The kinetics of HIV-1 long terminal repeat transcriptional activation resemble those of hsp70 promoter in heat-shock treated HeLa cells

FEBS Letters ◽  
1994 ◽  
Vol 351 (2) ◽  
pp. 191-196 ◽  
Author(s):  
Carole Kretz-Remy ◽  
Andre-Patrick Arrigo
Keyword(s):  
Heat Shock ◽  
Long Terminal Repeat ◽  
Hela Cells ◽  
Transcriptional Activation ◽  
Terminal Repeat ◽  
Hsp70 Promoter ◽  
Kinetics Of ◽  
Hiv 1

scholarly journals Functional domains required for tat-induced transcriptional activation of the HIV-1 long terminal repeat.

1988 ◽  
Vol 7 (10) ◽  
pp. 3143-3147 ◽  
Author(s):  
J. A. Garcia ◽  
D. Harrich ◽  
L. Pearson ◽  
R. Mitsuyasu ◽  
R. B. Gaynor
Keyword(s):  
Long Terminal Repeat ◽  
Transcriptional Activation ◽  
Terminal Repeat ◽  
Functional Domains ◽  
Hiv 1

Transcriptional Activation of the Human Immunodeficiency Virus Long Terminal Repeat Sequences by Retinoic Acid in Human Epithelial and Fibroblast Tumor Cell Lines

1996 ◽  
Vol 11 (3) ◽  
pp. 153-158 ◽  
Author(s):  
V. Zoumpourlis ◽  
M. Ergazaki ◽  
D.A. Spandidos
Keyword(s):  
Retinoic Acid ◽  
Tumor Cell ◽  
Long Terminal Repeat ◽  
Cell Lines ◽  
Transcriptional Activation ◽  
Critical Role ◽  
Terminal Repeat ◽  
Tumor Cell Lines ◽  
A Cells ◽  
Hiv 1

We employed a recombinant plasmid, pBHIV1, carrying the long terminal repeat (LTR) sequences of HIV-1 linked to the reporter chloramphenicol acetyl transferase (CAT) gene and to the aminoglycoside phosphotransferase (aph) gene as a selectable marker. We introduced pBHIV1 into human epithelial and fibroblast tumor cell lines (HeLa and MRCSV40TGR), and obtained stable geneticin-resistant HLHIV1-A and SVTGHIV1-A cells, respectively. The response to the retinoic acid was studied on the LTR regulated CAT activity in both cell lines. It was found that retinoic acid at a concentration of 1×10−5 effects a 3.2 - fold increase in CAT expression compared to HIV LTR in HLHIV1-A, but requires a concentration of 5×10−5 M to enhance this expression 4.6-fold in SVTGHIV1-A cells. These data show that retinoic acid may play a critical role in HIV-1 expression in human epithelial and fibroblast cell lines.

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