scholarly journals Transcriptional regulation of rat alpha 1-acid glycoprotein gene by phenobarbital.

1994 ◽  
Vol 269 (44) ◽  
pp. 27175-27178
Author(s):  
T Fournier ◽  
N Mejdoubi ◽  
C Lapoumeroulie ◽  
J Hamelin ◽  
J Elion ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Acid Glycoprotein ◽  
Glycoprotein Gene

The cytokine response element of the rat alpha 1-acid glycoprotein gene is a complex of several interacting regulatory sequences

1990 ◽  
Vol 10 (8) ◽  
pp. 3965-3978
Author(s):  
K A Won ◽  
H Baumann
Keyword(s):  
Transcriptional Start Site ◽  
Regulatory Region ◽  
Simian Virus 40 ◽  
Hepatoma Cells ◽  
Cytokine Response ◽  
Regulatory Function ◽  
Regulatory Sequences ◽  
Acid Glycoprotein ◽  
Glycoprotein Gene ◽  
Response Region

Expression of the rat alpha 1-acid glycoprotein gene is stimulated by interleukin-1 (IL-1) and interleukin-6 (IL-6) and is synergistically enhanced by the combination of the two. The distal regulatory element (DRE), a 142-base-pair (bp) sequence located 5 kilobase pairs upstream of the transcriptional start site, appears to be crucial for this cytokine response. The cytokine-specific regulatory sequences within the DRE have been identified by inserting individual DRE subregions, selected combinations of these, or a few linker mutated fragments into a plasmid containing an enhancerless simian virus 40 promoter linked to the chloramphenicol acetyltransferase gene. The regulatory activity was determined in transiently transfected human and rat hepatoma cells. The IL-1 response region was confined to the 5'-most 62 bp of the DRE, and its function seemed to depend on at least two separate components. The same region was also responsive to phorbol ester treatment. The IL-6 regulatory function was dependent on a 54-bp sequence located within the 3' half of the DRE. When the IL-1 response region was recombined with the IL-6 regulatory region of the DRE or with IL-6 response elements of other plasma protein genes, a strong cooperative action by IL-1 and IL-6 was achieved. The functional DRE sequences were recognized by nuclear proteins extracted from rat liver and hepatoma cells. However, no cytokine-inducible binding activity was detectable, which suggests that transcriptional regulation through the DRE might be controlled by posttranslational modification of constitutively bound trans-acting factors.

scholarly journals AGP/EBP(LAP) expressed in rat hepatoma cells interacts with multiple promoter sites and is necessary for maximal glucocorticoid induction of the rat alpha-1 acid glycoprotein gene.

1991 ◽  
Vol 11 (10) ◽  
pp. 4959-4965 ◽  
Author(s):  
P Williams ◽  
T Ratajczak ◽  
S C Lee ◽  
G M Ringold
Keyword(s):  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Acid Glycoprotein ◽  
Glycoprotein Gene ◽  
Recognition Sites ◽  
Glucocorticoid Response ◽  
Glucocorticoid Induction ◽  
Multiple Promoter ◽  
Rat Hepatoma Cell Line ◽  
Rat Hepatoma

Transcription of the rat alpha 1-acid glycoprotein (AGP) gene is induced by glucocorticoids. In addition to the glucocorticoid response element which maps to bases -120 to -107, sequences located between bases -106 to -42 have been shown to be necessary for hormone induction. We have previously identified multiple sites of C/EBP interaction with the AGP promoter in the region -106 to -64. In this study, we purify and identify a C/EBP family member, AGP/EBP(LAP), present in the rat hepatoma cell line HTC (JZ.1) which also binds to the C/EBP recognition sites in this region. Mutations in the recognition sites that prevent binding are analyzed, and the results suggest a positive as well as a possible inhibitory role for AGP/EBP(LAP) in the glucocorticoid induction of the gene in HTC (JZ.1) cells.

Induction of liver alpha-1 acid glycoprotein gene expression involves both positive and negative transcription factors

1993 ◽  
Vol 13 (1) ◽  
pp. 432-442
Author(s):  
Y M Lee ◽  
W H Tsai ◽  
M Y Lai ◽  
D S Chen ◽  
S C Lee
Keyword(s):  
Acute Phase ◽  
Regulatory Element ◽  
Site Directed Mutagenesis ◽  
Phase Reaction ◽  
Acid Glycoprotein ◽  
Glycoprotein Gene ◽  
Response Data ◽  
Heat Stable ◽  
Dna Binding Factors ◽  
Negative Factors

Expression of the alpha-1 acid glycoprotein (AGP) gene is liver specific and acute phase responsive. Within the 180-bp region of the AGP promoter, at least five cis elements have been found to interact with trans-acting factors. Four of these elements (A, C, D, and E) interacted with AGP/EBP, a liver-enriched transcription factor, as shown by footprinting analysis and by an anti-AGP/EBP antibody-induced supershift in a gel retardation assay. Modification of these sites by site-directed mutagenesis coupled with transfection analysis indicated that AGP/EBP binding to all of these sites resulted in positive regulation of the promoter. Dose-response data suggest that AGP/EBP binding to these sites results in the cooperative activation of the promoter. In contrast, functional assays showed that element B is a negative regulatory element; this element is recognized by heat-stable DNA-binding factors which are found in many cells and tissues. The regulation of these binding proteins was studied in rat liver treated with lipopolysaccharide (LPS), which induced an acute-phase reaction. We found that LPS treatment resulted in a two- to threefold increase in AGP/EBP activity and a severalfold decrease in the activity of factors that bind to element B in the liver. These results indicate that expression of the AGP gene can be regulated by both positive and negative factors and that the modulation of these factors can account for the LPS induction of the AGP gene.

AGP/EBP(LAP) expressed in rat hepatoma cells interacts with multiple promoter sites and is necessary for maximal glucocorticoid induction of the rat alpha-1 acid glycoprotein gene

1991 ◽  
Vol 11 (10) ◽  
pp. 4959-4965
Author(s):  
P Williams ◽  
T Ratajczak ◽  
S C Lee ◽  
G M Ringold
Keyword(s):  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Acid Glycoprotein ◽  
Glycoprotein Gene ◽  
Recognition Sites ◽  
Glucocorticoid Response ◽  
Glucocorticoid Induction ◽  
Multiple Promoter ◽  
Rat Hepatoma Cell Line ◽  
Rat Hepatoma

Transcription of the rat alpha 1-acid glycoprotein (AGP) gene is induced by glucocorticoids. In addition to the glucocorticoid response element which maps to bases -120 to -107, sequences located between bases -106 to -42 have been shown to be necessary for hormone induction. We have previously identified multiple sites of C/EBP interaction with the AGP promoter in the region -106 to -64. In this study, we purify and identify a C/EBP family member, AGP/EBP(LAP), present in the rat hepatoma cell line HTC (JZ.1) which also binds to the C/EBP recognition sites in this region. Mutations in the recognition sites that prevent binding are analyzed, and the results suggest a positive as well as a possible inhibitory role for AGP/EBP(LAP) in the glucocorticoid induction of the gene in HTC (JZ.1) cells.

Structure of the rat alpha 1-acid glycoprotein gene

1985 ◽  
Vol 5 (12) ◽  
pp. 3634-3639
Author(s):  
Y C Liao ◽  
J M Taylor ◽  
J L Vannice ◽  
G A Clawson ◽  
E A Smuckler
Keyword(s):  
Nucleotide Sequence ◽  
Receptor Binding ◽  
Binding Sites ◽  
Southern Blot Analysis ◽  
Tata Box ◽  
Base Pairs ◽  
Acid Glycoprotein ◽  
Glycoprotein Gene ◽  
Base Pair Fragment ◽  
Pair Fragment

The complete nucleotide sequence of the rat alpha 1-acid glycoprotein gene has been determined from an isolated lambda recombinant bacteriophage. Southern blot analysis and DNA sequencing indicate that there is only one gene per genome; it contains six exons and is located within a 3,200-base-pair fragment starting from a TATA box and extending to the polyadenylation signal AATAAA. Transcription starts 37 base pairs upstream from the beginning of the translation codon ATG. The TATA box (TATAAA) lies 26 base pairs upstream from this site. The gene contains several potential glucocorticoid receptor-binding sites, both inside and outside the structural gene.

Sign in / Sign up

Export Citation Format

Share Document