Changes in the Protein Conformation of Human Spermatozoal Membranes after Treatment with Cyclic Adenosine 3′ :5′ -Monophosphate and Human Follicular Fluid

1976 ◽  
Vol 27 (4) ◽  
pp. 413-420 ◽  
Author(s):  
Néstor M. Delgado ◽  
Luis Huacuja ◽  
Rosa Ma. Pancardo ◽  
Horacio Merchant ◽  
Adolfo Rosado
1987 ◽  
Vol 116 (3_Suppl) ◽  
pp. S117-S118
Author(s):  
H. JARRY ◽  
B. MEYER ◽  
G. HOLZAPFEL ◽  
B. HINNEY ◽  
D. TEMME ◽  
...  

Zygote ◽  
2021 ◽  
pp. 1-5
Author(s):  
H. Debbarh ◽  
N. Louanjli ◽  
S. Aboulmaouahib ◽  
M. Jamil ◽  
L. Ahbbas ◽  
...  

Summary Maternal age is a significant factor influencing in vitro fertilization (IVF) outcomes. Oxidative stress (OS) is one of the major causes of age-related cellular and molecular damage. The purpose of this work was to investigate the correlation between maternal age with intrafollicular antioxidants and OS markers in follicular fluid (FF), and also to determine the OS status in patients of advanced age. This study was a prospective study including 201 women undergoing IVF whose age was between 24 and 45 years old. FF samples were obtained from mature follicles at the time of oocyte retrieval. After treatment of FF, lipid peroxidation levels (MDA) and enzyme activities such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione (GSH) level were evaluated using spectrophotometry. The results indicated that the age cutoff point for increasing the MDA level was fixed at 37 years, allowing the study to be differentiated into two age groups. Group I included patients whose age was less than 37 years, and group II included patients whose age was greater than or equal 37 years. Statistical analysis revealed that MDA and GSH levels and GR activity were significantly higher in group II compared with group I. The SOD and CAT activities were significantly less in group II compared with group I. We concluded that from 37 years old a reproductive ageing was accompanied by a change in the antioxidant pattern in FF that impaired reactive oxygen species scavenging efficiency.


1987 ◽  
Vol 48 (3) ◽  
pp. 514 ◽  
Author(s):  
Paul G. McDonough ◽  
P.J. Taylor ◽  
M.J. Gill ◽  
M. Mahadevan ◽  
H.A. Pattinson

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Rosie M. Martinez ◽  
Liming Liang ◽  
Catherine Racowsky ◽  
Laura Dioni ◽  
Abdallah Mansur ◽  
...  

2021 ◽  
Vol 9 (B) ◽  
pp. 1745-1750
Author(s):  
Maanee Azzam ◽  
Adeela Hamood ◽  
Hind Abdulkadim

Background: Follicular fluid considered as an important microenvironment for oocyte development, cell free-DNA (cfDNA) fragments that are found in this fluid and are released from cell apoptosis and/or necrosis, aimed to quantified the level of cf-DNA, in the follicular fluid and to assess any relation between the level of cf-DNA in this fluid with women’s age, duration of infertility, cause of infertility, her ovarian reserve values. Methods: Eighty-nine women were prospectively included in this study FF cf-DNA which was determined by conventional real time PCR-syber green detection approach which quantified by ALU-specific primers. Results: cell-free DNA (cfDNA) level in Follicular fluid samples of Iraqi women level was; cfDNA (Mean±SD, 0.916±0.106 ng/μl). there was no significant relation between cfDNA and pregnancy outcome, but very low level and very high level cf DNA were related to negative pregnancy outcome, cfDNA was second most important predictive factor of pregnancy outcome after fertilization rate, but both not statistically significant p value was (0.622 and 0.241) respectively. Conclusion: current study notice that cfDNA in the follicular fluid may mainly reflect the cellular activity and the balance between programed apoptosis and cell necrosis.


1988 ◽  
Vol 20 (04) ◽  
pp. 243-246 ◽  
Author(s):  
Ch. Lindner ◽  
V. Lichtenberg ◽  
G. Westhof ◽  
W. Braendle ◽  
G. Bettendorf

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