scholarly journals Gas-liquid chromatography of carbohydrate derivatives: the separation and quantitative determination of synthetic and naturally occurring glycosyl derivatives of amino acids

1972 ◽  
Vol 22 (2) ◽  
pp. 369-379 ◽  
Author(s):  
George W. Jourdian ◽  
Jack J. Distler
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Gas Liquid Chromatography ◽  
Naturally Occurring ◽  
Derivatives Of

scholarly journals Determination of asparagine, glutamine and pyrrolidonecarboxylic acid in total enzymic hydrolysates of peptides and glycopeptides by gas–liquid chromatography

1973 ◽  
Vol 133 (3) ◽  
pp. 551-561 ◽  
Author(s):  
Hedy Hediger ◽  
Richard L. Stevens ◽  
Hans Brandenberger ◽  
Karl Schmid
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Room Temperature ◽  
Butyl Ester ◽  
Gas Liquid Chromatography ◽  
Qualitative And Quantitative ◽  
Elution Pattern

A new procedure for the qualitative and quantitative determination of asparagine, glutamine and pyrrolidonecarboxylic acid in total enzymic hydrolysates of peptides and glycopeptides based on g.l.c. has been developed. Under the conditions of esterification and trifluoroacetylation N-trifluoroacetylaspartic acid mono-n-butyl ester was formed from asparagine and N-trifluoroacetylglutamic acid mono-n-butyl ester from both glutamine and pyrrolidonecarboxylic acid. To distinguish between the latter two compounds, the esterification was carried out at room temperature yielding 30% of esterified pyrrolidonecarboxylic acid but less than 1% of esterified glutamine. In extending the g.l.c. of amino acids, the previously unknown positions in the g.l.c. elution pattern of the following amino acids could also be reproducibly determined: carboxymethylcysteine, homoserine, hydroxylysine and ∈-methyl-lysine. Further, certain glycopeptides were investigated and the artifacts due to their carbohydrate moieties were determined.

DETERMINATION OF PLASMA OESTRIOL IN NORMAL PREGNANCY AND LABOUR USING GAS-LIQUID CHROMATOGRAPHY

1971 ◽  
Vol 51 (3) ◽  
pp. 447-454 ◽  
Author(s):  
W. COOPER ◽  
M. G. COYLE ◽  
J. A. MILLS
Keyword(s):  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Internal Standard ◽  
Normal Pregnancy ◽  
Gas Liquid Chromatography ◽  
Chemical Purification ◽  
Peripheral Plasma ◽  
Pregnancy And Delivery

SUMMARY A method is described for estimating oestriol in 2–10 ml samples of human pregnancy peripheral plasma. It incorporates acid hydrolysis, chemical purification, methylation, chromatography on alumina columns, formation of a derivative and quantitative determination by gas chromatography. A radioactive internal standard was added to correct for procedural losses. Plasma oestriol determinations in five normal patients throughout pregnancy and delivery are reported.

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