scholarly journals Expanding the Efflux In Vitro Assay Toolbox: A CRISPR-Cas9 Edited MDCK Cell Line with Human BCRP and Completely Lacking Canine MDR1

2021 ◽  
Vol 110 (1) ◽  
pp. 388-396 ◽  
Author(s):  
Christine Wegler ◽  
Meryem Gazit ◽  
Karolina Issa ◽  
Sujay Subramaniam ◽  
Per Artursson ◽  
...  
Keyword(s):  
Cell Line ◽  
Mdck Cell ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Mdck Cell Line

Further characterization of the in vitro assay for inhibitors of metabolic cooperation in the Chinese hamster V79 cell line

1985 ◽  
Vol 6 (3) ◽  
pp. 361-366 ◽  
Author(s):  
C. Jone ◽  
J.E. Trosko ◽  
C.F. Aylsworth ◽  
L. Parker ◽  
C.C. Chang
Keyword(s):  
Cell Line ◽  
Chinese Hamster ◽  
In Vitro Assay ◽  
Metabolic Cooperation ◽  
Vitro Assay ◽  
V79 Cell

scholarly journals Demonstration of burst-promoting activity of recombinant human GM-CSF on circulating erythroid progenitors using an assay involving the delayed addition of erythropoietin

Blood ◽  
1985 ◽  
Vol 66 (6) ◽  
pp. 1479-1481 ◽  
Author(s):  
RE Donahue ◽  
SG Emerson ◽  
EA Wang ◽  
GG Wong ◽  
SC Clark ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Peripheral Blood ◽  
Colony Formation ◽  
In Vitro Assay ◽  
Erythroid Progenitors ◽  
Vitro Assay ◽  
Gm Csf ◽  
Semi Solid

Abstract We demonstrate through the use of an in vitro assay involving the delayed addition of erythropoietin that human recombinant GM-CSF, cloned from a mature T cell line, Mo, clearly has burst-promoting activity (BPA) on peripheral blood erythroid progenitors at picomolar concentrations. Delay for up to 72 hours of the addition of erythropoietin to semi-solid methylcellulose cultures of concentrated peripheral blood progenitors minimizes or eliminates BPA-independent erythroid colony formation with little loss of BPA-dependent erythroid colony formation. This assay will prove useful in accurately detecting sources of BPA.

scholarly journals Pigmented-MDCK (P-MDCK) Cell Line with Tunable Melanin Expression: An in Vitro Model for the Outer Blood–Retinal Barrier

2012 ◽  
Vol 9 (11) ◽  
pp. 3228-3235 ◽  
Author(s):  
Rajendra S. Kadam ◽  
Robert. I. Scheinman ◽  
Uday B. Kompella
Keyword(s):  
Cell Line ◽  
Mdck Cell ◽  
In Vitro Model ◽  
Blood Retinal Barrier ◽  
Mdck Cell Line ◽  
Vitro Model

C-Met signalling in an HGF/SF-insensitive variant MDCK cell line with constitutive motile/invasive behaviour

1996 ◽  
Vol 109 (9) ◽  
pp. 2371-2381
Author(s):  
C.P. Webb ◽  
K. Lane ◽  
A.P. Dawson ◽  
G.F. Vande Woude ◽  
R.M. Warn
Keyword(s):  
Growth Factor ◽  
Tyrosine Kinase ◽  
Cell Line ◽  
Tyrosine Phosphorylation ◽  
Mdck Cell ◽  
Mdck Cells ◽  
Cell Surface Expression ◽  
Cell Phenotype ◽  
Mdck Cell Line

The Met protein is a receptor tyrosine kinase for hepatocyte growth factor/scatter factor (HGF/SF), a multifunctional growth factor with mitogenic, motogenic and morphogenic properties. A morphologically altered variant of the MDCK cell line, MDCK-1, spontaneously exhibits a number of features associated with a partial HGF/SF-Met induced phenotype (less adhesive colonies in culture, enhanced invasion and motility, nascent tubule formation), but paradoxically does not respond to HGF/SF treatment. Although the overall cell surface expression and distribution of Met were found to be similar in parental MDCK cells and the MDCK-1 cell line, p145met autophosphorylation (+/ HGF/SF) was significantly reduced in MDCK-1 cells in vitro and in vivo when compared with parental MDCK cells. In contrast, EGF induced cell proliferation and EGF receptor autophosphorylation to similar levels in both cell lines. The basal levels of protein tyrosine phosphorylation were higher in MDCK-1 cells when compared with parental MDCK cells, including that of two prominent proteins with molecular masses of approximately 185 kDa and 220 kDa. Moreover, both p185 and p220 are present and tyrosine phosphorylated in Met immunoprecipitates from MDCK-1 cells (+/-HGF/SF), but not parental MDCK cells. In addition, Met immunocomplexes from MDCK-1 cells exhibited an approximately 3-fold increased tyrosine kinase activity in vitro when compared with MDCK cells, correlating with the higher basal levels of total phosphotyrosine. Treatment of MDCK-1 cells with the tyrosine kinase inhibitor herbimycin A reverted the cell phenotype to a more MDCK-like morphology in culture, with a concomitant reduction in the tyrosine phosphorylation predominantly of p220. Taken together these data suggest that aberrations in Met activity and associated signalling render MDCK-1 cells insensitive to HGF/SF, and may also mediate alterations in MDCK-1 cell behaviour.

A Rapid in Vitro Assay of Cellular Chemomigration in an Epithelial Carcinoma Cell Line

1995 ◽  
Vol 96 (7) ◽  
pp. 1689-1693 ◽  
Author(s):  
Edward J. Ricciardelli ◽  
John A. Persing ◽  
Jeffrey A. Romano ◽  
Raymond F. Morgan ◽  
Roy C. Ogle
Keyword(s):  
Cell Line ◽  
Carcinoma Cell ◽  
In Vitro Assay ◽  
Carcinoma Cell Line ◽  
Vitro Assay

Human intestinal Caco-2 cell line in vitro assay to evaluate the absorption of Cd, Cu, Mn and Zn from urban environmental matrices

2019 ◽  
Vol 42 (2) ◽  
pp. 601-615 ◽  
Author(s):  
Alexys Giorgia Friol Boim ◽  
Joanna Wragg ◽  
Solange Guidolin Canniatti-Brazaca ◽  
Luís Reynaldo Ferracciú Alleoni
Keyword(s):  
Cell Line ◽  
In Vitro Assay ◽  
Environmental Matrices ◽  
Vitro Assay

scholarly journals A tick cell line as a powerful tool to screen the antimicrobial susceptibility of the tick-borne pathogen Anaplasma marginale

2019 ◽  
Author(s):  
Beatriz Iglesias Alonso ◽  
Elisa Silva Ventura ◽  
Eliane Esteves ◽  
Maria F.B.M. Galletti ◽  
Bruno Dall'Agnol ◽  
...  
Keyword(s):  
Cell Line ◽  
Antimicrobial Susceptibility ◽  
Anaplasma Marginale ◽  
In Vitro Assay ◽  
Tick Cell ◽  
Vitro Assay ◽  
Tick Cell Line ◽  
Resistant Strains ◽  
Bovine Anaplasmosis

Abstract Background Anaplasma marginale is the causative agent of the severe bovine anaplasmosis. The tick Rhipicephalus microplus is one of the main vectors of A. marginale in tropical and subtropical regions of world. After the tick bite, the bacterium invades and proliferates within the bovine erythrocytes, causing anemia and impairing milk production and weight gain. In addition, infection can cause abortion and high mortality in areas of enzootic instability. The immunization with live and inactivated vaccines are employed to the control acute bovine anaplasmosis. However, they do not prevent persistent infection. Therefore, infected animals, even if immunized, are reservoirs of the bacterium and contribute to the dissemination of the disease. Antimicrobials are also largely employed for the prophylaxis of bovine anaplasmosis. However, they are often used in subtlethal doses, what can select pre-existing resistant bacteria and induce genetic or phenotypic variations. Therefore, the standardization of an in vitro assay to evaluate the susceptibility of A. marginale strains to different antimicrobials is important to allow the prescription of the more effective treatment, preventing both the selection and spread of resistant strains. Results Initially the antimicrobial susceptibility of two field isolates of A. marginale (Jaboticabal and Palmeira) infecting bovines was evaluated. The least susceptible strain (Jaboticabal) was used for the standardization of an antimicrobial assay using a culture of Ixodes scapularis-derived tick cell line, ISE6. Results showed that enrofloxacin (ENRO) at 0.25, 1 or 4 μg/mL and oxytetracycline (OTC) at 4 or 16 μg/mL are the most efficient treatments, followed by OTC at 1 μg/mL and imidocarb dipropionate (IMD) at 1 or 4 μg/mL. Conclusion In the current study, we present a new in vitro assay using a tick cell line to evaluate the susceptibility of A. marginale to antimicrobials. The maintenance of such culture is much easier than the maintenance of bovine erythrocyte culture, which depends on continuous cell replacement. This assay may be used to guide cattle farmers to the correct use of antimicrobials. The choice of the most suitable antimicrobial is essential to eliminate persistent infections, preventing the spread of resistant strains and helping in the control of bovine anaplasmosis.

Sign in / Sign up

Export Citation Format

Share Document