Molecular characterization and expression patterns of glucocorticoid receptors in the viviparous black rockfish Sebastes schlegelii

Abstract Background The black rockfish (Sebastes schlegelii) has an ovoviviparous reproductive pattern and long-term sperm storage, resulting in asynchronous gonadal development between the sexes. However, the comprehensive understanding of gonadal development in black rockfish has not yet been achieved. Here, we studied gonadal development and germ cell renewal using histology and RNA-seq. Results In this study, RNA-seq was performed on testes and ovaries to characterize key pathways and genes that are active during development and gamete maturation in black rockfish. Differentially expressed genes (DEGs) were identified and annotated in 4 comparisons (F_III vs. F_IV, F_IV vs. F_V, M_III vs. M_IV and M_IV vs. M_V). Based on analysis of DEGs enriched in the testis, 11 and 14 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were mapped to the M_III vs. M_IV group and the M_IV vs. M_V group, respectively. DEGs in ovarian development were also classified into 10 groups according to their biological functions. The expression patterns of the selected genes determined by qPCR were significantly correlated with the RNA-Seq results, supporting the reliability and accuracy of the RNA-Seq analysis. E2 levels showed down regulation from previtellogenesis to mature stage in female and T level showed down regulation from spermatogenesis to regressed stage in the male. Conclusions The categories “intercellular interaction and cytoskeleton”, “molecule amplification” and “repair in the cell cycle” were revealed to be crucial in testis development and spermatogenesis, as was the biosynthesis of a series of metabolites. Our results provide comprehensive insight into black rockfish gonadal development and provide a basis for further study of reproductive physiology and molecular biology in ovoviviparity teleosts.

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Molecular characterization, expression, and functional analysis of two thioredoxins in the black rockfish (Sebastes schlegelii)

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2012.02.002 ◽

2012 ◽

Vol 32 (5) ◽

pp. 808-815 ◽

Cited By ~ 12

Author(s):

Chan-Il Park ◽

Jee-Hyun Jung ◽

Won Joon Shim ◽

Ju-Won Kim ◽

Eun-Gyeong Kim ◽

...

Keyword(s):

Functional Analysis ◽

Molecular Characterization ◽

Black Rockfish ◽

Sebastes Schlegelii

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Molecular characterization and expression analysis of foxo3l in response to exogenous hormones in black rockfish (Sebastes schlegelii)

Gene ◽

10.1016/j.gene.2020.144777 ◽

2020 ◽

Vol 753 ◽

pp. 144777

Author(s):

Meiting Peng ◽

Minmin Sun ◽

Weihao Song ◽

Rui Li ◽

Jie Qi

Keyword(s):

Molecular Characterization ◽

Expression Analysis ◽

Black Rockfish ◽

Exogenous Hormones ◽

Sebastes Schlegelii

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Morphological and molecular characterization of Pseudocohnilembus longisetus Thompson, 1965 from farmed black rockfish Sebastes schlegelii in Korea

Veterinary Parasitology ◽

10.1016/j.vetpar.2011.02.009 ◽

2011 ◽

Vol 179 (1-3) ◽

pp. 227-233 ◽

Cited By ~ 5

Author(s):

Ilson Whang ◽

Hyun-Sil Kang ◽

Jehee Lee

Keyword(s):

Molecular Characterization ◽

Black Rockfish ◽

Sebastes Schlegelii ◽

Morphological And Molecular Characterization

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Genome-Wide Identification, Characterization and Expression Profiling of myosin Family Genes in Sebastes schlegelii

Genes ◽

10.3390/genes12060808 ◽

2021 ◽

Vol 12 (6) ◽

pp. 808

Author(s):

Chaofan Jin ◽

Mengya Wang ◽

Weihao Song ◽

Xiangfu Kong ◽

Fengyan Zhang ◽

...

Keyword(s):

Skeletal Muscle ◽

Molecular Mechanisms ◽

Expression Patterns ◽

Muscle Growth ◽

Myoblast Differentiation ◽

Growth Stages ◽

Marine Teleost ◽

Black Rockfish ◽

Skeletal Muscle Growth ◽

Sebastes Schlegelii

Myosins are important eukaryotic motor proteins that bind actin and utilize the energy of ATP hydrolysis to perform a broad range of functions such as muscle contraction, cell migration, cytokinesis, and intracellular trafficking. However, the characterization and function of myosin is poorly studied in teleost fish. In this study, we identified 60 myosin family genes in a marine teleost, black rockfish (Sebastes schlegelii), and further characterized their expression patterns. myosin showed divergent expression patterns in adult tissues, indicating they are involved in different types and compositions of muscle fibers. Among 12 subfamilies, S. schlegelii myo2 subfamily was significantly expanded, which was driven by tandem duplication events. The up-regulation of five representative genes of myo2 in the skeletal muscle during fast-growth stages of juvenile and adult S. schlegelii revealed their active role in skeletal muscle fiber synthesis. Moreover, the expression regulation of myosin during the process of myoblast differentiation in vitro suggested that they contribute to skeletal muscle growth by involvement of both myoblast proliferation and differentiation. Taken together, our work characterized myosin genes systemically and demonstrated their diverse functions in a marine teleost species. This lays foundation for the further studies of muscle growth regulation and molecular mechanisms of indeterminate skeletal muscle growth of large teleost fishes.

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Identification of Antimicrobial Peptide Genes in Black Rockfish Sebastes schlegelii and Their Responsive Mechanisms to Edwardsiella tarda Infection

Biology ◽

10.3390/biology10101015 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1015

Author(s):

Min Zhang ◽

Min Cao ◽

Yunji Xiu ◽

Qiang Fu ◽

Ning Yang ◽

...

Keyword(s):

Antimicrobial Peptide ◽

Single Molecule ◽

Molecular Mechanisms ◽

Expression Patterns ◽

Amino Acid Sequences ◽

Edwardsiella Tarda ◽

Economic Losses ◽

Dominant Type ◽

Black Rockfish ◽

Sebastes Schlegelii

The black rockfish, Sebastes schlegelii, is a typical viviparous teleost, which belongs to the family Scorpaenidae. Due to its high economic and ecological values, S. schlegelii has been widely cultured in East Asian countries. With the enlargement of cultivation scale, bacterial and viral diseases have become the main threats to the farming industry of S. schlegelii, which have resulted in significant economic losses. In this study, Illumina shotgun sequencing, single-molecule real-time (SMRT) sequencing, 10x genomics and high-throughput chromosome conformation capture (Hi-C) technologies were collectively applied to assemble the genome of S. schlegelii. Then, we identified the antimicrobial peptide genes (AMPs) in the S. schlegelii genome. In total, 214 AMPs were identified in the S. schlegelii genome, which can be divided into 33 classes according to the annotation and cataloging of the Antimicrobial Peptides Database (APD3). Among these AMPs, thrombin-derived C-terminal peptide (TCP) was the dominant type, followed by RegIIIgamma and chemokine. The amino acid sequences of the TCP, cgUbiquitin, RegIIIalpha, RegIIIgamma, chemokine shared 32.55%, 42.63%, 29.87%, 28.09%, and 32.15% similarities among the same type in S. schlegelii. Meanwhile, the expression patterns of these AMPs in nine healthy tissues and at different infection time points in intestine were investigated. The results showed that the numbers and types of AMPs that responded to Edwardsiella tarda infection gradually increased as the infection progressed. In addition, we analyzed the phylogenetic relationships of hepcidins in teleost. The identification of AMPs based on the whole genome could provide a comprehensive database of potential AMPs, and benefit for the understanding of the molecular mechanisms of immune responses to E. tarda infection in S. schlegelii. This would further offer insights into an accurate and effective design and development of AMP for aquaculture therapy in the future.

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