Conditions allowing different states of ATP- and GDP-induced permeability in mitochondria from different strains of Saccharomyces cerevisiae

During the last years there has been an increasing interest in using ethanol as a substitute for fossil fuels. The bioethanol used today is mainly produced from sugar cane and cereals, but reducing the production costs of ethanol is still crucial for a viable economic process. Cellulose from vegetable biomass will be the next cheap raw material for second generation fuel ethanol production and agricultural by-products with a low commercial value, as corn stover, corn fiber and cane bagasses would become an attractive feedstock for bioethanol production.In this study, different strains of Saccharomyces cerevisiae have been screened for the ability of bioethanol production. Yeasts were grown in a synthetic liquid medium containing sucrose in batch regime and the growth rates, ethanol and biomass productions were determined as well as their growth ability in cane molasses.The results indicate that a flocculent yeast, isolated in our lab and designated by strain F, was the most promising yeast strain among those tested for continuous ethanol production. This strain was isolated from corn hydrolysates, obtained from a Portuguese distillery facility (DVT, Torres Novas, Portugal) showing highest growth rate (0.49h-1), highest ethanol yield (0.35g/g) and high flocculation capacity.The study on ethanol production in continuous reactor process with the selected yeast strain (strain F) was made on sucrose and cane molasses at different dilution rates (0.05-0.42 h-1). A steady flocculating yeast fluidized bed reactor system was established allowing the functioning of the reactor for 1000 h. Data shows that when the dilution rate rose to 0.42h-1, the highest productivity (20g/Lh) was obtained attaining an ethanol concentration in the reactor of 47g/L for sucrose and molasses media.

Download Full-text

Biogenic amine production by Oenococcus oeni during malolactic fermentation of wines obtained using different strains of Saccharomyces cerevisiae

LWT ◽

10.1016/j.lwt.2008.08.004 ◽

2009 ◽

Vol 42 (2) ◽

pp. 525-530 ◽

Cited By ~ 15

Author(s):

Iolanda Rosi ◽

Francesca Nannelli ◽

Giovanna Giovani

Keyword(s):

Saccharomyces Cerevisiae ◽

Biogenic Amine ◽

Malolactic Fermentation ◽

Oenococcus Oeni ◽

Different Strains

Download Full-text

Redox Interactions between Saccharomyces cerevisiae and Saccharomyces uvarum in Mixed Culture under Enological Conditions

Applied and Environmental Microbiology ◽

10.1128/aem.71.1.255-260.2005 ◽

2005 ◽

Vol 71 (1) ◽

pp. 255-260 ◽

Cited By ~ 48

Author(s):

Naoufel Cheraiti ◽

StÃ¯Â¿Â½phane Guezenec ◽

Jean-Michel Salmon

Keyword(s):

Saccharomyces Cerevisiae ◽

Mixed Culture ◽

Wine Yeast ◽

Redox Status ◽

Product Formation ◽

Hybrid Strain ◽

Saccharomyces Uvarum ◽

Yeast Strains ◽

Different Strains ◽

Different Cultures

ABSTRACT Wine yeast starters that contain a mixture of different industrial yeasts with various properties may soon be introduced to the market. The mechanisms underlying the interactions between the different strains in the starter during alcoholic fermentation have never been investigated. We identified and investigated some of these interactions in a mixed culture containing two yeast strains grown under enological conditions. The inoculum contained the same amount (each) of a strain of Saccharomyces cerevisiae and a natural hybrid strain of S. cerevisiae and Saccharomyces uvarum. We identified interactions that affected biomass, by-product formation, and fermentation kinetics, and compared the redox ratios of monocultures of each strain with that of the mixed culture. The redox status of the mixed culture differed from that of the two monocultures, showing that the interactions between the yeast strains involved the diffusion of metabolite(s) within the mixed culture. Since acetaldehyde is a potential effector of fermentation, we investigated the kinetics of acetaldehyde production by the different cultures. The S. cerevisiae-S. uvarum hybrid strain produced large amounts of acetaldehyde for which the S. cerevisiae strain acted as a receiving strain in the mixed culture. Since yeast response to acetaldehyde involves the same mechanisms that participate in the response to other forms of stress, the acetaldehyde exchange between the two strains could play an important role in inhibiting some yeast strains and allowing the growth of others. Such interactions could be of particular importance in understanding the ecology of the colonization of complex fermentation media by S. cerevisiae.

Download Full-text

Enzyme-constrained models predict the dynamics of Saccharomyces cerevisiae growth in continuous, batch and fed-batch bioreactors

10.1101/2021.07.22.453334 ◽

2021 ◽

Author(s):

Sara Moreno-Paz ◽

Joep Schmitz ◽

Vitor A.P. Martins dos Santos ◽

Maria Suarez-Diez

Keyword(s):

Saccharomyces Cerevisiae ◽

Model Organism ◽

Growth Dynamics ◽

Operational Conditions ◽

Strain Design ◽

Balance Analysis ◽

Different Strains ◽

Batch Bioreactors ◽

Constrained Models ◽

Genome Scale

Genome-scale, constraint-based models (GEM) and their derivatives are commonly used to model and gain insights into microbial metabolism. Often, however, their accuracy and predictive power are limited and enable only approximate designs. To improve their usefulness for strain and bio-process design, we studied here their capacity to accurately predict metabolic changes in response to operational conditions in a bioreactor, as well as intracellular, active reactions. We used flux balance analysis (FBA) and dynamic FBA (dFBA) to predict growth dynamics of the model organism Saccharomyces cerevisiae under different industrially relevant conditions. We compared simulations with the latest developed GEM for this organism (Yeast8) and its enzyme-constrained version (ecYeast8) herein described with experimental data and found that ecYeast8 outperforms Yeast8 in all the simulations. EcYeast8 was able to predict well-known traits of yeast metabolism including the onset of the Crabtree effect, the order of substrate consumption during mixed carbon cultivation and production of a target metabolite. We showed how the combination of ecGEM and dFBA links reactor operation and genetic modifications to flux predictions, enabling the prediction of yields and productivities of different strains and (dynamic) production processes. Additionally, we present flux sampling as a tool to analyze flux predictions of ecGEM, of major importance for strain design applications. We showed that constraining protein availability substantially improves accuracy of the description of the metabolic state of the cell under dynamic conditions. This therefore enables more realistic and faithful designs of industrially relevant cell-based processes and, thus, the usefulness of such models

Download Full-text

Relationship between H2S-producing strains of wine yeast and different fermentation conditions

Canadian Journal of Microbiology ◽

10.1139/w99-010 ◽

1999 ◽

Vol 45 (4) ◽

pp. 343-346 ◽

Cited By ~ 5

Author(s):

C Tamayo ◽

J Ubeda ◽

A Briones

Keyword(s):

Saccharomyces Cerevisiae ◽

Stainless Steel ◽

Hydrogen Sulphide ◽

Yeast Strain ◽

Alcoholic Fermentation ◽

Wine Yeast ◽

Fermentation Conditions ◽

Factors Affecting ◽

Different Strains ◽

The Relationship

Hydrogen sulphide formation is a problem in winemaking. One of the factors affecting formation of this unwanted metabolite is the yeast strain responsible for the process. In this experiment wines were made on a laboratory scale with different strains of H2S-producing Saccharomyces cerevisiae. The relationship between H2S production and various fermentation conditions was examined (SO2, methionine, (NH4)2SO4, (NH4)3PO4, steel, and steel-lees). The results show that in fermentations in the presence of stainless steel and lees, H2S formation is high but declines when (NH4)3PO4is added to the must.Key words: H2S formation, wine-yeast, steel-lees, wine-making, alcoholic fermentation.

Download Full-text

Rearrangements of highly polymorphic regions near telomeres of Saccharomyces cerevisiae

Molecular and Cellular Biology ◽

10.1128/mcb.4.11.2509-2517.1984 ◽

1984 ◽

Vol 4 (11) ◽

pp. 2509-2517

Author(s):

H Horowitz ◽

P Thorburn ◽

J E Haber

Keyword(s):

Saccharomyces Cerevisiae ◽

Homologous Sequence ◽

Tetrad Analysis ◽

Homologous Chromosomes ◽

Restriction Fragments ◽

Yeast Strains ◽

Number Of Genes ◽

Restriction Endonuclease Fragment ◽

Different Strains ◽

Gene Conversions

We have examined the mitotic and meiotic properties of telomeric regions in various laboratory strains of yeast. Using a sequence (Y probe) derived from a cloned yeast telomere (J. Szostak and E. Blackburn, Cell 29:245-255, 1982), we found that various strains of Saccharomyces cerevisiae show extensive polymorphisms of restriction endonuclease fragment length. Some of the variation in the lengths of telomeric fragments appears to be under the control of a small number of genes. When DNA from various strains was digested with endonuclease KpnI, nearly all of the fragments homologous to the Y probe were found to be of different size. The pattern of fragments in different strains was extremely variable, with a greater degree of polymorphism than that observed for fragments containing the mobile TY1 element. Tetrad analysis of haploid meiotic segregants from diploids heterozygous for many different Y-homologous KpnI fragments revealed that most of them exhibited Mendelian (2:0) segregation. However, only a small proportion of these fragments displayed the obligate 2:2 parental segregation expected of simple allelic variants at the same chromosome end. From the segregations of these fragments, we concluded that some yeast telomeres lack a Y-homologous sequence and that the chromosome arms containing a Y-homologous sequence are different among various yeast strains. Regions near yeast telomeres frequently undergo rearrangement. Among eight tetrads from three different diploids, we have found three novel Y-homologous restriction fragments that appear to have arisen during meiosis. In all three cases, the appearance of a new fragment was accompanied by the loss of another band. In one of these cases, the rearrangement leading to a novel fragment arose in an isogenic diploid, in which both homologous chromosomes should have been identical. Among these same tetrads we also found examples of apparent mitotic gene conversions and mitotic recombination involving telemetric regions.

Download Full-text

Many yeast chromosomes lack the telomere-specific Y' sequence

Molecular and Cellular Biology ◽

10.1128/mcb.9.12.5754-5757.1989 ◽

1989 ◽

Vol 9 (12) ◽

pp. 5754-5757

Author(s):

D Jäger ◽

P Philippsen

Keyword(s):

Saccharomyces Cerevisiae ◽

Dna Hybridization ◽

Gel Electrophoresis ◽

Pulsed Field Gel Electrophoresis ◽

Pulsed Field ◽

Different Strains

Chromosomal DNAs of 26 different strains representing Saccharomyces species were analyzed by pulsed-field gel electrophoresis and subsequent hybridization to Y' telomere DNA. Hybridization to Y' was found exclusively in Saccharomyces cerevisiae strains, and among these strains, Y' sequences were found to be lacking in small, middle-sized, and large chromosomes.

Download Full-text

Identification des souches de levures isolées de vins par l'analyse de leur ADN mitochondrial

OENO One ◽

10.20870/oeno-one.1987.21.4.1286 ◽

1987 ◽

Vol 21 (4) ◽

pp. 267 ◽

Cited By ~ 1

Author(s):

Denis Dubourdieu ◽

Aline Sokol ◽

Joseph Zucca ◽

Patrick Thalouarn ◽

Agnès Dattee ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Mitochondrial Dna ◽

Restriction Patterns ◽

Adn Mitochondrial ◽

Practical Applications ◽

Mise En Œuvre ◽

Dna Restriction ◽

Wild Yeasts ◽

Different Strains

L'analyse des profils de restriction de l'ADN mitochondrial des levures permet une caractérisation fine des souches de Saccharomyces cerevisiae. Cette analyse a été appliquée à deux souches de levures sèches actives et à une vingtaine de souches indigènes, isolées de différents moûts lors de la fermentation spontanée.Les profils de restriction de l'ADN mt des souches étudiées présentent une grande diversité.La méthode mise en oeuvre est décrite de façon détaillée et les applications pratiques discutées.+++The analysis of restriction patterns from yeast's mitochondrial DNA leads to a fine characterization of different strains of Saccharomyces cerevisiae. This analysis has been applied to two commercial strains and to twenty wild yeasts, isolated from different musts in case of natural fermentations.The DNA restriction patterns of the strains studied present a wide diversity.The method we used is minutely described and the practical applications are discussed.

Download Full-text

Many yeast chromosomes lack the telomere-specific Y' sequence.

Molecular and Cellular Biology ◽

10.1128/mcb.9.12.5754 ◽

1989 ◽

Vol 9 (12) ◽

pp. 5754-5757 ◽

Cited By ~ 26

Author(s):

D Jäger ◽

P Philippsen

Keyword(s):

Saccharomyces Cerevisiae ◽

Dna Hybridization ◽

Gel Electrophoresis ◽

Pulsed Field Gel Electrophoresis ◽

Pulsed Field ◽

Different Strains

Chromosomal DNAs of 26 different strains representing Saccharomyces species were analyzed by pulsed-field gel electrophoresis and subsequent hybridization to Y' telomere DNA. Hybridization to Y' was found exclusively in Saccharomyces cerevisiae strains, and among these strains, Y' sequences were found to be lacking in small, middle-sized, and large chromosomes.

Download Full-text

Saccharomyces cerevisiae Strain Diversity Associated with Spontaneous Fermentations in Organic Wineries from Galicia (NW Spain)

Fermentation ◽

10.3390/fermentation6030089 ◽

2020 ◽

Vol 6 (3) ◽

pp. 89

Author(s):

David Castrillo ◽

Noemi Neira ◽

Pilar Blanco

Keyword(s):

Saccharomyces Cerevisiae ◽

Statistical Tests ◽

Wide Distribution ◽

Nw Spain ◽

Wine Quality ◽

Spontaneous Fermentation ◽

Strain Diversity ◽

Spontaneous Fermentations ◽

Different Strains ◽

Yeast Isolation

Yeast play an essential role in wine quality. The dynamics of yeast strains during fermentation determine the final chemical and sensory characteristics of wines. This study aims to evaluate the Saccharomyces cerevisiae strains diversity in organic wineries from Galicia (NW Spain). Samples from spontaneous fermentations were taken in five wineries over three consecutive years (2013 to 2015). The samples were transported to the laboratory and processed following standard methodology for yeast isolation. S. cerevisiae strains were differentiated by mDNA-RFLPs. A total of 66 different strains were identified. Some of them presented a wide distribution and appeared in several wineries. However, other strains were typical from a specific winery. Similarity analysis using two different statistical tests showed significant differences in strain diversity among wineries. The results also revealed high biodiversity indexes; however, only some strains showed an important incidence in their distribution and frequency. Our findings confirmed that spontaneous fermentation favored the existence of a high S. cerevisiae strain diversity in organic wineries from Galicia. The presence of different yeasts during fermentation, specially winery-specific strains, contribute to increased wine complexity and differentiation.

Download Full-text