Metallothionein isoforms mRNA expression after cisplatin treatment in human tongue carcinoma cell lines

Abstract Background This study aimed to investigate the expression level of the GATA6 gene in different oral cancer cells. Methods In this study, we sub-cultured normal oral epithelial cell lines HOK, human tongue squamous cell carcinoma cell lines CAL-27 and SCC-4, and human salivary gland adenoid cystic carcinoma cell lines SACC-LM and SACC-83. Subsequently, we used reverse transcription-polymerase chain reaction RT-PCR and Western blot methods to detect the mRNA and the protein expressions of GATA6 in normal oral epithelial cells, human tongue squamous cell carcinoma cells, and human salivary gland adenoid cystic carcinoma cells. Results The results of this study showed that the mRNA expression levels of GATA6 in CAL-27, SCC-4, and SACC-LM cells were significantly increased when compared with the HOK cells. However, the mRNA expression level of GATA6 in the SACC-83 cells had no significant difference compared with the HOK cells. The protein expression levels of GATA6 in the SCC-4 and SACC-LM cells were, however, significantly increased whereas the protein expression levels of GATA6 in the CAL-27 and SACC-83 cells had no significant difference when compared with the HOK cells. Conclusion The GATA6 gene may be related to the occurrence and progression of certain oral cancers.

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Part II. Initial molecular and cellular characterization of high nitric oxide-adapted human tongue squamous cell carcinoma cell lines

Tumor Biology ◽

10.1007/s13277-010-0102-0 ◽

2010 ◽

Vol 32 (1) ◽

pp. 87-98 ◽

Cited By ~ 7

Author(s):

Gabor Tarjan ◽

G. Kenneth Haines ◽

Benjamin J. Vesper ◽

Jiaping Xue ◽

Michael B. Altman ◽

...

Keyword(s):

Nitric Oxide ◽

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Cell Lines ◽

Squamous Cell ◽

Carcinoma Cell ◽

Squamous Cell Carcinoma Cell ◽

Carcinoma Cell Lines ◽

Human Tongue

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Identifying cancer-related genes in nasopharyngeal carcinoma cell lines using DNA and mRNA expression profiling analyses

International Journal of Oncology ◽

10.3892/ijo.21.6.1197 ◽

2002 ◽

Cited By ~ 1

Author(s):

Xiang Guo ◽

Weng-Onn Lui ◽

Chao-Nan Qian ◽

Jin-Dong Chen ◽

Steven Gray ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Mrna Expression ◽

Cell Lines ◽

Expression Profiling ◽

Carcinoma Cell ◽

Nasopharyngeal Carcinoma Cell ◽

Carcinoma Cell Lines ◽

Mrna Expression Profiling

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ppENKGene Methylation Status in the Development of Pancreatic Carcinoma

Gastroenterology Research and Practice ◽

10.1155/2013/130927 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 5

Author(s):

Lixin Yang ◽

Hong Yang ◽

Jingnan Li ◽

Jianyu Hao ◽

Jiaming Qian

Keyword(s):

Pancreatic Cancer ◽

Mrna Expression ◽

Pancreatic Carcinoma ◽

Cell Lines ◽

Carcinoma Cell ◽

Methylation Status ◽

Pancreatic Tissue ◽

Human Pancreatic Cancer ◽

Normal Pancreatic Tissue ◽

Carcinoma Cell Lines

Objective. To explore the association of hypermethylation of the proenkephalin gene (ppENK) with pancreatic carcinoma and to identify the effects of a demethylating agent on pancreatic cell lines.Method. Human pancreatic cancer tissues and five pancreatic carcinoma cell lines, as well as normal pancreatic tissue, were used.ppENKmethylation status was detected by MS-PCR (methylation-specific PCR).Results. Methylation ofppENKwas detected in 90.3% (28/31) of the human pancreatic carcinoma tissues but was not seen in normal pancreatic tissue. There was no correlation between the extent of methylation ofppENKand the clinicopathological features of the pancreatic carcinomas. MethylatedppENKwas detected in all the pancreatic cancer cell lines and was associated with loss of mRNA expression in the pancreatic carcinoma cell lines and normal pancreatic tissue. After treatment with 5-aza-dC, methylatedppENKwas not detected and the inhibition ofppENKmRNA expression was reversed.Conclusions. Inhibition ofppENKexpression by a change in its methylation status plays an important role in pancreatic carcinogenesis.ppENKmethylation is thus an important molecular event that distinguishes pancreatic carcinoma tissue from normal pancreatic tissue. Effects on cell growth, apoptosis, and the cell cycle may contribute to changes ofppENKmethylation status.

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Model of human tongue squamous cell line stably transfected with human papillomavirus（HPV) 16 E6 and E7 genes and biological characteristic analysis

10.21203/rs.3.rs-124593/v1 ◽

2020 ◽

Author(s):

Zilian Lan ◽

Ziyao Jia ◽

Hengyuan Guo ◽

Zhaoshou Yang ◽

Zifan Yang ◽

...

Keyword(s):

Cell Lines ◽

Carcinoma Cell ◽

Squamous Carcinoma ◽

Migration Ability ◽

Carcinoma Cell Lines ◽

Human Tongue ◽

Squamous Carcinoma Cell ◽

And Migration ◽

Tongue Squamous Carcinoma ◽

Proliferation And Migration

Abstract Human tongue squamous carcinoma cell lines transfected with HPV16E6E7 gene were established to provide a model for further study of HPV16 E6E7-related human tongue squamous carcinoma cell lines .Plasmid pEGFR/HPV16 of E6E7 and plasmid pEGFR/HPV16 of No E6E7 were constructed.Human tongue squamous carcinoma cell lines including SCC9 and SCC15 were infected by liposome transfection and would be highly selected by antibiotic .Fluorescence imaging, RT-PCR and Westernblot were used to detect the expression of HPV16 E6E7 in cells.The biological characteristics of human tongue squamous carcinoma cell lines infected with HPV16 E6E7 were detected by CCK-8 and wound healing assay. The human tongue squamous carcinoma cell lines transfected with HPV16 E6E7 gene were successfully established and identified, and the proliferation and migration ability of the human tongue squamous carcinoma cell lines infected with HPV16 E6E7 gene was significantly stronger than that of the blank group.Human tongue squamous carcinoma cell lines infected with HPV16 E6E7 were more malignant, and their proliferation and migration ability were higher than those not infected with HPV16 E6E7.

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