Understand the genomic diversity and evolution of fungal pathogen Candida glabrata by genome-wide analysis of genetic variations

Candida albicans, the major human fungal pathogen, undergoes a reversible morphological transition from blastospores (round budding cells) to filaments (elongated cells attached end-to-end). This transition, which is induced upon exposure of C. albicans cells to a number of host conditions, including serum and body temperature (37°C), is required for virulence. Using whole-genome DNA microarray analysis, we describe 61 genes that are significantly induced (≥2-fold) during the blastospore to filament transition that takes place in response to exposure to serum and 37°C. We next show that approximately half of these genes are transcriptionally repressed in the blastospore state by three transcriptional repressors, Rfg1, Nrg1, and Tup1. We conclude that the relief of this transcriptional repression plays a key role in bringing the C. albicans filamentous growth program into play, and we describe the framework of this transcriptional circuit.

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Correction to: Genome-Wide Analysis of Genetic Variations and the Detection of Rich Variants of NBS-LRR Encoding Genes in Common Wild Rice Lines

Plant Molecular Biology Reporter ◽

10.1007/s11105-018-1108-9 ◽

2018 ◽

Vol 36 (4) ◽

pp. 631-631

Author(s):

Hang Yu ◽

Muhammad Qasim Shahid ◽

Rongbai Li ◽

Wei Li ◽

Wen Liu ◽

...

Keyword(s):

Wild Rice ◽

Genetic Variations ◽

Genome Wide Analysis ◽

Common Wild Rice ◽

Genome Wide ◽

Encoding Genes

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Genome-Wide Analysis of Genetic Variations and the Detection of Rich Variants of NBS-LRR Encoding Genes in Common Wild Rice Lines

Plant Molecular Biology Reporter ◽

10.1007/s11105-018-1103-1 ◽

2018 ◽

Vol 36 (4) ◽

pp. 618-630 ◽

Cited By ~ 1

Author(s):

Hang Yu ◽

Muhammad Qasim Shahid ◽

Rongbai Li ◽

Wei Li ◽

Wen Liu ◽

...

Keyword(s):

Wild Rice ◽

Genetic Variations ◽

Genome Wide Analysis ◽

Common Wild Rice ◽

Genome Wide ◽

Encoding Genes

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Genome-wide comparison of toxigenic and non-toxigenicCorynebacterium diphtheriaeisolates identifies differences in the pan genomes between respiratory and cutaneous strains

10.1101/143800 ◽

2017 ◽

Cited By ~ 1

Author(s):

Verlaine J Timms ◽

Trang Nguyen ◽

Taryn Crighton ◽

Marion Yuen ◽

Vitali Sintchenko

Keyword(s):

Sequence Type ◽

Genomic Diversity ◽

Genome Wide Analysis ◽

Genome Wide ◽

Life Threatening ◽

Sources Of Infection ◽

Toxigenic Strains ◽

Low Prevalence ◽

Key Questions

AbstractObjectivesCorynebacterium diphtheriaeis the main etiological agent of diphtheria, a global disease causing life-threatening infections, particularly in infants and children. Vaccination with diphtheria toxoid protects against infection with potent toxin producing strains. However a growing number of apparently non-toxigenic but potentially invasiveC. diphtheriaestrains are identified in countries with low prevalence of diphtheria, raising key questions about genomic structures and population dynamics of the species.MethodsThis study examined genomic diversity among 47C. diphtheriaeisolates collected in Australia over a 10-year period using whole genome sequencing. Phylogeny was determined using SNP-based mapping and genome wide analysis.ResultsC. diphtheriaesequence type (ST) 32, a non-toxigenic ST with evidence of enhanced virulence that is also circulating in Europe, appears to be endemic in Australia. Isolates from temporospatially related patients displayed the same ST and similarity in their core genomes. The genome-wide analysis highlighted a role of pilins, adhesion factors and iron utilization in infections caused by toxigenic as well as non-toxigenic strains.ConclusionsThe genomic diversity of toxigenic and non-toxigenic strains ofC. diphtheriaein Australia suggests multiple local and overseas sources of infection and colonisation. Our findings suggest that regular genomic surveillance of co-circulating toxigenic and non-toxigenicC. diphtheriaecan deliver highly nuanced data in order to inform targeted public health actions and policy for predicting the future impact of this highly successful pathogen.

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