The use of ethylenediaminetetraacetic acid or nitrilo-triacetic acid as masking agents in the determination of palladium with dimethylglyoxime or quinaldic acid

1958 ◽  
Vol 19 ◽  
pp. 323-327 ◽  
Author(s):  
Peter F. Lott ◽  
Richard K. Vitek ◽  
K.L. Cheng
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Quinaldic Acid ◽  
Masking Agents

scholarly journals Masking Ability of Various Metal Complexing Ligands at 1.0 mM Concentrations on the Potentiometric Determination of Fluoride in Aqueous Samples

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Sakuni M. De Silva ◽  
Samitha Deraniyagala ◽  
Janitha K. Walpita ◽  
Indira Jayaweera ◽  
Saranga Diyabalanage ◽  
...  
Keyword(s):  
Experimental Data ◽  
Natural Waters ◽  
Ethylenediaminetetraacetic Acid ◽  
Aqueous Samples ◽  
Metal Chelating ◽  
Low Concentrations ◽  
Masking Agents ◽  
Potentiometric Analysis ◽  
Common Anion

Fluoride is a common anion present in natural waters. Among many analytical methods used for the quantification of fluoride in natural waters, potentiometric analysis is one of the most widely used methods because of minimum interferences from other ions commonly present in natural waters. The potentiometric analysis requires the use of ionic strength adjusting buffer abbreviated as TISAB to obtain accurate and reproducible data. In most of the reported literature, higher concentrations of strong metal chelating ligands are used as masking agents generally in the concentration range of 1.0 to 0.01 M. In the present study, effectiveness of the masking agents, phosphate, citrate, CDTA ((1,2-cyclohexylenedinitrilo)tetraacetic acid), EDTA (ethylenediaminetetraacetic acid) HE-EDTA ((hydroxyethyl)ethylenediaminetriacetic acid)), triethanolamine, and tartaric acid at 1.0 mM in TISAB solutions was investigated. The experimental data were compared with a commercially available WTW 140100 TISAB solution as the reference buffer. According to the experimental data, the reference buffer always produced the highest fluoride concentrations and the measured fluoride concentrations were in the range of 0.611 to 1.956 mg/L. Out of all the masking agents investigated, only CDTA performed marginally well and approximately a quarter of the samples produced statistically comparable data to the reference buffer. All the other masking agents produced significantly low concentrations compared to the reference buffer. The most probable reasons for the underestimation of fluoride concentrations could be shorter decomplexing time and lower masking agent concentrations.

THE DETERMINATION OF CALCIUM AND MAGNESIUM IN BLOOD USING ONE INDICATOR

1959 ◽  
Vol 37 (1) ◽  
pp. 225-229 ◽  
Author(s):  
H. M. Czajkowska Robinson ◽  
J. C. Rathbun
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Total Calcium ◽  
Eriochrome Black T ◽  
End Point ◽  
Acid Titration ◽  
Calcium And Magnesium

A rapid ethylenediaminetetraacetic acid titration for calcium and magnesium in serum requiring 0.1 ml of blood is described. Eriochrome black T is used as the sole indicator and the end point is observed photometrically. After determination of total calcium and magnesium, the calcium is removed as oxalate, the magnesium determined separately, and the calcium obtained by difference. The method is highly reproducible and is sensitive to ± 0.1 mg% of either calcium or magnesium.

scholarly journals Use of a Ruthenium(III), Iron(II), and Nickel(II) Hexacyanometallate-Modified Graphite Electrode with Immobilized Oxalate Oxidase for the Determination of Urinary Oxalate

2001 ◽  
Vol 84 (6) ◽  
pp. 1927-1933 ◽  
Author(s):  
Stjepan Milardović ◽  
Zorana Grabarić ◽  
Vlatko Rumenjak ◽  
Nenad Blau ◽  
Dražen Milošević
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Graphite Electrode ◽  
Oxalate Oxidase ◽  
Urine Samples ◽  
Daily Routine ◽  
Urinary Oxalate ◽  
Catalyzed Oxidation ◽  
Linear Concentration Range ◽  
Linear Concentration

Abstract This paper describes the performance of a biosensor with an Ru(III), Ni(II), and Fe(II) hexacyanometallate-modified graphite electrode and immobilized oxalate oxidase for the determination of urinary oxalate. The addition of ruthenium enhances the electrochemical reversibility and chemical stability of the electrocrystallized layer and improves the sensitivity of the biosensor. Hydrogen peroxide, produced by the enzyme-catalyzed oxidation of oxalate, was measured at −50 mV vs an Hg|Hg2Cl2|3M KCl electrode in a solution of pH 3.6 succinic buffer, 0.1M KCl, and 5.4mM ethylenediaminetetraacetic acid. The linear concentration range for the determination of oxalate was 0.18–280 μM. The recoveries of added oxalate (10–35 μM) from aqueous solution ranged from 99.5 to 101.7%, whereas from urine samples without oxalate (or with a concentration of oxalate below the detection limit) the recoveries of added oxalate ranged from 91.4 to 106.6%. The oxalate in 24 h urine samples, taken during their daily routine from 35 infants and children, was measured and found to range from 0.6 to 121.7 mg/L. There were no interferences from uric acid, acetylsalicylic acid, and urea in the concentration range investigated, but paracetamol and ascorbic acid did interfere. A good correlation (R2 = 0.9242) was found between values obtained for oxalate in real urine samples by 2 laboratories, with the proposed biosensor and ion chromatography, respectively.

Thin Layer Chromatographic Determination of Citrinin

1979 ◽  
Vol 62 (1) ◽  
pp. 201-202 ◽  
Author(s):  
Robert D Stubblefield
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Linear Relationship ◽  
Silica Gel ◽  
Coefficient Of Variation ◽  
Ethylenediaminetetraacetic Acid ◽  
Limit Of Detection ◽  
Chromatographic Determination ◽  
Tlc Plates

Abstract Clearly defined zones of citrinin can be obtained on thin layer chromatographic (TLC) plates and measured by fluorodensitometry. Silica gel plates were prepared as a slurry with aqueous 0.05M Na2EDTA (ethylenediaminetetraacetic acid), spread at 0.5 mm wet thickness, and activated at 105°C for 1 hr. Plates were developed in acetic acid-benzene (5+95). The limit of detection was 10 ng citrinin/zone. Densitometric analysis (365 nm excitation, 505 nm emission) revealed that a linear relationship exists for levels of 10 ng to at least 100 ng/zone wtih a coefficient of variation of ±5%.

Trace determination of gadolinium by conductivity-based approach

2020 ◽  
Vol 98 (10) ◽  
pp. 652-658
Author(s):  
R. Ananthanarayanan ◽  
M. Sivaramakrishna ◽  
B.S. Panigrahi
Keyword(s):  
Heavy Water ◽  
Ethylenediaminetetraacetic Acid ◽  
Limit Of Detection ◽  
Nuclear Industry ◽  
Complexing Agents ◽  
Complexing Agent ◽  
High Performing ◽  
New Class ◽  
Water Reactors

A conductivity-based technique is developed for the determination of Gd3+ in the heavy water moderators of pressurized heavy water reactors (PHWRs). The method involves monitoring extremely small shifts in conductivity, in the order of few nS/cm, due to the continuous addition of a suitable complexing agent to Gd3+ in aqueous medium. The resulting plot gives two distinct regions with vastly differing slopes. Two multidentate ligands, ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaaceticacid (DTPA), as complexing agents are compared. A high performing conductivity detector based on a new class of sensors called pulsating sensors that works entirely in the digital domain is deployed to monitor the conductivity shifts. Titration plots are studied in both H2O and D2O, and the observed difference between the plots in the two matrices is discussed in detail. Boron did not interfere in the analysis. The method was validated using the UV–vis spectrophotometric technique. The method is sensitive and rapid, as each analysis takes 3 min. The limit of detection in H2O and D2O are 1.27×10−7 mol/L and 5.1×10−7 mol/L, respectively. The precision in analysis lies between 1.9% and 5.3%. This method has important application in the nuclear industry for the routine analysis of gadolinium.

Concentration of dipotassium ethylenediaminetetraacetic acid but not lithium heparin affects total protein determination in equine synovial fluid

2020 ◽  
Vol 187 (8) ◽  
pp. e62-e62
Author(s):  
Pablo Jimenez Rihuete ◽  
Nicolas Villarino ◽  
Alicja Pelisiak ◽  
Luis M Rubio-Martinez
Keyword(s):  
Synovial Fluid ◽  
Cross Section ◽  
Total Protein ◽  
Gold Standard ◽  
Ethylenediaminetetraacetic Acid ◽  
Protein Determination ◽  
Collection Tube ◽  
Refractometric Determination ◽  
Biuret Method

BackgroundRefractometric determination of total protein (TP) in synovial fluid (SF) is commonly used for diagnosis and monitoring of synovial sepsis in horses. Previous studies have shown that elevated concentrations of certain anticoagulants may overestimate refractometric determination of TP concentration.ObjectivesThe aim of the study was to evaluate the effect of different concentrations of dipotassium EDTA (K2EDTA) and lithium heparin (LH) on TP determination by using a hand-held refractometer in equine synovial fluid.Study designCross-section observational study.MethodsThirty samples of synovial fluid obtained from 22 horses with different synovial conditions were collected. Synovial fluid samples were separated into different aliquots and placed in commercially available collection tubes containing K2EDTA or LH at four different concentrations (1.76, 3.52, 7.04 and 17.6 mg/ml for K2EDTA; 16, 32, 64 and 160 IU/ml for LH) . Refractometric TP determination was performed on untreated and K2EDTA and LH aliquots with a hand-held refractometer and by spectophotometric Biuret method as the gold standard.ResultsRefractometric TP determination was overestimated in SF samples containing 10 times the recommended K2EDTA concentrations. Lower concentrations of K2EDTA and LH concentrations did not affect refractometric TP determinations.Main limitationsLimited number of samples mostly obtained from large synovial structures.ConclusionTo avoid incorrect TP determination, the use of LH containing collection tubes may be an appropriate alternative when the SF volume available is not enough to fill the K2EDTA collection tube.

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