Identification of ABC transporter genes conferring combined pleuromutilin–lincosamide–streptogramin A resistance in bovine methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci

Methicillin-resistant staphylococcus aureus (MRSA) is an extremely infectious hospital acquired bacterial pathogen often found in post-surgical patients globally. Early detection of such pathogens is a critical requirement to eliminate or reduce the incidence of antimicrobial resistance as well as for effective management of the disease. Despite the development of multiple biochemical, microbiological and nucleic acid amplification techniques (NAATs), conventional culture methods are widely used clinically owing to high variability between the methods, technical skills, and infrastructural needs. Further, multiple reports suggest a significant variation among diagnostic output for MRSA detection. This work attempts to probe the discordance among the diagnostic output of three commonly used methods while trying to understand the underlying cause of variability. MRSA detection on 217 clinical pus isolates was carried out using three different methods namely, conventional culture method, qPCR-based amplification, and a modern LAMP-based detection approach. Also, to confirm the presence of MRSA and distinguish from coagulase-negative staphylococci (CoNS), as well as to investigate the observed differences between qPCR and LAMP outputs, melt curve analysis was performed on discordant samples. LAMP-based MRSA detection was found to be the optimum method. In summary, this study evaluates the diagnostic efficiency of the different detection methods, while probing for possible explanations for the observed differences.

Download Full-text

New antimicrobial cystatin C-based peptide active against gram-positive bacterial pathogens, including methicillin-resistant Staphylococcus aureus and multiresistant coagulase-negative staphylococci

Apmis ◽

10.1111/j.1600-0463.2003.t01-1-apm1111110.x ◽

2003 ◽

Vol 111 (11) ◽

pp. 1004-1010 ◽

Cited By ~ 15

Author(s):

AFTAB JASIR ◽

FRANCISZEK KASPRZYKOWSKI ◽

REGINA KASPRZYKOWSKA ◽

VERONICA LINDSTRÖM ◽

CLAES SCHALÉN ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Cystatin C ◽

Methicillin Resistant Staphylococcus Aureus ◽

Bacterial Pathogens ◽

Coagulase Negative Staphylococci ◽

Gram Positive ◽

Methicillin Resistant

Download Full-text

In Vitro Activities of Noveltrans-3,5-Disubstituted Pyrrolidinylthio-1β-Methylcarbapenems with Potent Activities against Methicillin-Resistant Staphylococcus aureus andPseudomonas aeruginosa

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.44.3.489-495.2000 ◽

2000 ◽

Vol 44 (3) ◽

pp. 489-495 ◽

Cited By ~ 23

Author(s):

Rie Nagano ◽

Kaneyoshi Shibata ◽

Yuka Adachi ◽

Hideaki Imamura ◽

Terutaka Hashizume ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Side Chain ◽

The Novel ◽

Good Activity ◽

Penicillin Binding Protein ◽

Coagulase Negative Staphylococci ◽

Methicillin Resistant ◽

The Family

ABSTRACT The in vitro activities of the novel 1β-methylcarbapenems J-111,225, J-114,870, and J-114,871, which have a structurally unique side chain that consists of a trans-3,5-disubstituted 5-arylpyrrolidin-3-ylthio moiety at the C-2 position, were compared with those of reference antibiotics. Among isolates of both methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MRCoNS), 90% were inhibited by J-111,347 (prototype), J-111,225, J-114,870, and J-114,871 at concentrations of 2, 4, 4, and 4 μg/ml (MICs at which 90% of isolates are inhibited [MIC90s]), respectively, indicating that these agents were 32- to 64-fold more potent than imipenem, which has an MIC90 of 128 μg/ml. Although these drugs were less active in vitro than vancomycin, which had MIC90s of 1 and 2 μg/ml for MRSA and MRCoNS, respectively, the new carbapenems displayed better killing kinetics than vancomycin. The potent anti-MRSA activity was ascribed to the excellent affinities of the new carbapenems for penicillin-binding protein 2a of MRSA. Since the new carbapenems also exhibited good activity against gram-positive and -negative bacteria including clinically important pathogens such as penicillin-resistantStreptococcus pneumoniae, Haemophilus influenzae, members of the family Enterobacteriaceae,Pseudomonas aeruginosa, and Clostridium difficile, as well as MRSA, the novel carbapenems are worthy of further evaluation.

Download Full-text

Dissemination of the Samecfr-Carrying Plasmid among Methicillin-Resistant Staphylococcus aureus and Coagulase-Negative Staphylococcal Isolates in China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.04580-14 ◽

2015 ◽

Vol 59 (6) ◽

pp. 3669-3671 ◽

Cited By ~ 17

Author(s):

Jia Chang Cai ◽

Yan Yan Hu ◽

Hong Wei Zhou ◽

Gong-Xiang Chen ◽

Rong Zhang

Keyword(s):

Staphylococcus Aureus ◽

Methicillin Resistant Staphylococcus Aureus ◽

Mobile Element ◽

Sequence Type ◽

Coagulase Negative Staphylococci ◽

Methicillin Resistant ◽

Content Type ◽

Staphylococcal Species ◽

Complete Sequencing ◽

Staphylococcal Cassette Chromosome

ABSTRACTSixcfr-harboring methicillin-resistantStaphylococcus aureus(MRSA) isolates, which belonged to the same clone of sequence type 5 (ST5)-staphylococcal cassette chromosomemecelement II (SCCmecII)-spat311, were investigated in this study. Complete sequencing of acfr-carrying plasmid, pLRSA417, revealed an 8,487-bp fragment containing a Tn4001-like transposon,cfr,orf1, and ISEnfa4. This segment, first identified in an animal plasmid, pSS-01, was observed in several plasmids from clinical coagulase-negative staphylococci in China, suggesting that thecfrgene, which might originate from livestock, was located in the same mobile element and disseminated among different clinical staphylococcal species.

Download Full-text