Diagnostic significance of Neospora caninum DNA detected by PCR in cattle serum

2006 ◽  
Vol 142 (3-4) ◽  
pp. 207-213 ◽  
Author(s):  
Linda M. McInnes ◽  
Una M. Ryan ◽  
Ryan O’Handley ◽  
Heinz Sager ◽  
David Forshaw ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Diagnostic Significance ◽  
Cattle Serum

scholarly journals Performance of the Dot-blot test method for detecting antibodies to Sarcocystis spp. in cattle

2020 ◽  
Vol 40 (5) ◽  
pp. 385-388
Author(s):  
Maiara S.T. Ferreira ◽  
Fagner D. Fernandes ◽  
Marta E.M. Alves ◽  
Patricia Bräunig ◽  
Luis A. Sangioni ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Standard Technique ◽  
Good Alternative ◽  
Test Method ◽  
Dot Blot ◽  
Serum Samples ◽  
Sarcocystis Spp ◽  
Cattle Serum ◽  
Antibody Tests ◽  
Gold Standard Technique

ABSTRACT: Serological techniques can detect antibodies against Sarcocystis spp., Neospora caninum and Toxoplasma gondii antigens in single or mixed infections. Immunofluorescent antibody tests (IFAT) is considered the gold standard technique for Sarcocystosis diagnostic in cattle serum and a positive IFAT result reflects Sarcocystis spp. infection. Therefore, the aims of the present study were to compare IFAT and Dot-blot for sarcocystosis diagnostic in experimentally infected mice and to investigate serological cross-reactions with N. caninum and T. gondii in these methods. Mice (Mus musculus) were inoculated intraperitoneally with bradizoites of Sarcocystis spp. or tachyzoites of N. caninum or T. gondii. Serum samples were obtained and analyzed by IFAT and Dot-blot for the three protozoa. Serum from N. caninum and T. gondii experimentally infected mice were tested by IFAT and reacted only to N. caninum or T. gondii antigens, respectively. Specific antibodies against Sarcocystis spp. were present in all animals experimentally infected with this protozoan, with IFAT titers from 10 to 800. Serum samples from mice experimentally infected with Sarcocystis spp., N. caninum and T. gondii and tested by Dot-blot demonstrated no cross reaction between protozoa. A Dot-blot using Sarcocystis spp. antigen appears to be a good alternative to IFAT in the serological diagnosis of Sarcocystosis.

Diagnostic significance of computed tomography in gastric cancer

1985 ◽  
Vol 21 (5) ◽  
pp. 755
Author(s):  
E Y Kang ◽  
S H Cha ◽  
H Y Seol ◽  
K B Chung ◽  
W H Suh
Keyword(s):  
Gastric Cancer ◽  
Computed Tomography ◽  
Diagnostic Significance

SOROPREVALÊNCIA E FATORES DE RISCO PARA A INFECÇÃO POR Neospora caninum EM CÃES DE ZONA RURAL DO MUNICÍPIO DE SOUSA, PARAÍBA

2020 ◽  
Vol 4 (3) ◽  
pp. 42
Author(s):  
Samara Dos Santos Silva ◽  
Leonardo Vinícius Silva de Oliveira ◽  
Radabley Rith Almeida Oliveira ◽  
Emerson T De Alcântara Timóteo ◽  
Pollyanne Raysa Fernandes de Oliveira ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Neospora Caninum ◽  
Font Size

<p><span style="font-size: medium;"><em>Neospora caninum </em>é um protozoário cujos hospedeiros definitivos são canídeos domésticos e silvestres, e hospedeiros intermediários são os herbívoros. Objetivou-se determinar a prevalência de anticorpos IgG anti-<em>N. caninum </em>em cães de zona rural do município de Sousa, Paraíba. Foram analisadas amostras de 98 cães de zona rural, através da Reação de Imuno fluorescência Indireta no ponto de corte 1:50. A frequência de soro positividade foi de 9,18% (9/98). A titulação de anticorpos variou de 50 a 200, predominando o título 1:50. Em 66,6% (4/6) das propriedades avaliadas havia pelo menos um cão soropositivo. O contato com bovinos foi considerado fator de risco (<em>Odds ratio</em>=15,25) para a infecção, demonstrando maior risco de contato com tecidos contaminados. Conclui-se que os cães de zona rural do município de Sousa estão expostos ao <em>N. caninum</em>. </span></p>

The diagnostic and prognostic value of right ventricle systolic and diastolic function in inferior myocardial infarction patients

2018 ◽  
Vol 2 (1) ◽  
pp. 4
Author(s):  
Niniek Purwaningtyas
Keyword(s):  
Myocardial Infarction ◽  
Diastolic Function ◽  
Diagnostic Criteria ◽  
Sensitivity And Specificity ◽  
Systolic Function ◽  
Inferior Myocardial Infarction ◽  
Diagnostic Significance ◽  
St Segment Elevation ◽  
St Segment ◽  
Systolic And Diastolic Function

Background: Inferior myocardial infarction (MI) with right ventricular (RV) involvement will increase mortality and morbidity. Data of systolic and diastolic RV function in inferior ST-segment elevation MI (STEMI) are useful to predict the RV involvement.  Aims: To evaluate the prognostic and diagnostic significance of RV systolic and diastolic function compared to RVMI diagnostic criteria by electrocardiography in inferior MI patients.Methods: Consecutive patients with first, acute, inferior STEMI were prospectively assessed. The RVMI was defined as an ST-segment elevation ≥ 0.1 mV in lead V4R. Echocardiography was performed within 24 hours of the inferior STEMI symptoms. We assessed the RVMI diagnostic criteria in inferior MI patients using echocardiography.Results: Out of 31 patients (mean age 56.39 ± 9.02 years), RVMI by electrocardiography and echocardiography was found in 18 (37%). Moreover, multivariate analysis showed that two variables — RV systolic and diastolic function, were independent predictors of RVMI in inferior MI patients. Sensitivity and specificity of the RV systolic function were 94.4% and 69.2%, respectively, while RV diastolic functions were 44% and 76.9%, respectively.Conclusion: RV systolic function predict RVMI with relatively high sensitivity and specificity. RV diastolic function predicts RVMI with relatively low sensitivity but with high specificity.

Sign in / Sign up

Export Citation Format

Share Document