Nonproteolytic activation of the thrombin receptor promotes human umbilical vein endothelial cell growth but not intracellular Ca2+, prostacyclin, or permeability

1997 ◽  
Vol 53 (4) ◽  
pp. 487-491 ◽  
Author(s):  
Paul Schaeffer ◽  
Elisabeth Riera ◽  
Evelyne Dupuy ◽  
Jean-Marc Herbert
Keyword(s):  
Endothelial Cell ◽  
Cell Growth ◽  
Umbilical Vein ◽  
Thrombin Receptor ◽  
Human Umbilical Vein ◽  
Endothelial Cell Growth

scholarly journals Effects of Collagens on Human Umbilical Vein Endothelial Cell Growth

1987 ◽  
Vol 15 (3) ◽  
pp. 803-804
Author(s):  
Kazutomi KANEMARU ◽  
Toshiro OOYAMA ◽  
Hiromi ODA ◽  
Yasuko KOSHIHARA
Keyword(s):  
Endothelial Cell ◽  
Cell Growth ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Endothelial Cell Growth

Adenosine stimulates proliferation of human endothelial cells in culture

1993 ◽  
Vol 265 (1) ◽  
pp. H131-H138 ◽  
Author(s):  
M. F. Ethier ◽  
V. Chander ◽  
J. G. Dobson
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Growth ◽  
Cell Cultures ◽  
Umbilical Vein ◽  
Fibroblast Cell ◽  
Human Endothelial Cells ◽  
Cell Counts ◽  
Endothelial Cell Cultures ◽  
Endothelial Cell Growth

The effect of adenosine on proliferation of human endothelial cells was investigated by adding adenosine to the medium of cultures derived from human umbilical veins. Cell counts on cultures grown in 10 microM adenosine for 4–7 days were 41–53% greater than counts from control cultures. In contrast, 10 microM adenosine had no effect on growth of a human fibroblast cell strain (IMR-90). Neither inosine nor 2',5'-dideoxyadenosine influenced endothelial cell growth at concentrations of 0.1 or 10 microM. Addition of adenosine deaminase abolished the proliferative effect of added adenosine and inhibited proliferation by 16% in control cultures, suggesting that endogenous adenosine may enhance proliferation in culture. The adenosine receptor antagonist, 8-phenyltheophylline, at 0.1 and 1.0 microM blocked the enhanced proliferation caused by 10 microM adenosine. Addition of 10 microM adenosine enhanced DNA synthesis in endothelial cell cultures as indicated by an increased incorporation of [3H]thymidine into acid-insoluble cell material. The results indicate that addition of physiological concentrations of adenosine to human umbilical vein endothelial cell cultures stimulates proliferation, possibly via a surface receptor, and suggest that adenosine may be a factor for human endothelial cell growth and possibly angiogenesis.

scholarly journals Serial propagation of human endothelial cells in vitro.

1981 ◽  
Vol 91 (2) ◽  
pp. 420-426 ◽  
Author(s):  
T Maciag ◽  
G A Hoover ◽  
M B Stemerman ◽  
R Weinstein
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Cell Growth ◽  
Umbilical Vein ◽  
Maximum Growth ◽  
High Serum ◽  
Endothelial Cell Growth ◽  
Cell Densities ◽  
Population Doublings

Human umbilical vein (HUV) endothelial cells were grown for 15 to 21 passages at a split ratio of 1:5 (at least 27 population doublings) on a human fibronectin (HFN) matrix in Medium 199 supplemented with fetal bovine serum (FBS) and endothelial-cell growth factor (ECGF). This system also permitted the growth of HUV endothelial cells at cell densities as low as 1.25 cells/cm2. In addition to delaying the premature senescence of HUV endothelial cells, ECGF also reduced the serum requirement for low-density HUV endothelial-cell growth; 2.5% serum and ECGF yields half-maximum growth as compared to high serum controls. Significant HUV endothelial-cell growth was also observed in medium supplemented with either ovine hypophysectomized (HYPOX) serum, plasma-derived serum (PDS), or HYPOX-PDS in the presence of ECGF, suggesting that neither the pituitary nor the platelet contributes to HUV endothelial-cell growth.

Thrombomodulin Modulates the Mitogenic Response to Thrombin of Human Umbilical Vein Endothelial Cells

1998 ◽  
Vol 79 (04) ◽  
pp. 848-852 ◽  
Author(s):  
Monique Lafay ◽  
Reyes Laguna ◽  
Bernard Le Bonniec ◽  
Dominique Lasne ◽  
Martine Aiach ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Endothelial Cell ◽  
Umbilical Vein ◽  
Thrombin Receptor ◽  
Mitogenic Response ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells ◽  
Huvec Proliferation

SummaryThrombin interacts with its receptor and thrombomodulin on endothelial cells. We evaluated the respective roles of these two proteins on human umbilical vein endothelial cell (HUVEC) growth by comparing thrombin, S195A (a mutant thrombin in which the serine of the charge stabilizing system had been replaced by alanine), and the receptor activating peptide (TRAP). Thrombin and TRAP induced DNA synthesis (half maximal cell proliferation with 5 nM and 25 μM, respectively), whereas S195A thrombin was inactive, inferring that growth is mediated through the thrombin receptor. Surprisingly, cells stimulated by TRAP exhibited a maximal proliferation twice greater than that obtained with thrombin. Combination of thrombin and TRAP resulted in a mitogenic response higher than by thrombin alone, but lower than by TRAP alone. The role of thrombomodulin was evaluated by adding an anti-thrombomodulin antibody, which prevents formation of the thrombin-thrombomodulin complex. Antibody did not interfere with cell proliferation induced by TRAP, but enhanced that induced by thrombin. We conclude that formation of the thrombin-thrombomodulin complex restrains HUVEC proliferation mediated through the thrombin receptor.

Proliferation of endothelial cells (HUVEC) on specific-modified collagen sponges loaded with different growth factors

2021 ◽  
pp. 039139882110431
Author(s):  
Andreas Groger ◽  
Ioannis-Fivos Megas ◽  
Ernst Magnus Noah ◽  
Norbert Pallua ◽  
Gerrit Grieb
Keyword(s):  
Endothelial Cells ◽  
Growth Factors ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
Pore Size ◽  
Structural Integrity ◽  
Umbilical Vein ◽  
Additional Increase ◽  
Endothelial Cell Growth

In general, matrices for tissue engineering must maintain structural integrity during the process of tissue formation and promote vascularization of developing tissue. Therefore, collagen sponges, manufactured by an approach that offers the potential of unidirectional pore size, were seeded with human umbilical vein endothelial cells (HUVEC) to demonstrate a positive effect on cell proliferation. In addition, vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) have been used to promote proliferation of HUVEC on optimized collagen sponges. Growth and viability of the cells were evaluated. Potential unidirectional pore structure demonstrated an improvement of both, endothelial cell growth and viability. Supplementation of growth factors showed an additional increase of endothelial cell growth on collagen sponges, which confirmed the high potential of combining this biomaterial with growth factors. The results suggest that a collagen sponge with a potential specific pore size could be a suitable scaffold for endothelial cells and might be a promising implantable biomaterial with enhanced angiogenic capabilities for future clinical applications.

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