Interactive effects of dexamethasone and opsonized Mycoplasma bovis on bovine neutrophil function in vitro

2018 ◽  
Vol 196 ◽  
pp. 18-21
Author(s):  
Hussain A. Alabdullah ◽  
Lawrence K. Fox ◽  
John M. Gay ◽  
George M. Barrington
Keyword(s):  
Neutrophil Function ◽  
Interactive Effects ◽  
Mycoplasma Bovis ◽  
Bovine Neutrophil

Effects of dexamethasone and Mycoplasma bovis on bovine neutrophil function in vitro

2015 ◽  
Vol 164 (1-2) ◽  
pp. 67-73 ◽  
Author(s):  
Hussain A. Alabdullah ◽  
Lawrence K. Fox ◽  
John M. Gay ◽  
George M. Barrington ◽  
Robert H. Mealey
Keyword(s):  
Neutrophil Function ◽  
Mycoplasma Bovis ◽  
Bovine Neutrophil

scholarly journals Importance and Antimicrobial Resistance of Mycoplasma bovis in Clinical Respiratory Disease in Feedlot Calves

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1470
Author(s):  
Ana García-Galán ◽  
Juan Seva ◽  
Ángel Gómez-Martín ◽  
Joaquín Ortega ◽  
Francisco Rodríguez ◽  
...  
Keyword(s):  
Respiratory Disease ◽  
Clinical Signs ◽  
Antimicrobial Treatment ◽  
Mycoplasma Bovis ◽  
Bacterial Disease ◽  
Feedlot Calves ◽  
Fixed Panel

Bovine respiratory disease (BRD) is an important viral and/or bacterial disease that mainly affects feedlot calves. The involvement of Mycoplasma bovis in BRD can lead to chronic pneumonia poorly responsive to antimicrobial treatment. Caseonecrotic bronchopneumonia is a pulmonary lesion typically associated with M. bovis. In Spain, M. bovis is widely distributed in the feedlots and circulating isolates are resistant to most antimicrobials in vitro. However, the role of this species in clinical respiratory disease of feedlot calves remains unknown. Furthermore, available data are relative to a fixed panel of antimicrobials commonly used to treat BRD, but not to the specific set of antimicrobials that have been used for treating each animal. This study examined 23 feedlot calves raised in southeast Spain (2016–2019) with clinical signs of respiratory disease unresponsive to treatment. The presence of M. bovis was investigated through bacteriology (culture and subsequent PCR), histopathology and immunohistochemistry. The pathogen was found in 86.9% (20/23) of the calves, mainly in the lungs (78.26%; 18/23). Immunohistochemistry revealed M. bovis antigens in 73.9% (17/23) of the calves in which caseonecrotic bronchopneumonia was the most frequent lesion (16/17). Minimum inhibitory concentration assays confirmed the resistance of a selection of 12 isolates to most of the antimicrobials specifically used for treating the animals in vivo. These results stress the importance of M. bovis in the BRD affecting feedlot calves in Spain.

scholarly journals The Effect of Prednisolone on Canine Neutrophil Function: In Vivo and in Vitro Studies

1998 ◽  
Vol 39 (2) ◽  
pp. 201-213
Author(s):  
G. Trowald-Wigh ◽  
L. Håkansson ◽  
A. Johannisson ◽  
L.E. Edqvist
Keyword(s):  
Neutrophil Function ◽  
In Vitro Studies

scholarly journals Malate content of picoliter samples of Raphanus sativus cytoplasm.

1991 ◽  
Vol 39 (4) ◽  
pp. 435-440 ◽  
Author(s):  
M J Bodson ◽  
W H Outlaw ◽  
S H Silvers
Keyword(s):  
Root Hair ◽  
Phosphoenolpyruvate Carboxylase ◽  
Raphanus Sativus ◽  
Organic Anion ◽  
Root Hairs ◽  
Interactive Effects ◽  
Plant Metabolism ◽  
Individual Root

Malate, which plays many essential roles in plant metabolism, is a potent in vitro inhibitor of the cytosolic enzyme phosphoenolpyruvate carboxylase (PEPC). Because PEPC activity leads to malate biosynthesis, malate is assumed to attenuate its own synthesis in situ. To test this hypothesis, we measured directly the malate content of picoliter samples of Raphanus root-hair cytoplasm using quantitative histochemical techniques. We also obtained an estimate for malate accumulation in these cells. These values were compared with the PEPC activity of individual root hairs (less than 2 ng). The results indicate that high cytoplasmic malate concentration does not severely inhibit PEPC in situ. We suggest that the focus for studies on the regulation of organic anion accumulation be on the interactive effects of malate and other PEPC effectors.

scholarly journals In vitro effect of fatty acids on human neutrophil function and death

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Tania Cristina Pithon‐Curi ◽  
Adriana Cristina Levada‐Pires ◽  
Thais Martins Lima ◽  
Elaine Hatanaka ◽  
Rui Curi
Keyword(s):  
Fatty Acids ◽  
Human Neutrophil ◽  
Neutrophil Function ◽  
Vitro Effect
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