Determination of the lipid content of organic waste using time-domain nuclear magnetic resonance

2022 ◽  
Vol 138 ◽  
pp. 41-48
Author(s):  
S. Picard ◽  
M. Cambert ◽  
J.-M. Roger ◽  
A. Davenel ◽  
R. Girault ◽  
...  
2017 ◽  
Vol 31 (5) ◽  
pp. 5120-5125 ◽  
Author(s):  
Grazielli da Rocha ◽  
Luiz Alberto Colnago ◽  
Tiago Bueno Moraes ◽  
Giuliano Fernandes Zagonel ◽  
Graciela Ines Bolzon de Muniz ◽  
...  

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 198 ◽  
Author(s):  
João Pedroso ◽  
João Camporez ◽  
Luciana Belpiede ◽  
Rafaela Pinto ◽  
José Cipolla-Neto ◽  
...  

Devices that analyze body composition of rodents by time-domain nuclear magnetic resonance (TD-NMR) are becoming popular in research centers that study metabolism. Theoretically, TD-NMR devices can also evaluate lipid content in isolated tissues. However, the accuracy of TD-NMR to determine hepatic steatosis in the liver of small laboratory animals has not been evaluated in detail. We observed that TD-NMR was able to detect increased lipid content in the liver of rats consuming high-fat diet (HFD) for 12 weeks and in genetically obese (Lepob/ob and Leprdb/db) mice. The lipid content determined by TD-NMR showed a positive correlation with triglyceride content measured by colorimetric assays. In contrast, TD-NMR did not detect hepatic steatosis in C57BL/6 mice consuming HFD for 4 or 12 weeks, despite their obesity and increased liver triglyceride content. These findings indicate that tissue mass and the severity of hepatic steatosis affect the sensitivity of TD-NMR to detect liver lipid content.


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