scholarly journals Drosophila hemolectin gene is expressed in embryonic and larval hemocytes and its knock down causes bleeding defects11Two hml-GAL4 lines (w1118; P{w + mc = GAL4-Hml} 5-6 and w1118; P{w + mc = GAL4-Hml}6-4) and one homozygote of d-hml-GAL4, UAS-gfpnlacZ, UAS-gfp[S65T]/d-hml-GAL4, UAS-gfpnlacZ, UAS-gfp[S65T] (w1118; P{w + mc = GAL4-Hml} 6-4 P{w + mc = UAS-GFP::lacZ.nls} 15.1) transgenic lines are available from the Bloomington Stock Center with the stock numbers of 6395, 6396, and 6397, respectively.

2003 ◽  
Vol 264 (2) ◽  
pp. 582-591 ◽  
Author(s):  
Akira Goto ◽  
Tatsuhiko Kadowaki ◽  
Yasuo Kitagawa
Keyword(s):  
Knock Down ◽  
Transgenic Lines

Repression of barley cathepsins, HvPap-19 and HvPap-1, differentially alters grain composition and delays germination

2021 ◽  
Author(s):  
Andrea Gomez-Sanchez ◽  
M Estrella Santamaria ◽  
Pablo Gonzalez-Melendi ◽  
Aleksandra Muszynska ◽  
Christiane Matthess ◽  
...  
Keyword(s):  
Sucrose Concentration ◽  
Starch Content ◽  
Cysteine Proteases ◽  
Artificial Microrna ◽  
Grain Composition ◽  
Knock Down ◽  
Germination Process ◽  
Lower Protein ◽  
Transgenic Lines ◽  
Livestock Feeding

Abstract During barley germination, cysteine-proteases are essential in the mobilization of storage compounds providing peptides and amino acids to sustain embryo growth until photosynthesis is completely established. Knock-down barley plants, generated by artificial microRNA, for the cathepsins B- and F-like, HvPap-19 and HvPap-1 genes, respectively, showed less cysteine protease activities and consequently lower protein degradation. The functional redundancy between proteases triggered an enzymatic compensation associated with an increase in serine-protease activities in both knock-down grains, which was not sufficient to maintain germination rates and behaviour. Concomitantly, these transgenic lines showed alterations in the accumulation of protein and carbohydrates in the grain. While the total amount of protein increased in both transgenic lines, the starch content decreased in HvPap-1 knock-down lines and the sucrose concentration was reduced in silenced HvPap-19 grains. Consequently, phenotypes of HvPap-1 and HvPap-19 artificial microRNA lines showed a delay in the grain germination process. These data expand the potential of exploring the properties of barley proteases to be selectively modified and used for brewing or livestock feeding industry.

273: Knock-Down of BCL-2 By Antisense Oligodeoxynucleotides Induces Radiosensitization and Inhibition of Angiogenesis

2007 ◽  
Vol 177 (4S) ◽  
pp. 91-92
Author(s):  
Satoshi Anai ◽  
Yoshihisa Sakai ◽  
Steve Goodison ◽  
Kathleen Shiverick ◽  
Bob Brown ◽  
...  
Keyword(s):  
Antisense Oligodeoxynucleotides ◽  
Knock Down ◽  
Inhibition Of Angiogenesis

Schutz vor Insekten

2005 ◽  
Vol 62 (11) ◽  
pp. 713-718 ◽  
Author(s):  
Rudin
Keyword(s):  
Vitamin B ◽  
Eucalyptus Citriodora ◽  
Knock Down

Erfolgreicher Schutz gegen Stiche von blutsaugenden Insekten und Zecken bedingt die konsequente Anwendung geeigneter Maßnahmen. Eine eventuell notwendige Chemoprophylaxe wird dadurch nie ersetzt. Die Umstände, unter denen der Schutz erreicht werden soll, bestimmen die Kombination der zu treffenden Maßnahmen. Von Wohnräumen kann man Insekten mit Mückengittern oder -gaze an Fenstern und Türen oder mittels Klimaanlagen fernhalten. Beim Schlafen kann man sich mit einem Moskitonetz schützen. Diese Maßnahmen können bei Bedarf durch Insektizide ergänzt oder unterstützt werden. Meistens kommen synthetische Pyrethroide entweder als «knock down»-Sprays oder elektroverdampft für die Behandlung von Räumen oder als Imprägnierungsmittel von Netzen und Gittern zum Einsatz. Wenn ein Kontakt nicht durch die Wahl von Aufenthaltsort und -zeit vermeidbar ist, werden außer Haus zum Schutz vor Stichen geeignete Kleidung sowie Repellentien eingesetzt. Kleider sollen möglichst viel Körperfläche bedecken, aus festem Gewebe, nicht eng anliegend und von heller Farbe sein. Eine zusätzliche Behandlung mit Insektiziden ist vorteilhaft. Repellentien werden direkt auf die Haut appliziert. Diethylmethylbenzamin (DEET) zeigt seit vielen Jahren eine verlässliche Wirkung. Ebenfalls verbreitete synthetische Wirkstoffe sind Bayrepel® und IR3535. Sie weisen ein noch etwas geringeres Nebenwirkungsrisiko auf, nachteilig ist jedoch die schwächere Wirkung. Von den pflanzlichen Produkten sind die mit einem Extrakt aus Eucalyptus citriodora die am besten wirksamen. Schwächere Produkte schützen Personen, die für Mücken speziell attraktiv sind, nur ungenügend. Völlig nutzlos sind auf Arm-, Halsbänder oder Kleber aufgetragene Repellentien, sowie Ultraschallgeräte, UV-Lichtfallen oder die Einnahme von Vitamin B1 oder Knoblauch.

Co-expression of AGPS and AGPL genes encoded for AGPase from cassava to enhance starch accumulation in transgenic tobacco

2016 ◽  
Vol 14 (2) ◽  
pp. 287-293
Author(s):  
Nguyễn Văn Đoài ◽  
Nguyễn Minh Hồng ◽  
Lê Thu Ngọc ◽  
Nguyễn Thị Thơm ◽  
Nguyễn Đình Trọng ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Higher Plants ◽  
Starch Content ◽  
Genetically Modified Crops ◽  
Starch Accumulation ◽  
35S Promoter ◽  
Effective Strategies ◽  
Plant Expression Vector ◽  
Transgenic Lines ◽  
Cassava Varieties

The AGPase (ADP-Glucose pyrophosphorylase) is one of the ubiquitous enzymes catalyzing the first step in starch biosynthesis. It plays an important role in regulation and adjusts the speed of the entire cycle of glycogen biosynthesis in bacteria and starch in plants. In higher plants, it is a heterotetramer and tetrameric enzyme consisting two large subunits (AGPL) and two small subunits (AGPS) and encoded by two genes. In this paper, both AGPS and AGPL genes were sucessfully isolated from cassava varieties KM140 and deposited in Genbank with accession numbers KU243124 (AGPS) and KU243122 (AGPL), these two genes were fused with P2a and inserted into plant expression vector pBI121 under the control of 35S promoter. The efficient of this construct was tested in transgenic N. tabacum. The presence and expression of AGPS and AGPL in transgenic plants were confirmed by PCR and Western hybridization. The starch content was quantified by the Anthrone method. Transgenic plant analysis indicated that that two targeted genes were expressed simultaneously in several transgenic tobacco lines under the control of CaMV 35S promoter.  The starch contents in 4 analyzed tobacco transgenic lines displays the increase 13-116%  compared to WT plants. These results indicated that the co-expression of AGPS and AGPL is one of effective strategies for enhanced starch production in plant. These results can provide a foundation for developing other genetically modified crops to increase starch accumulation capacity.

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