Lanthanide-doped Fluorescence Probes for NIR-Ⅱ Fluorescence Imaging

2020 ◽  
Vol 41 (12) ◽  
pp. 1460-1478
Author(s):  
Song-tao ZHANG ◽  
◽  
Ying-hui WANG ◽  
Hong-jie ZHANG ◽  
◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Probes

Single-excitation, dual-emission biomass quantum dots: preparation and application for ratiometric fluorescence imaging of coenzyme A in living cells

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9270-9275 ◽  
Author(s):  
Yong Cui ◽  
Rongjun Liu ◽  
Fanggui Ye ◽  
Shulin Zhao
Keyword(s):  
Quantum Dots ◽  
Fluorescence Imaging ◽  
Fluorescence Detection ◽  
Coenzyme A ◽  
Living Cells ◽  
Fluorescence Probes ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Dual Emissions

A distinctive approach is proposed to prepare biomass quantum dots (BQDs) with single-excitation and dual-emissions property. The obtained BQDs have been employed as the fluorescence probes for ratiometric fluorescence detection and imaging of coenzyme A in living cells.

scholarly journals Centimeter-Deep NIR-II Fluorescence Imaging with Nontoxic AIE Probes in Nonhuman Primates

Research ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Zonghai Sheng ◽  
Yaxi Li ◽  
Dehong Hu ◽  
Tianliang Min ◽  
Duyang Gao ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Nonhuman Primates ◽  
Histological Analysis ◽  
Blood Chemistry ◽  
Intravenous Dose ◽  
Vascular Imaging ◽  
Tissue Imaging ◽  
Fluorescence Probes ◽  
Deep Tissue ◽  
Deep Tissue Imaging

Fluorescence probes with aggregation-induced emission (AIE) characteristics are of great importance in biomedical imaging with superior spatial and temporal resolution. However, the lack of toxicity studies and deep tissue imaging in nonhuman primates hinders their clinical translation. Here, we report the blood chemistry and histological analysis in nonhuman primates treated with AIE probes over tenfold of an intravenous dose of clinically used indocyanine green (ICG) during a study period of 36 days to demonstrate AIE probes are nontoxic. Furthermore, through bright and nontoxic AIE probes and fluorescence imaging in the second window (NIR-II, 1,000–1,700 nm), we achieve an unprecedented 1.5-centimeter-deep vascular imaging in nonhuman primates, breaking the current limitation of millimeter-deep NIR-II fluorescence imaging. Our important findings, i.e., nontoxic features of AIE probes and centimeter-deep NIR-II vascular imaging in nonhuman primates, may facilitate successful translation of AIE probes in clinical trials.

scholarly journals Improvement in Tracing Quantum Dot-Conjugated Nanospheres forIn VivoImaging by Eliminating Food Autofluorescence

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Chul-Kyu Park ◽  
Hoonsung Cho
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Signal To Noise Ratio ◽  
Tissue Imaging ◽  
Fluorescence Probes ◽  
Infrared Range ◽  
Long Term Stability ◽  
Magnetic Nanospheres ◽  
Deep Tissue Imaging

Fluorescence imaging using fluorescent probes has demonstrated long-term stability and brightness suitable forin vivodeep-tissue imaging, but it also allows intense background fluorescence associated with food in the near-infrared (IR) range. We investigated effects of changing rodent diet on food autofluorescence, in the presence of quantum dots-conjugated magnetic nanospheres (QD-MNSs). Replacement of a regular rodent diet with a purified diet has great improvement in removing autofluorescence in the near-infrared range ideal forin vivofluorescence imaging. By feeding a purified diet for eliminating ingredients impairing desirable fluorescence signals in the near-IR range, food autofluorescence was clearly eliminated and fluorescence probes, QD-MNSs, introduced by i.v. injection were effectively traced in a mouse by a distinctive signal-to-noise ratio.

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

Ubiquinone-BODIPY nanoparticles for tumor redox-responsive fluorescence imaging and photodynamic activity

2021 ◽  
Author(s):  
Byunghee Hwang ◽  
Tae-Il Kim ◽  
Hyunjin Kim ◽  
Sungjin Jeon ◽  
Yongdoo Choi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Selective Activation ◽  
Turn On ◽  
Intracellular Environment ◽  
Redox Responsive ◽  
Photodynamic Activity

A ubiquinone-BODIPY photosensitizer self-assembles into nanoparticles (PS-Q-NPs) and undergoes selective activation within the highly reductive intracellular environment of tumors, resulting in “turn-on” fluorescence and photosensitizing activities.

Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

Confocal Fluorescence Imaging of Photosensitised DNA Denaturation in Cell Nuclei

2005 ◽  
Author(s):  
Tytus Bernas ◽  
Elikplimi K. Asem ◽  
J. Paul Robinson ◽  
Peter R. Cook ◽  
Jurek W. Dobrucki
Keyword(s):  
Fluorescence Imaging ◽  
Dna Denaturation ◽  
Cell Nuclei ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging
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