U1 small nuclear RNA-like sequences in human high molecular weight RNA

1984 ◽  
Vol 122 (2) ◽  
pp. 838-844 ◽  
Author(s):  
J.M. Capasso ◽  
M.A. Docherty ◽  
A. Ray ◽  
E.D. Kaplan ◽  
G.L. Eliceiri
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Small Nuclear Rna ◽  
Nuclear Rna

Metabolism of high molecular weight, polydisperse, rapidly labeled nuclear RNA in rat liver

1968 ◽  
Vol 46 (12) ◽  
pp. 1497-1505 ◽  
Author(s):  
Maurice Brossard ◽  
Louis Nicole
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Rat Liver ◽  
Ribosomal Rna ◽  
Base Composition ◽  
Orotic Acid ◽  
Chromosomal Origin ◽  
Base Analysis ◽  
Nuclear Rna ◽  
Kinetics Of

Studies of the metabolism of rat liver RNA showed the existence of two species of rapidly labeled nuclear RNA: a 45 S preribosomal type of nucleolar origin, and a 6–50 S polydisperse RNA of chromosomal origin. The kinetics of labeling with orotic acid-14C and the nature of the latter RNA have been investigated. The following findings are reported, (1) This RNA is composed of at least four main classes of RNA having sedimentation coefficients of approximately 45, 35, 24, and 18 S. (2) Except for the 18 S class which seems to be an end product, the three other classes have a rapid turnover and do not appear to leave the nucleus. (3) Base analysis after 32P incorporation indicates that these four classes of RNA have a similar base composition with a G+C/A + U ratio in the range of 0.98–1.07, which resembles DNA more closely than ribosomal RNA. (4) The 6–50 S polydisperse RNA has a different metabolism than that of the 45 S preribosomal type and there is no precursor-to-product relationship between these two species of RNA.

scholarly journals Characterization of uterine heterogeneous nuclear ribonucleic acid and the effect of oestradiol-17β on its synthesis

1978 ◽  
Vol 172 (3) ◽  
pp. 587-593 ◽  
Author(s):  
S Aziz ◽  
J T Knowler
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Early Response ◽  
Oestrogen Treatment ◽  
Size Profile ◽  
Immature Rats ◽  
Nuclear Rna ◽  
Kinetics Of ◽  
Molecular Weight Fractions

An early response to the administration of oestradiol-17 beta to immature rats is a dramatic stimulation in the synthesis of uterine hnRNA (heterogenous nuclear RNA). High-molecular-weight fractions of the hnRNA were purified and subfractionated on poly(U)-Sepharose into fractions that differed in their poly(A) content and their size profile on polyacrylamide gels. Oestrogen treatment of the rats stimulated the synthesis of all three fractions of high-molecular-weight hnRNA, but the kinetics of synthesis, degree of stimulation and size distribution of the newly synthesize RNA differed in each fraction.

scholarly journals Small nuclear ribonucleoproteins of Drosophila: identification of U1 RNA-associated proteins and their behavior during heat shock.

1982 ◽  
Vol 2 (8) ◽  
pp. 914-920 ◽  
Author(s):  
E D Wieben ◽  
T Pederson
Keyword(s):  
Molecular Weight ◽  
Heat Shock ◽  
Nuclear Proteins ◽  
Small Nuclear Rna ◽  
Shock Response ◽  
Nuclear Rna ◽  
Associated Proteins ◽  
Protein Complement ◽  
Mammalian Counterpart ◽  
Small Nuclear Ribonucleoproteins

In Drosophila, two nuclear proteins of approximately 26,000 and 14,000 molecular weight are recognized by a human autoimmune antibody for mammalian ribonucleoprotein (RNP) particles that contain U1 small nuclear RNA. The antibody-selected Drosophila RNP contains, in addition to these two proteins, a single RNA species that has been identified as U1 by hybridization with a cloned Drosophila U1 DNA probe. Small nuclear RNP isolated from human cells under the same conditions as used for Drosophila and selected by the anti-U1 RNP-specific antibody contains eight proteins, two of which are similar in molecular weight to the two Drosophila U1 RNP proteins. Thus, even though the nucleotide sequences of Drosophila and human U1 RNA are about 72% homologous, and the corresponding RNPs are both recognized by the same human autoantibody, Drosophila U1 RNP appears to have a simpler protein complement than its mammalian counterpart. The two Drosophila U1 RNA-associated proteins are synthesized at normal or slightly increased rates during the heat shock response and are incorporated into antibody-recognizable RNP complexes. This raises the possibility that U1 RNP is an indispensable nuclear element for cell survival during heat shock.

scholarly journals Differential subnuclear distribution of polyadenylate-rich ribonuclei acid during induction of egg-yolk protein synthesis in male Xenopus liver by oestradiol-17β

1975 ◽  
Vol 150 (3) ◽  
pp. 345-355 ◽  
Author(s):  
J R Tata ◽  
B Baker
Keyword(s):  
Molecular Weight ◽  
Protein Synthesis ◽  
High Molecular Weight ◽  
Sodium Dodecyl ◽  
Fold Increase ◽  
Egg Yolk ◽  
Yolk Protein ◽  
Stable Form ◽  
Nuclear Rna ◽  
Vitellogenin Mrna

A 4-8-fold increase in the rate of hepatic nuclear RNA synthesis occurred within 11 h after a single injection of oestradiol-17 β to male Xenopus to induce egg-yolk protein synthesis. 2. By using a gentle procedure for fractionating nuclei into their major structurally different components [J. R. Tata& B. Baker (1974) Exp. Cell Res. 83. 111-124], it was found that the hormone-induced increase in the total amount of newly made RNA was associated with a 2-10-fold increase in the poly(A) content of nuclear RNA. 3. When the poly (A) content of nuclear RNA was determined by hybridization to poly[3H](U) or specific binding to oligo(dT)-cellulose, most of the increase (10-fold) in poly (A) content of newly synthesized RNA was associated with the euchromatin fractions, whereas the increase was less marked in the other subnuclear fractions. 4. Resolution of nuclear RNA into poly (A)-poor and poly(A)-rich RNA species by chromatography on oligo(dT)-cellulose, followed by polyacrylamide-gel electrophoresis with sodium dodecyl sulphate or in the pressence of 99% formamide, revealed that the hormone caused a preferential enhancement of high-molecular-weight (25S-60S) poly (A)-rich HnRNA (heterogeneous nuclear RNA,) much of which was associated with euchromatin and not with the nuclear sap. 5. Induction of vitellogenin in male frogs was in particular characterized by the appearance of a high-molecular-weight polyadenylated component exhibiting a peak at 35-36S, i.e. a molecular weight of approx. 2.05×10(6)+∕-0.15×10(6). Although there is no evidence as yet that such a polyadenylated high-molecular-weight nuclear RNA species contains sequences corresponding to vitellogenin mRNA, it is possible that a high proportion of the most stable form of the putative nuclear precursor to vitellogenin mRNA induced by oestrogen in male Xenopus liver may be only marginally bigger than the cytoplasmic mRNA, and may at any one time be predominantly associated with the euchromatin fraction.

scholarly journals The detection of messenger ribonucleic acid sequences in heterogeneous nuclear ribonucleic acid fractions of the oestrogen-stimulated rat uterus

1980 ◽  
Vol 187 (1) ◽  
pp. 265-267 ◽  
Author(s):  
S Aziz ◽  
J T Knowler
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ribonucleic Acid ◽  
Mrna Sequence ◽  
Rat Uterus ◽  
Complementary Dna ◽  
Messenger Ribonucleic Acid ◽  
Complementary Sequences ◽  
Nuclear Rna

cDNA (complementary DNA) complementary to the abundant sequences of mRNA isolated from oestrogen-stimulated uterus was hybridized to polyadenylated and non-polyadenylated uterine hnRNA (heterogeneous nuclear RNA). High-molecular-weight polyadenylated hnRNA, isolated under denaturing conditions, was able to saturate the cDNA, but complementary sequences were low abundance when compared with homologous mRNA. The mRNA sequence content of the polyadenylated hnRNA increased considerably during oestrogen-induced growth of the uterus. Non-polyadenylated hnRNA also contained sequences complementary to the cDNA.

Polyoma virus high molecular weight nuclear rna codes for capsid protein VP2 in vitro

Virology ◽  
1978 ◽  
Vol 89 (2) ◽  
pp. 461-474 ◽  
Author(s):  
Robert I. Kamen ◽  
Tricia Wheeler ◽  
Alan E. Smith
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Capsid Protein ◽  
Polyoma Virus ◽  
Nuclear Rna

Small nuclear ribonucleoproteins of Drosophila: identification of U1 RNA-associated proteins and their behavior during heat shock

1982 ◽  
Vol 2 (8) ◽  
pp. 914-920
Author(s):  
E D Wieben ◽  
T Pederson
Keyword(s):  
Molecular Weight ◽  
Heat Shock ◽  
Nuclear Proteins ◽  
Small Nuclear Rna ◽  
Shock Response ◽  
Nuclear Rna ◽  
Associated Proteins ◽  
Protein Complement ◽  
Mammalian Counterpart ◽  
Small Nuclear Ribonucleoproteins

In Drosophila, two nuclear proteins of approximately 26,000 and 14,000 molecular weight are recognized by a human autoimmune antibody for mammalian ribonucleoprotein (RNP) particles that contain U1 small nuclear RNA. The antibody-selected Drosophila RNP contains, in addition to these two proteins, a single RNA species that has been identified as U1 by hybridization with a cloned Drosophila U1 DNA probe. Small nuclear RNP isolated from human cells under the same conditions as used for Drosophila and selected by the anti-U1 RNP-specific antibody contains eight proteins, two of which are similar in molecular weight to the two Drosophila U1 RNP proteins. Thus, even though the nucleotide sequences of Drosophila and human U1 RNA are about 72% homologous, and the corresponding RNPs are both recognized by the same human autoantibody, Drosophila U1 RNP appears to have a simpler protein complement than its mammalian counterpart. The two Drosophila U1 RNA-associated proteins are synthesized at normal or slightly increased rates during the heat shock response and are incorporated into antibody-recognizable RNP complexes. This raises the possibility that U1 RNP is an indispensable nuclear element for cell survival during heat shock.

ISOLATION AND COMPOSITION OF NUCLEI AND NUCLEOLI

1972 ◽  
Vol 71 (2_Suppla) ◽  
pp. S35-S66 ◽  
Author(s):  
Harris Busch ◽  
Yong C. Choi ◽  
Ierachmiel Daskal ◽  
Akira Inagaki ◽  
Mark O. J. Olson ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Low Molecular Weight ◽  
New Information ◽  
Nuclear Rna ◽  
Acidic Proteins ◽  
Nuclear Structures ◽  
Nucleolar Components

ABSTRACT The past decade has brought about the evolution of increasingly precise methods for isolation of nuclei and nucleoli and their components including histones, acidic proteins, high molecular weight RNA, low molecular weight RNA and ribonucleoproteins. The available methods and improving methods permit the isolation of individual unique molecular species in high states of purity. Elegant studies have been made on the amino acid sequences of some of the histones and on the nucleotide sequences of some of the species of low molecular weight RNAs uniquely found in the nucleus. In addition, nucleotide sequences are being defined for fragments of high molecular weight nuclear RNA. In studies on the effects of hormones on cells, the roles of repressor and derepressor acidic nuclear proteins are now being defined. The next decade should bring extensive new information regarding nuclear structures and functions and nucleolar components and their structures and functions.

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