scholarly journals Microenvironment of two different extrinsic fluorescence probes in Na+,K+-ATPase changes out of phase during sequential appearance of reaction intermediates.

1988 ◽  
Vol 263 (26) ◽  
pp. 12943-12947 ◽  
Author(s):  
K Taniguchi ◽  
H Tosa ◽  
K Suzuki ◽  
Y Kamo
Keyword(s):  
Fluorescence Probes ◽  
Reaction Intermediates ◽  
Sequential Appearance

Analyzing reactions of single mechanoenzyme molecules by light microscopy

1992 ◽  
Vol 50 (1) ◽  
pp. 426-427
Author(s):  
Jeff Gelles
Keyword(s):  
Image Processing ◽  
Reaction Mechanisms ◽  
Transient State ◽  
Nanometer Scale ◽  
Reaction Intermediates ◽  
Enzyme Molecule ◽  
Directed Movement ◽  
Enzyme Molecules ◽  
Individual Enzyme ◽  
Single Reaction

Mechanoenzymes are enzymes which use a chemical reaction to power directed movement along biological polymer. Such enzymes include the cytoskeletal motors (e.g., myosins, dyneins, and kinesins) as well as nucleic acid polymerases and helicases. A single catalytic turnover of a mechanoenzyme moves the enzyme molecule along the polymer a distance on the order of 10−9 m We have developed light microscope and digital image processing methods to detect and measure nanometer-scale motions driven by single mechanoenzyme molecules. These techniques enable one to monitor the occurrence of single reaction steps and to measure the lifetimes of reaction intermediates in individual enzyme molecules. This information can be used to elucidate reaction mechanisms and determine microscopic rate constants. Such an approach circumvents difficulties encountered in the use of traditional transient-state kinetics techniques to examine mechanoenzyme reaction mechanisms.

Lateral Adsorbate Interactions Inhibit HCOO- While Promoting CO Selectivity for CO2 Electrocatalysis on Ag

2018 ◽  
Author(s):  
Divya Bohra ◽  
Isis Ledezma-Yanez ◽  
Guanna Li ◽  
Wiebren De Jong ◽  
Evgeny A. Pidko ◽  
...  
Keyword(s):  
Electrochemical Reduction ◽  
Experimental Approach ◽  
Decisive Role ◽  
Experimental Methods ◽  
Reaction Intermediates ◽  
Intermediate Species ◽  
Experimental Knowledge ◽  
Complex Interactions ◽  
Ag Catalysts

<p>The analysis presented in this manuscript helps bridge an important fundamental discrepancy between the existing theoretical and experimental knowledge regarding the performance of Ag catalysts for CO<sub>2</sub> electrochemical reduction (CO<sub>2</sub>ER). The results demonstrate how the intermediate species *OCHO is formed readily en-route the HCOO<sup>– </sup>pathway and plays a decisive role in determining selectivity of a predominantly CO producing catalyst such as Ag. Our theoretical and experimental approach develops a better understanding of the nature of competition as well as the complex interactions between the reaction intermediates leading to CO, HCOO<sup>–</sup> and H<sub>2</sub> during CO<sub>2</sub>ER.</p><p><br></p><p>Details of computational and experimental methods are present in the Supporting Information provided. </p><p><br></p><p><br></p>

Lanthanide-doped Fluorescence Probes for NIR-Ⅱ Fluorescence Imaging

2020 ◽  
Vol 41 (12) ◽  
pp. 1460-1478
Author(s):  
Song-tao ZHANG ◽  
◽  
Ying-hui WANG ◽  
Hong-jie ZHANG ◽  
◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Probes

Recent Progress in Chemosensors Using Aldehyde-bearing Fluorophores for the Detection of Specific Analytes and their Bioimaging

2019 ◽  
Vol 26 (21) ◽  
pp. 4003-4028 ◽  
Author(s):  
Fangjun Huo ◽  
Yaqiong Zhang ◽  
Caixia Yin
Keyword(s):  
Biomedical Research ◽  
Nucleophilic Addition ◽  
Recent Progress ◽  
Biological Imaging ◽  
Tissue Imaging ◽  
Fluorescence Probes ◽  
Biological Applications ◽  
Convenient Procedure ◽  
Nucleophilic Mechanism

In recent years, aldehyde-appended fluorescence probes have attracted increasing attention. Fluorescent biological imaging includes many modern applications for cell and tissue imaging in biomedical research. Meanwhile, the nucleophilic mechanism is a very simple and convenient procedure for the preparation of aldehyde-sensing probes. This tutorial review focuses on aldehyde-bearing chemosensors based on nucleophilic addition mechanism with biological applications.

scholarly journals Molecular design strategy to alleviate environmental interference on two-photon fluorescence probes

2021 ◽  
Vol 2 (6) ◽  
pp. 100471
Author(s):  
Xingxing Zhang ◽  
Tianbing Ren ◽  
Qianling Zhang ◽  
Ting Zeng ◽  
Zhe Li ◽  
...  
Keyword(s):  
Molecular Design ◽  
Design Strategy ◽  
Fluorescence Probes ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Environmental Interference

scholarly journals Quaternary Alloy Quantum Dots as Fluorescence Probes for Total Acidity Detection of Paper-Based Relics

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1726
Author(s):  
Zhuorui Wang ◽  
Cong Cheng ◽  
Yongjuan Cheng ◽  
Lizhen Zheng ◽  
Daodao Hu
Keyword(s):  
Quantum Dots ◽  
Extraction Method ◽  
Quaternary Alloy ◽  
New Method ◽  
Fluorescence Probes ◽  
Total Acidity ◽  
Weak Acids ◽  
Paper Sample ◽  
Composition And Structure ◽  
Alloy Quantum Dots

Traditionally, the acidity of paper-based relics was determined by an extraction method and using a pH meter. This method could not obtain the total acidity of the paper-based relics because it only detected the concentration of free protons in the aqueous soaking solution. To overcome this defect, a new method for determining the total acidity of paper-based relics has been established by using quaternary alloy quantum dots. The quantum dots, CdZnSeS, modified by p-Aminothiophenol (pATP) were prepared, and their composition and structure were characterized. The fluorescence behavior of prepared quantum dots with acidity was investigated. The following results were obtained. The fluorescence of CdZnSeS-pATP quantum dots could decrease with increases in acidity because pATP dissociated from the surfaces of the quantum dots due to protons or undissociated weak acids. Based on this feature, a method for determining the acidity of paper-based relics was constructed, and this method was used to evaluate the acidity of actual paper-based relics. Obviously, for a given paper sample, since both free protons and bound protons can be determined by this method, the acidity measured by this method is more reasonable than that by pH meter.

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