Probing sesquiterpene hydroxylase activities in a coupled assay with terpene synthases

2003 ◽  
Vol 409 (2) ◽  
pp. 385-394 ◽  
Author(s):  
Bryan T Greenhagen ◽  
Paul Griggs ◽  
Shunji Takahashi ◽  
Lyle Ralston ◽  
Joe Chappell
1991 ◽  
Vol 32 (10) ◽  
pp. 1709-1712 ◽  
Author(s):  
K Ichihara ◽  
Y Shibasaki
Keyword(s):  

DNA Research ◽  
2020 ◽  
Vol 27 (3) ◽  
Author(s):  
Nolan Bornowski ◽  
John P Hamilton ◽  
Pan Liao ◽  
Joshua C Wood ◽  
Natalia Dudareva ◽  
...  

Abstract Species within the mint family, Lamiaceae, are widely used for their culinary, cultural, and medicinal properties due to production of a wide variety of specialized metabolites, especially terpenoids. To further our understanding of genome diversity in the Lamiaceae and to provide a resource for mining biochemical pathways, we generated high-quality genome assemblies of four economically important culinary herbs, namely, sweet basil (Ocimum basilicum L.), sweet marjoram (Origanum majorana L.), oregano (Origanum vulgare L.), and rosemary (Rosmarinus officinalis L.), and characterized their terpenoid diversity through metabolite profiling and genomic analyses. A total 25 monoterpenes and 11 sesquiterpenes were identified in leaf tissue from the 4 species. Genes encoding enzymes responsible for the biosynthesis of precursors for mono- and sesqui-terpene synthases were identified in all four species. Across all 4 species, a total of 235 terpene synthases were identified, ranging from 27 in O. majorana to 137 in the tetraploid O. basilicum. This study provides valuable resources for further investigation of the genetic basis of chemodiversity in these important culinary herbs.


2018 ◽  
Vol 139 ◽  
pp. 95-100 ◽  
Author(s):  
Jyun-Liang Lin ◽  
Holly Ekas ◽  
Kelly Markham ◽  
Hal S. Alper

Cell Reports ◽  
2021 ◽  
Vol 36 (1) ◽  
pp. 109311
Author(s):  
Kouichi Yanagi ◽  
Toru Komatsu ◽  
Shusuke Ogihara ◽  
Takayoshi Okabe ◽  
Hirotatsu Kojima ◽  
...  

2018 ◽  
Vol 179 (2) ◽  
pp. 382-390 ◽  
Author(s):  
Guo Wei ◽  
Qidong Jia ◽  
Xinlu Chen ◽  
Tobias G. Köllner ◽  
Debashish Bhattacharya ◽  
...  

2002 ◽  
Vol 277 (25) ◽  
pp. 22289-22296 ◽  
Author(s):  
Anthony J. Lawrence ◽  
John G. Coote ◽  
Yasmin F. Kazi ◽  
Paul D. Lawrence ◽  
Julia MacDonald-Fyall ◽  
...  

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0253741
Author(s):  
Mihai-Silviu Tomescu ◽  
Selisha Ann Sooklal ◽  
Thuto Ntsowe ◽  
Previn Naicker ◽  
Barbara Darnhofer ◽  
...  

The corm of Hypoxis hemerocallidea, commonly known as the African potato, is used in traditional medicine to treat several medical conditions such as urinary infections, benign prostate hyperplasia, inflammatory conditions and testicular tumours. The metabolites contributing to the medicinal properties of H. hemerocallidea have been identified in several studies and, more recently, the active terpenoids of the plant were profiled. However, the biosynthetic pathways and the enzymes involved in the production of the terpene metabolites in H. hemerocallidea have not been characterised at a transcriptomic or proteomic level. In this study, total RNA extracted from the corm, leaf and flower tissues of H. hemerocallidea was sequenced on the Illumina HiSeq 2500 platform. A total of 143,549 transcripts were assembled de novo using Trinity and 107,131 transcripts were functionally annotated using the nr, GO, COG, KEGG and SWISS-PROT databases. Additionally, the proteome of the three tissues were sequenced using LC-MS/MS, revealing aspects of secondary metabolism and serving as data validation for the transcriptome. Functional annotation led to the identification of numerous terpene synthases such as nerolidol synthase, germacrene D synthase, and cycloartenol synthase amongst others. Annotations also revealed a transcript encoding the terpene synthase phytoalexin momilactone A synthase. Differential expression analysis using edgeR identified 946 transcripts differentially expressed between the three tissues and revealed that the leaf upregulates linalool synthase compared to the corm and the flower tissues. The transcriptome as well as the proteome of Hypoxis hemerocallidea presented here provide a foundation for future research.


2016 ◽  
Author(s):  
Jennifer H. Wisecaver ◽  
Alexander T. Borowsky ◽  
Vered Tzin ◽  
Georg Jander ◽  
Daniel J. Kliebenstein ◽  
...  

AbstractPlants produce a tremendous diversity of specialized metabolites (SMs) to interact with and manage their environment. A major challenge hindering efforts to tap this seemingly boundless source of pharmacopeia is the identification of SM pathways and their constituent genes. Given the well-established observation that the genes comprising a SM pathway are co-regulated in response to specific environmental conditions, we hypothesized that genes from a given SM pathway would form tight associations (modules) with each other in gene co-expression networks, facilitating their identification. To evaluate this hypothesis, we used 10 global co-expression datasets—each a meta-analysis of hundreds to thousands of expression experiments—across eight plant model organisms to identify hundreds of modules of co-expressed genes for each species. In support of our hypothesis, 15.3-52.6% of modules contained two or more known SM biosynthetic genes (e.g., cytochrome P450s, terpene synthases, and chalcone synthases), and module genes were enriched in SM functions (e.g., glucoside and flavonoid biosynthesis). Moreover, modules recovered many experimentally validated SM pathways in these plants, including all six known to form biosynthetic gene clusters (BGCs). In contrast, genes predicted based on physical proximity on a chromosome to form plant BGCs were no more co-expressed than the null distribution for neighboring genes. These results not only suggest that most predicted plant BGCs do not represent genuine SM pathways but also argue that BGCs are unlikely to be a hallmark of plant specialized metabolism. We submit that global gene co-expression is a rich, but largely untapped, data source for discovering the genetic basis and architecture of plant natural products, which can be applied even without knowledge of the genome sequence.


2021 ◽  
Author(s):  
Ankur Sarkar ◽  
Edward Kim ◽  
Taehwan Jang ◽  
Akarawin Hongdusit ◽  
Hyungjun Kim ◽  
...  

Abstract The design of small molecules that inhibit disease-relevant proteins represents a longstanding challenge of medicinal chemistry. Here, we describe an approach for encoding this challenge—the inhibition of a human drug target—into a microbial host and using it to guide the discovery and biosynthesis of targeted, biologically active natural products. This approach identified two previously unknown terpenoid inhibitors of protein tyrosine phosphatase 1B (PTP1B), an elusive therapeutic target for the treatment of diabetes and cancer. Both inhibitors appear to target an allosteric site, which confers selectivity, and can inhibit PTP1B in living cells. A screen of 24 uncharacterized terpene synthases from a pool of 4,464 genes uncovered additional hits, demonstrating a scalable discovery approach, and the incorporation of different PTPs into the microbial host yielded alternative PTP-specific detection systems. Findings illustrate the potential for using microbes to discover and build natural products that exhibit precisely defined biochemical activities yet possess unanticipated structures and/or binding sites.


RSC Advances ◽  
2021 ◽  
Vol 11 (48) ◽  
pp. 29997-30005
Author(s):  
C. Raul Gonzalez-Esquer ◽  
Bryan Ferlez ◽  
Sarathi M. Weraduwage ◽  
Henning Kirst ◽  
Alexandra T. Lantz ◽  
...  

“Insertion-engineering” approach allows for the modification of αβ terpene synthases.


Sign in / Sign up

Export Citation Format

Share Document