Simultaneous high performance liquid chromatographic and derivative ratio spectra spectrophotometry determination of chlorpheniramine maleate and phenylephrine hydrochloride

Il Farmaco ◽  
1998 ◽  
Vol 53 (8-9) ◽  
pp. 617-622 ◽  
Author(s):  
Nevin Erk ◽  
Murat Kartal
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Liquid Chromatographic

scholarly journals Simultaneous Determination of Phenylephrine Hydrochloride, Guaifenesin, and Chlorpheniramine Maleate in Cough Syrup by Gradient Liquid Chromatography

2008 ◽  
Vol 91 (2) ◽  
pp. 276-284 ◽  
Author(s):  
Sawsan M Amer ◽  
Samah S Abbas ◽  
Mostafa A Shehata ◽  
Nahed M Ali
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Pure Form ◽  
Chlorpheniramine Maleate ◽  
Phenylephrine Hydrochloride ◽  
Cough Syrup ◽  
Liquid Chromatographic

Abstract A simple and reliable high-performance liquid chromatographic method was developed for the simultaneous determination of mixture of phenylephrine hydrochloride (PHENYL), guaifenesin (GUAIF), and chlorpheniramine maleate (CHLO) either in pure form or in the presence of methylparaben and propylparaben in a commercial cough syrup dosage form. Separation was achieved on a C8 column using 0.005 M heptane sulfonic acid sodium salt (pH 3.4 0.1) and acetonitrile as a mobile phase by gradient elution at different flow rates, and detection was done spectrophotometrically at 210 nm. A linear relationship in the range of 30180, 1201800, and 1060 g/mL was obtained for PHENYL, GUAIF, and CHLO, respectively. The results were statistically analyzed and compared with those obtained by applying the British Pharmacopoeia (2002) method and showed that the proposed method is precise, accurate, and can be easily applied for the determination of the drugs under investigation in pure form and in cough syrup formulations.

scholarly journals Simultaneous Determination of Paracetamol, Phenylephrine Hydrochloride, Oxolamine Citrate and Chlorpheniramine Maleate by HPLC in Pharmaceutical Dosage Forms

2011 ◽  
Vol 8 (3) ◽  
pp. 1275-1279 ◽  
Author(s):  
Ozan Pirol ◽  
Murat Sukuroglu ◽  
Tuncel Ozden
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Dosage Forms ◽  
Control Analysis ◽  
Chlorpheniramine Maleate ◽  
Pharmaceutical Dosage Forms ◽  
Phenylephrine Hydrochloride ◽  
Relative Standard ◽  
Liquid Chromatographic

A new high performance liquid chromatographic (HPLC) method was developed for the determination of paracetamol, phenylephrine hydrochloride, oxolamine citrate and chlorpheniramine maleate in combined pharmaceutical formulations and dosage forms. The separation was performed on an Agilent Zorbax SB-CN column with the mobile phase consisting of 0.02 M phosphate buffer (pH:4) and acetonitrile (85:15,v/v) in flow rate 1.5 mL at 22 °C. The overall retention time of the analytes was 3.5 min. The method was validated with respect to linearity, precision, accuracy and recovery. The relative standard deviation for 10 replicate measurements of paracetamol, phenylephrine HCl, oxolamine citrate and chlorpheniramine maleate were 0.12, 0.36 0.18 and 0.59%, respectively. Total recoveries of analytes were 99.99, 100.56, 100.20 and 99.60%, respectively. No chromatographic interference from the tablet excipients was found. The linearity of paracetamol, phenylephrine HCl, oxolamine citrate and chlorpheniramine maleate were in the range of 20-120 μg/mL, 0.4-2.4 μg/mL, 8-48 μg/mL and 0.16-0.96 μg/mL, respectively. This simple, fast, economical and precise high performance liquid chromatographic method can be adopted for routine quality control analysis.

scholarly journals Development and Validation of an RP-HPLC Method for Estimation of Chlorpheniramine Maleate, Ibuprofen, and Phenylephrine Hydrochloride in Combined Pharmaceutical Dosage Form

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Pinak M. Sanchaniya ◽  
Falgun A. Mehta ◽  
Nirav B. Uchadadiya
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Hplc Method ◽  
Chlorpheniramine Maleate ◽  
Pharmaceutical Dosage Form ◽  
Form Analysis ◽  
Phenylephrine Hydrochloride ◽  
Liquid Chromatographic

The objective of this paper is to develope a simple, precise, accurate, and reproducible reversed phase high performance liquid chromatographic method for the quantitative determination of chlorpheniramine maleate, ibuprofen, and phenylephrine hydrochloride in combined pharmaceutical dosage form. Analysis was carried out using acetonitrile : mathanol : phoshphate buffer (50 : 20 : 30, v/v/v, pH 5.6) mobile phase at 1.0 mL/min flow rate and Sunfire C 18 column (5 μm × 250 mm × 4.6 mm) as stationary phase with detection wavelength of 220 nm. The retention times of chlorpheniramine maleate (CPM), ibuprofen (IBU), and phenylephrine hydrochloride (PHE) were 4.2 min, 13.6 min, and 2.7 min, respectively. The proposed method was validated with respect to linearity, accuracy, precision, specificity, and robustness. The linearity for chlorpheniramine maleate, ibuprofen, and phenylephrine hydrochloride was in the range of 0.5–2.5 μg/mL, 25–125 μg/mL, and 1.25–6.25 μg/mL, respectively. The % recoveries of all the three drugs were found to be 99.44–101.61%, 99.39–101.79%, and 98.66–101.83%. LOD were found to be 32, 120, and 68 ng/mL for CPM, IBU, and PHE, respectively. The method was successfully applied to the estimation of chlorpheniramine maleate, ibuprofen, and phenylephrine hydrochloride in combined pharmaceutical dosage form.

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