Out of taxonomic crypsis: A new trans-arctic cryptic species pair corroborated by phylogenetics and molecular evidence

AbstractThis paper focuses on the species diversity among the Trichostrongylidae Leiper, 1912 (Nematoda: Strongylida), and complexity of the family systematics. Polymorphism (subfamilies: Ostertagiinae, Cooperiinae and Haemonchinae), the presence of cryptic species (genus: Teladorsagia) and hybridization (genera: Cooperia, Haemonchus and Ostertagia) are presented and discussed, considering both morphological and molecular evidence. Some of these phenomena are common, nevertheless not sufficiently understood, which indicates the need for expanding the current state of knowledge thereof. Within the Trichostrongylidae, species distinction supported merely by morphological features is difficult, and requires confirmation by means of molecular methods. The parasitic nematode taxonomy is complicated mainly by the genus Teladorsagia, but complexity may also be expected among other Ostertagiinae (e.g. in the genera Ostertagia and Marshallagia). The data presented here show that the members of the Trichostrongylidae can significantly complicate unambiguous species identification. Hence, it is essential to consider the phenomena mentioned, to gather valid and comparable data on the biodiversity of this family.

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Molecular Evidence of Cryptic Species within the Liriomyza huidobrensis (Diptera: Agromyzidae)

Journal of Economic Entomology ◽

10.1603/0022-0493-93.4.1146 ◽

2000 ◽

Vol 93 (4) ◽

pp. 1146-1151 ◽

Cited By ~ 59

Author(s):

Sonja J. Scheffer

Keyword(s):

Cryptic Species ◽

Molecular Evidence ◽

Liriomyza Huidobrensis

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Molecular evidence for cryptic species among the Antarctic fish Trematomus bernacchii and Trematomus hansoni

Antarctic Science ◽

10.1017/s0954102097000485 ◽

1997 ◽

Vol 9 (4) ◽

pp. 381-385 ◽

Cited By ~ 20

Author(s):

Giacomo Bernardi ◽

Usha Goswami

Keyword(s):

Cryptic Species ◽

Dna Sequences ◽

Antarctic Fish ◽

The Other ◽

Molecular Evidence ◽

Allozyme Analysis ◽

Mcmurdo Sound ◽

Trematomus Bernacchii ◽

Colour Morphs ◽

The Antarctic

The notothenid species Trematomus bernacchii has previously been shown, by allozyme analysis, to be a complex of two cryptic species, one of which being more closely related to T. hansoni than to the other T. bernacchii cryptic species. Two T. bernacchii colour morphs, “white blotch” and “brown”, at McMurdo Sound, may correspond to these cryptic species. In this study, we present mitochondrial DNA sequences of the 12S and 16S ribosomal regions for six “white blotch” morphs, eight “brown” morphs collected in McMurdo Sound, one individual collected off the Antarctic Peninsula, and two T. hansoni individuals from McMurdo Sound. These sequences were compared with those of T. bernacchii and T. hansoni in the literature. Based on 14 phylogenetically informative sequences, no differences were found between “white blotch” and “brown” morphs. Furthermore, only one substitution separated these sequences from the previously published T. hansoni sequence, while 10 substitutions separated them from the previously published T. bernacchii sequence. Misidentified specimens, and sequence misreadings may be at the origin of these discrepancies. However, the presence of cryptic species within T. bernacchii and T. hansoni is not ruled out.

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A simple molecular identification method of the Thrips tabaci (Thysanoptera: Thripidae) cryptic species complex

Bulletin of Entomological Research ◽

10.1017/s0007485319000762 ◽

2019 ◽

Vol 110 (3) ◽

pp. 397-405 ◽

Cited By ~ 1

Author(s):

Péter Farkas ◽

Zsuzsanna György ◽

Annamária Tóth ◽

Annamária Sojnóczki ◽

József Fail

Keyword(s):

Cryptic Species ◽

Species Complex ◽

Thrips Tabaci ◽

Molecular Evidence ◽

Reproduction Rate ◽

Onion Thrips ◽

Mitochondrial Coi ◽

Wide Range ◽

Cryptic Species Complex ◽

Laboratory Colonies

AbstractThe onion thrips (Thrips tabaci Lindeman, 1889) is a key pest of a wide range of crops because of its ecological attributes such as polyphagy, high reproduction rate, ability to transmit tospoviruses and resistance to insecticides. Recent studies revealed that T. tabaci is a cryptic species complex and it has three lineages (leek-associated arrhenotokous L1-biotype, leek-associated thelytokous L2-biotype and tobacco-associated arrhenotokous T-biotype), however, the adults remain indistinguishable. T. tabaci individuals were collected from different locations of Hungary to create laboratory colonies from each biotypes. Mitochondrial COI (mtCOI) region was sequenced from morphologically identified individuals. After sequence analysis SNPs were identified and used for CAPS marker development, which were suitable for distinguishing the three T. tabaci lineages. Genetic analysis of the T. tabaci species complex based on mtCOI gene confirmed the three well-known biotypes (L1, L2, T) and a new biotype because the new molecular evidence presented in this study suggests T-biotype of T. tabaci forming two distinct (sub)clades (T1 and T2). This genetic finding indicates that the genetic variability of T. tabaci populations is still not fully mapped. We validated our developed marker on thrips individuals from our thrips colonies. The results demonstrated that the new marker effectively identifies the different T. tabaci biotypes. We believe that our reliable genotyping method will be useful in further studies focusing on T. tabaci biotypes and in pest management by scanning the composition of sympatric T. tabaci populations.

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