Lepidopteran mevalonate pathway optimization in Escherichia coli efficiently produces isoprenol analogs for next-generation biofuels

2021 ◽  
Author(s):  
Bo Pang ◽  
Jia Li ◽  
Christopher B. Eiben ◽  
Ethan Oksen ◽  
Carolina Barcelos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mevalonate Pathway ◽  
Next Generation ◽  
Pathway Optimization

scholarly journals Toward industrial production of isoprenoids in Escherichia coli : Lessons learned from CRISPR‐Cas9 based optimization of a chromosomally integrated mevalonate pathway

2018 ◽  
Vol 115 (4) ◽  
pp. 1000-1013 ◽  
Author(s):  
Jorge Alonso‐Gutierrez ◽  
Daisuke Koma ◽  
Qijun Hu ◽  
Yuchen Yang ◽  
Leanne J. G. Chan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Industrial Production ◽  
Mevalonate Pathway ◽  
Lessons Learned

scholarly journals Evaluation of the Bactericidal Activity of Plazomicin and Comparators against Multidrug-Resistant Enterobacteriaceae

2018 ◽  
Vol 62 (8) ◽  
Author(s):  
M. Thwaites ◽  
D. Hall ◽  
D. Shinabarger ◽  
A. W. Serio ◽  
K. M. Krause ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Bactericidal Activity ◽  
Minimum Bactericidal Concentration ◽  
Multidrug Resistant ◽  
Resistance Mechanisms ◽  
Next Generation ◽  
Content Type ◽  
Time Kill

ABSTRACT The next-generation aminoglycoside plazomicin, in development for infections due to multidrug-resistant (MDR) Enterobacteriaceae, was evaluated alongside comparators for bactericidal activity in minimum bactericidal concentration (MBC) and time-kill (TK) assays against MDR Enterobacteriaceae isolates with characterized aminoglycoside and β-lactam resistance mechanisms. Overall, plazomicin and colistin were the most potent, with plazomicin demonstrating an MBC50/90 of 0.5/4 μg/ml and sustained 3-log10 kill against MDR Escherichia coli, Klebsiella pneumoniae, and Enterobacter spp.

scholarly journals Bloody coli: a Gene Cocktail in Escherichia coli O104:H4

mBio ◽  
2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Fernando Baquero ◽  
Raquel Tobes
Keyword(s):  
Escherichia Coli ◽  
Next Generation Sequencing ◽  
Genetic Material ◽  
Next Generation ◽  
Content Type ◽  
Pathogenicity Genes ◽  
Sequencing Technologies ◽  
Population Sizes ◽  
Sequential Acquisition ◽  
Generation Sequencing

ABSTRACT A recent study published in mBio [Y. H. Grad et al., mBio 4(1):e00452-12, 2013] indicates that a rapid introgressive evolution has occurred in Escherichia coli O104:H4 by sequential acquisition of foreign genetic material involving pathogenicity traits. O104 genetic promiscuity cannot be readily explained by high population sizes. However, extensive interactions leading to cumulative assemblies of pathogenicity genes might be assured by small K-strategist populations exploiting particular intestinal niches. Next-generation sequencing technologies will be critical to detect particular “gene cocktails” as potentially pathogenic ensembles and to predict the risk of future outbreaks.

Production of taxadiene by engineering of mevalonate pathway in Escherichia coli and endophytic fungus Alternaria alternata TPF6

2017 ◽  
Vol 12 (4) ◽  
pp. 1600697 ◽  
Author(s):  
Guangkai Bian ◽  
Yujie Yuan ◽  
Hui Tao ◽  
Xiaofei Shi ◽  
Xiaofang Zhong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Alternaria Alternata ◽  
Endophytic Fungus ◽  
Mevalonate Pathway

Production of β-carotene and acetate in recombinant Escherichia coli with or without mevalonate pathway at different culture temperature or pH

2012 ◽  
Vol 17 (6) ◽  
pp. 1196-1204 ◽  
Author(s):  
Anh Do Quynh Nguyen ◽  
Seon-Won Kim ◽  
Sung Bae Kim ◽  
Yang-Gon Seo ◽  
In-Young Chung ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mevalonate Pathway ◽  
Recombinant Escherichia Coli ◽  
Culture Temperature ◽  
Β Carotene

Properties and inhibition of the first two enzymes of the non-mevalonate pathway of isoprenoid biosynthesis

2000 ◽  
Vol 28 (6) ◽  
pp. 792-793 ◽  
Author(s):  
C. Mueller ◽  
J. Schwender ◽  
J. Zeidler ◽  
H. K. Lichtenthaler
Keyword(s):  
Escherichia Coli ◽  
Mevalonate Pathway ◽  
Isoprenoid Biosynthesis ◽  
Mep Pathway ◽  
Ph Optimum ◽  
Antibacterial Drugs ◽  
Chloroplast Stroma

Enzymes of the 1-deoxy-D-xylulose 5-phosphate/2-C-methylerythritol 4-phosphate (DOXP/MEP) pathway are targets for new herbicides and antibacterial drugs. Until now, no inhibitors for the DOXP synthase have been known of. We show that one of the breakdown products of the herbicide clomazone affects the DOXP synthase. One inhibitor of the non-mevalonate pathway, fosmidomycin, blocks the DOXP reductoisomerase (DXR) of plants and bacteria. The I50 values of plants are, however, higher than those found for the DXR of Escherichia coli. The DXR of plants, isolated from barley seedlings, shows a pH optimum of 8.1, which is typical for enzymes active in the chloroplast stroma.

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