A Simple Method for Following Rigor Mortis Development in Beef and Poultry Meat

The aim of this review was to summarize the histological, physical, chemical and microbial aspects and ways to stop the fluctuation in meat quality in addition to enhance its sensory quality using electrical stimulation. The low-voltage electrical stimulation and high-voltage electrical stimulation were employed. Electrical stimulation improves quality of poultry meat by reducing shear, increasing Sarcomere length, and reducing the diameter of muscle fibers. Low-voltage electrical stimulation affects voltages (120 volts directly in the nervous system, while high voltages were more than enough) to remove the polarization of the cover and produce a dense physio-chemical response in the muscles that had a direct effect. Electrical stimulation and injection with salts of sodium chloride and calcium chloride in the carcasses of female goats improved the tenderness of the meat. It was found that electrical stimulation accelerated the development of rigor mortis. Although electrical stimulation reduces breast meat shear, there was limited information about the effects of electrical stimulation on the other quality attributes of poultry meat and the results vary with quality attribute and differ from experimental conditions High voltage (higher than 120 volts) electrical stimulation improved meat quality and decreased the pH. Low voltages (below 120 volts) are used to ensure the safety of workers and give the desired results when used in commercial applications.

Download Full-text

Polymerase Chain Reaction–Restriction Fragment Length Polymorphism of Mitochondrial 12S rRNA Gene: A Simple Method for Identification of Poultry Meat Species

Veterinary Research Communications ◽

10.1007/s11259-006-3390-5 ◽

2007 ◽

Vol 31 (4) ◽

pp. 447-455 ◽

Cited By ~ 34

Author(s):

P. S. Girish ◽

A. S. R. Anjaneyulu ◽

K. N. Viswas ◽

F. H. Santhosh ◽

K. N. Bhilegaonkar ◽

...

Keyword(s):

Fragment Length Polymorphism ◽

Poultry Meat ◽

12S Rrna ◽

Rrna Gene ◽

Chain Reaction ◽

Simple Method ◽

12S Rrna Gene ◽

Meat Species ◽

Mitochondrial 12S Rrna ◽

Polymerase Chain

Download Full-text

A simple way for producing holographic filters suitable for image improvement

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100108271 ◽

1978 ◽

Vol 36 (1) ◽

pp. 226-227

Author(s):

K.-H. Herrmann ◽

E. Reuber ◽

P. Schiske

Keyword(s):

Transfer Functions ◽

Diffraction Order ◽

Lateral Position ◽

Simple Method ◽

Spatial Frequencies ◽

Resolution Electron ◽

Wiener Filters ◽

Image Improvement ◽

Optical Reconstruction ◽

Set Up

Aposteriori deblurring of high resolution electron micrographs of weak phase objects can be performed by holographic filters [1,2] which are arranged in the Fourier domain of a light-optical reconstruction set-up. According to the diffraction efficiency and the lateral position of the grating structure, the filters permit adjustment of the amplitudes and phases of the spatial frequencies in the image which is obtained in the first diffraction order.In the case of bright field imaging with axial illumination, the Contrast Transfer Functions (CTF) are oscillating, but real. For different imageforming conditions and several signal-to-noise ratios an extensive set of Wiener-filters should be available. A simple method of producing such filters by only photographic and mechanical means will be described here.A transparent master grating with 6.25 lines/mm and 160 mm diameter was produced by a high precision computer plotter. It is photographed through a rotating mask, plotted by a standard plotter.

Download Full-text

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099192 ◽

1981 ◽

Vol 39 ◽

pp. 550-551

Author(s):

Dean A. Handley ◽

Jack T. Alexander ◽

Shu Chien

Keyword(s):

Cell Growth ◽

Cell Cultures ◽

Molecular Interactions ◽

Convenient Method ◽

Petri Dish ◽

Simple Method ◽

Thin Section Microscopy ◽

Thin Sectioning ◽

Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

Download Full-text