scholarly journals Effects of hypothyroidism on ovarian hormone secretion and follicular development in immature female rats

1997 ◽  
Vol 73 ◽  
pp. 103
Author(s):  
Minoru Hatsula ◽  
Kazuhiro Tamura ◽  
Gen Watanabe ◽  
Kazuyoshi Taya ◽  
Hiroshi Kogo Kogo
Keyword(s):  
Hormone Secretion ◽  
Follicular Development ◽  
Female Rats ◽  
Ovarian Hormone ◽  
Immature Female

scholarly journals Major Royal Jelly Proteins Accelerate Onset of Puberty and Promote Ovarian Follicular Development in Immature Female Mice

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1145-1145
Author(s):  
Lirong Shen ◽  
Xin Liu ◽  
Yong Chen ◽  
Fangxiong Shi ◽  
Chao-Qiang Lai
Keyword(s):  
Reproductive System ◽  
Royal Jelly ◽  
Hormone Secretion ◽  
Follicular Development ◽  
Serum Estradiol ◽  
Immature Female ◽  
Female Mice ◽  
Estrogenic Effect ◽  
Ovarian Follicular Development ◽  
Estradiol Levels

Abstract Objectives Estrogen plays an important role in for growth and development of reproductive system in female. The major royal jelly (RJ) has a significant estrogenic-like effect for many female animals. The major RJ proteins (MRJPs) are the central constituents responsible for the physiological activities of RJ. In recent years, we have found that MRJPs possesses functions to increase fecundity associated with estrogenic effect in Drosophila. However, if MRJPs are the specific active ingredient mediating estrogenic effect of RJ and related action mechanism in mammalians remains unclear. Methods Neonatal immature female mice (FC) were divided into four groups fed MRJPs with doses of 0, 125, 250 and 500 mg/kg/body weight (M125, M250 and M500), respectively. The freeze-dried MRJPs powder was administrated daily for 45 consecutive days. The effects of MRJPs on the puberty onset, ovarian follicular development and serum estradiol levels in FC were evaluated. Results The times of estrus in M125, M250 and M500 were accelerated by 10.7%, 15.5% and 10.7%, the ovarian index of M125 and M250 and M500 were increased by 26.8% and 32.1% and 1.7%. The number of secondary follicles in M125 and M250 and M500 were increased by 50.7%,78.8% and 38.6%, the Graafian follicles in M125 and M250 and M500 were increased by 600.0% and 774.0% and 150.0%. M500 induced multi-oocyte follicles. The serum estradiol levels of M125, M250 and M500 were increased by 47.1%, 64.9% and 31.1%, the action of MRJPs raising hormone secretion level is mainly via upregulating expression of ERβ gene. Antioxidant parameters of ovarian tissue showed that the malondialdehyde levels in M125 and M250 were decreased, the superoxide dismutase activities and glutathione peroxidase activities in M125 and M250 were increased, respectively. Conclusions Oral administration of MRJPs may accelerate onset of puberty and promote follicular development in immature FM. The reproductive performance promotion of MRJPs was associated with raising estrogenic activity and antioxidant potential to reproductive system, upregulating expression of ERβ gene to raise hormone secretion and promote ovary development in FM. Funding Sources The National Natural Science Foundation of China and US Department of Agriculture, Agriculture Research Service.

scholarly journals Effects of reduction of the number of primordial follicles on follicular development to achieve puberty in female rats

Reproduction ◽  
2003 ◽  
pp. 85-94 ◽  
Author(s):  
M Shirota ◽  
S Soda ◽  
C Katoh ◽  
S Asai ◽  
M Sato ◽  
...  
Keyword(s):  
Corn Oil ◽  
Follicular Development ◽  
Primary Phase ◽  
Female Rats ◽  
Female Rat ◽  
Serum Concentrations ◽  
Immature Female ◽  
Primordial Follicles ◽  
Preantral Follicles ◽  
Rat Offspring

Effects of reduction of the number of primordial follicles on follicular development and concentrations of circulating hormones were examined in immature female rat offspring of dams given busulfan intraperitoneally on day 14 of gestation. The offspring of dams treated with 5 mg busulfan kg(-1) showed vaginal opening at an age comparable with the offspring of dams treated with 2.5 mg busulfan kg(-1) or with corn oil as a control, although they exhibited an irregular oestrous cycle until week 14 after birth. The serum concentrations of immunoreactive inhibin and FSH on day 26 after birth of the offspring treated with 5 mg busulfan kg(-1) were similar to those of age-matched controls. On day 15 after birth, however, the concentration of their immunoreactive inhibin was markedly lower than that of controls, whereas the concentration of their FSH was increased inversely. Comparison of the numbers of ovarian follicles in the controls and groups treated with 2.5 mg busulfan kg(-1) and 5 mg busulfan kg(-1) revealed that prenatal treatment with busulfan reduced the number of follicles in the primordial or primary phase and in the preantral phase on day 7 after birth. Although the increase of the ratio of the number of preantral follicles during days 7-13 after birth tended to vary with the prenatal dose of busulfan, the number of preantral follicles in the group treated with 5 mg busulfan kg(-1) was still smaller than in the controls. The concentration of serum immunoreactive inhibin of the offspring treated with busulfan was reduced on day 7 after birth without alteration of the concentration of gonadotrophin. On day 13 after birth, the concentration of serum immunoreactive inhibin was reduced only in the offspring treated with 5 mg busulfan kg(-1), and the concentration of serum FSH of the offspring was increased inversely as found on day 15 after birth. These results indicate that a reduction in the number of primordial follicles decreases the number of follicles that enter the growing phase, a major source of circulating inhibin in the neonatal and infantile ovary, and that consequently increased circulating FSH may accelerate follicular development to achieve puberty.

scholarly journals Effect of direct ovarian injection of vascular endothelial growth factor gene fragments on follicular development in immature female rats

Reproduction ◽  
2007 ◽  
Vol 134 (5) ◽  
pp. 677-682 ◽  
Author(s):  
Takashi Shimizu ◽  
Koji Iijima ◽  
Kanako Miyabayashi ◽  
Yoshinori Ogawa ◽  
Hitoshi Miyazaki ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Follicular Development ◽  
Female Rats ◽  
Vascular Endothelial ◽  
Vegf Gene ◽  
Immature Female ◽  
Rat Ovary ◽  
Ovulatory Follicles ◽  
Endothelial Growth Factor

Vascular endothelial growth factor (VEGF) expression in granulosa cells is associated with the thecal vasculature growth during ovarian follicular development. We hypothesized that injection of VEGF gene fragments directly into the rat ovary would induce production of a large number of ovulatory follicles and that these follicles would ovulate. To test this hypothesis, we treated immature female rats with combinations of hormones and VEGF gene fragments. The animals were divided into two groups: one group received solution containing transfection reagents as a control (n= 5), while the other group received direct ovarian injection of VEGF gene fragments at 19 (n= 5), 21 (n= 5), 23 (n= 5), or 25 (n= 5) days after birth followed by i.p. administration of 20 IU equine chorionic gonadotropin (eCG) at the age of 26 days. Forty-eight hours after eCG injection, animals were given 20 IU human chorionic gonadotropin (hCG) i.p. and then the oocytes in both groups were counted. The maximum number of ovulated oocytes was obtained when the VEGF gene fragments were injected into the rat ovary at 21 days after birth. Histological examination revealed that the injection of VEGF gene fragments markedly increased the vascular density around the preovulatory follicles and also the number of these follicles. Our data provide the first reported evidence that most ovulatory follicles generated by injection of VEGF gene fragments are able to ovulate upon hCG treatment. These results demonstrate that injection of VEGF gene fragments directly into the ovary stimulates the development of antral follicles by inducing the formation of thecal vasculature in immature female rats.

Changes in inhibin-A (α-βA dimer) and total α inhibin in the peripheral circulation and ovaries of rats after gonadotrophin-induced follicular development and during the normal oestrous cycle

1995 ◽  
Vol 147 (2) ◽  
pp. 271-283 ◽  
Author(s):  
P A Fahy ◽  
C A Wilson ◽  
A J Beard ◽  
N P Groome ◽  
P G Knight
Keyword(s):  
Plasma Concentrations ◽  
Follicular Development ◽  
Serum Levels ◽  
Peripheral Circulation ◽  
Oestrous Cycle ◽  
Female Rats ◽  
Immature Female ◽  
Adult Rats ◽  
Inhibin A ◽  
Α Subunit

Abstract Recent modifications to a previously reported two-site IRMA have permitted the measurement of serum/plasma concentrations and ovarian contents of inhibin-A (α-βA dimer) in pregnant mare serum gonadotrophin (PMSG)treated immature female rats and adult rats throughout the 4-day oestrous cycle. For comparison, total α inhibin levels were also measured by α subunit-directed inhibin RIA and found to be at least tenfold higher (relative to the same 32 kDa bovine inhibin standard used to calibrate both assays). In immature female rats, serum levels of inhibin-A dimer and total α inhibin increased within 3 h of PMSG injection and rose in parallel over the next 48 h to values four- to fivefold higher than pretreatment levels. Ovariectomy led to a rapid and parallel fall in both inhibin-A dimer and total α inhibin; initial half-lives (±95% confidence intervals) were 22 ± 4 and 20 ± 5 min respectively. In adult rats, marked fluctuations in plasma concentrations and ovarian contents of inhibin-A dimer and total α inhibin occurred during the 4-day oestrous cycle, most notably between the morning of pro-oestrus and the morning of oestrus. Plasma levels of inhibin-A dimer and total α inhibin peaked on the afternoon of pro-oestrus just before the preovulatory gonadotrophin surge. After ovulation, both inhibin-A dimer and total α inhibin fell abruptly (two- to threefold by 0200 h on oestrus; P<0·001), while FSH showed a secondary rise which peaked at 0700 h on oestrus. Although IRMA- and RIA-derived inhibin values generally followed a similar pattern across the 4-day cycle (plasma: r=0·52, P<0·001; ovary: r=0·41, P<0·001), a transient rise in plasma and ovarian inhibin-A dimer was detected at 0700 h on oestrus (P<0·01) which was unaccompanied by a rise in total α inhibin. This rise in plasma inhibin-A dimer was probably responsible for terminating the post-ovulatory FSH surge since FSH levels declined steadily over the next 15 h. Overall, plasma inhibin-A dimer and FSH concentrations across the whole cycle were negatively correlated (r= −0·22, P<0·01) whereas no correlation existed between total α inhibin and FSH (r= −0·11, P=0·12). Journal of Endocrinology (1995) 147, 271–283

NEUTRALIZATION OF ACTIVITY OF CIRCULATING GONADOTROPHIC HORMONES BY ANTISERUM TO RAT PITUITARY

1963 ◽  
Vol 25 (4) ◽  
pp. 451-456 ◽  
Author(s):  
A. N. CONTOPOULOS ◽  
T. HAYASHIDA
Keyword(s):  
Serum Proteins ◽  
Follicular Development ◽  
Inhibitory Effect ◽  
Female Rats ◽  
Male Rats ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Hormone Activity ◽  
Immature Female ◽  
Rat Pituitary

SUMMARY Antiserum prepared by the immunization of rabbits, with homogenates of rat anterior pituitary gland according to a procedure previously outlined, was absorbed with homologous serum proteins and tissues to remove various non-specific antibodies. Varying levels of plasma from gonadectomized male rats, containing high levels of gonadotrophic hormone activity, were injected into hypophysectomized, immature female rats. The simultaneous injection of antiserum resulted in complete neutralization of gonadotrophic hormone as judged by the inhibition of ovarian and uterine weight responses and the extent of follicular development in the ovaries of the rats used for the bioassay. The degree of inhibition was dependent upon the relative amount of antiserum employed. Normal rabbit serum did not have any inhibitory effect. No detectable non-specific or toxic effects were noted in the animals injected with antiserum.

THE EFFECTS OF TREATMENT WITH GONADOTROPHINS OR WITH OESTROGEN ON THE THYROID GLAND OF THE IMMATURE RAT

1966 ◽  
Vol 35 (3) ◽  
pp. 263-270 ◽  
Author(s):  
K. BROWN-GRANT
Keyword(s):  
Thyroid Gland ◽  
Hormone Secretion ◽  
Thyroid Stimulating Hormone ◽  
Female Rats ◽  
Male Rats ◽  
Luteinizing Hormone Secretion ◽  
Immature Female ◽  
Immature Rat ◽  
Tsh Secretion ◽  
Pregnant Mare Serum Gonadotrophin

SUMMARY The uptake of 131I by the thyroid gland increased 72 hr. after the injection of pregnant mare serum gonadotrophin (PMS) into immature female rats whether ovulation occurred or not. PMS failed to produce this effect in male rats but oestrogen administration increased 131I uptake in both male and female immature rats, suggesting that oestrogen was responsible for the effect of PMS in females. Both PMS and oestrogen may increase the uptake of radioactive phosphate by the thyroid of female but not male rats; oestrogen may stimulate thyroid-stimulating hormone (TSH) secretion in immature female rats. These effects of oestrogen in the female made it impossible to determine whether the 'ovulatory surge' in luteinizing hormone secretion in PMS-treated rats was associated with an increased secretion of TSH or not.

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