Isolation and characterization of a low pathogenic duck hepatitis A virus 3 from South Korea

A novel virus was detected from diseased ducks and completely determined. The virus was shown to have a picornavirus-like genome layout. Interestingly, the genome contained a total of up to six 2As, including four 2As (2A1–2A4) each having an NPGP motif, an AIG1-like 2A5, and a parechovirus-like 2A6. The 5′UTR was predicted to possess a hepacivirus/pestivirus-like internal ribosome entry site (IRES). However, the subdomain IIIe consisted of a 3 nt stem and five unpaired bases, distinct from those found in all other HP-like IRESs. The virus was most closely related to duck hepatitis A virus, with amino acid identities of 37.7 %, 39 % and 43.7 % in the P1, P2 and P3 regions, respectively. Based on these investigations, together with phylogenetic analyses, the virus could be considered as the founding member of a novel picornavirus genus that we tentatively named ‘Aalivirus’, with ‘Aalivirus A’ as the type species.

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Morphogenesis of hepatitis A virus: isolation and characterization of subviral particles.

Journal of Virology ◽

10.1128/jvi.64.11.5284-5289.1990 ◽

1990 ◽

Vol 64 (11) ◽

pp. 5284-5289 ◽

Cited By ~ 54

Author(s):

D A Anderson ◽

B C Ross

Keyword(s):

Hepatitis A ◽

Virus Isolation ◽

Hepatitis A Virus ◽

Isolation And Characterization ◽

Subviral Particles

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Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam

The Journal of Infection in Developing Countries ◽

10.3855/jidc.7239 ◽

2016 ◽

Vol 10 (09) ◽

pp. 988-995 ◽

Cited By ~ 14

Author(s):

Huong Thi Thanh Doan ◽

Xuyen Thi Kim Le ◽

Roan Thi Do ◽

Chau Thi Minh Hoang ◽

Khue Thi Nguyen ◽

...

Keyword(s):

Hepatitis A ◽

Hepatitis A Virus ◽

Phylogenetic Analyses ◽

Hypervariable Region ◽

Genetic Characteristics ◽

Molecular Genotyping ◽

Duck Hepatitis ◽

Polymerase Chain ◽

Different Strains

Introduction: The aim of this study was to identify the genetic characteristics and molecular genotyping of duck hepatitis A virus (DHAV) isolated in Vietnam during 2009–2013. Methodology: Thirty duckling livers from outbreaks between 2009 and 2013 in seven provinces were collected and identified by polymerase chain reaction (PCR). Then, VP1 genes of eleven positive samples and two attenuated vaccine strains were sequenced and analyzed. Results: Genotypic and phylogenetic analyses indicated that the 13 Vietnamese isolates were classified into two genotypes, DHAV-1 and DHAV-3. The rate of identity and homology was 91%–100% between the 10 Vietnamese and 26 global strains of DHAV-3, and 92%–100% between 3 Vietnamese and 16 strains of DHAV-1. Between the DHAV-3 and DHAV-1 strains, the divergence reached 30%. At the C-terminal of VP1 for the different strains, a hypervariable region was observed, and notably, six of the Vietnamese DHAV-3 strains in this study showed four consistent differences (at positions T184M, Q200H, K207N, and K214R) within this group that were distinct from all other DHAV-3 strains. Conclusions: This is the first report of molecular characterization of DHAVs in Vietnam. At least two genotypes were identified, DHAV-1 and DHAV-3, with diversified clades within and between genotypes. DHAV-3 seemed to be dominant in Vietnam.

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Construction and characterization of an improved DNA-launched infectious clone of duck hepatitis a virus type 1

Virology Journal ◽

10.1186/s12985-017-0883-5 ◽

2017 ◽

Vol 14 (1) ◽

Cited By ~ 7

Author(s):

Junhao Chen ◽

Ruihua Zhang ◽

Shaoli Lin ◽

Pengfei Li ◽

Jingjing Lan ◽

...

Keyword(s):

Virus Type ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Infectious Clone ◽

Duck Hepatitis

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Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100102481 ◽

1988 ◽

Vol 46 ◽

pp. 80-81

Author(s):

Charles D. Humphrey ◽

E. H. Cook ◽

Karen A. McCaustland ◽

Daniel W. Bradley

Keyword(s):

Electron Microscopy ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Solid Phase ◽

Etiologic Agent ◽

World Health ◽

Health Concern ◽

Morphological Identification ◽

Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

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