial for modeling metabolic diseases, and both transcriptomics data and functional assays showed that hiPS-HEP were similar to hphep regarding uptake of glucose, low-density lipoproteins (LDL), and fatty acids. Importantly, the inflammatory state of the hiPS-HEP was low under standard conditions, but in response to lipid accumulation and ER stress the inflammation marker tumor necrosis factor α (TNFα) was upregulated. Furthermore, hiPS-HEP could be co-cultured with primary hepatic stellate cells both in 2D and in 3D spheroids, paving the way for using these co-cultures for modeling non-alcoholic steatohepatitis (NASH). Taken together, hiPS-HEP have the potential to serve as an in vitro model for metabolic diseases. Furthermore, differently expressed genes identified in this study can serve as targets for future improvements of the hiPS-HEP.

29 Human induced pluripotent stem cell-derived cardiomyocytes serve as in vitro model of cardiac hypertrophy

Heart ◽  
2011 ◽  
Vol 97 (20) ◽  
pp. e7-e7 ◽  
Author(s):  
G. Foldes ◽  
M. Mioulane ◽  
M. N. Chahine ◽  
M. D. Schneider ◽  
S. E. Harding
Keyword(s):  
Stem Cell ◽  
Cardiac Hypertrophy ◽  
Pluripotent Stem Cell ◽  
In Vitro Model ◽  
Induced Pluripotent Stem Cell ◽  
Vitro Model ◽  
Induced Pluripotent
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