scholarly journals The Conversion of Glutamate Carbon to Fatty Acid Carbon via Citrate

1964 ◽  
Vol 239 (5) ◽  
pp. 1305-1309
Author(s):  
J. Madsen ◽  
S. Abraham ◽  
I.L. Chaikoff
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Carbon

scholarly journals Acetate represents a major product of heptanoate and octanoate β-oxidation in hepatocytes isolated from neonatal piglets

1996 ◽  
Vol 318 (1) ◽  
pp. 235-240 ◽  
Author(s):  
Xi LIN ◽  
Sean H. ADAMS ◽  
Jack ODLE
Keyword(s):  
Fatty Acid ◽  
Organic Acids ◽  
Chain Length ◽  
Ketone Bodies ◽  
Krebs Cycle ◽  
Major Product ◽  
Carbon Accounting ◽  
Minor Amount ◽  
Fatty Acid Carbon ◽  
Carboxyl Carbon

An experiment was conducted to explore the nature of the radiolabel distribution in acid-soluble products (ASPs) resulting from the oxidation of [1-14C]C7:0 or C8:0 by isolated piglet hepatocytes. The differences between odd and even chain-length and the impacts of valproate and malonate upon the rate of β-oxidation and ASP characteristics were tested. A minor amount of fatty acid carboxyl carbon (⩽ 10% of organic acids identified by radio-HPLC) accumulated in ketone bodies regardless of chain-length or inhibitor used. In all cases, acetate represented the major reservoir of carboxyl carbon, accounting for 60–70% of radiolabel in identified organic acids. Cells given [1-14C]C7:0 accumulated 85% more carboxyl carbon in Krebs cycle intermediates when compared with C8:0, while accumulation in acetate was unaffected. The results are consistent with the hypothesis that anaplerosis from odd-carbon fatty acids affects the oxidative fate of fatty acid carbon. The piglet appears unique in that non-ketogenic routes of fatty acid carbon flow (i.e. acetogenesis) predominate in the liver of this species.

scholarly journals Reconstruction of prehistoric pottery use from fatty acid carbon isotope signatures using Bayesian inference

2018 ◽  
Vol 117 ◽  
pp. 31-42 ◽  
Author(s):  
Ricardo Fernandes ◽  
Yvette Eley ◽  
Marek Brabec ◽  
Alexandre Lucquin ◽  
Andrew Millard ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Bayesian Inference ◽  
Carbon Isotope ◽  
Prehistoric Pottery ◽  
Fatty Acid Carbon

scholarly journals Development of a New Strategy for Production of Medium-Chain-Length Polyhydroxyalkanoates by Recombinant Escherichia coli via Inexpensive Non-Fatty Acid Feedstocks

2011 ◽  
Vol 78 (2) ◽  
pp. 519-527 ◽  
Author(s):  
Qin Wang ◽  
Ryan C. Tappel ◽  
Chengjun Zhu ◽  
Christopher T. Nomura
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Chain Length ◽  
Acyl Carrier Protein ◽  
Carbon Sources ◽  
Content Type ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Coa Transferase ◽  
Fatty Acid Carbon

ABSTRACTPseudomonas putidaKT2440 is capable of producing medium-chain-length polyhydroxyalkanoates (MCL-PHAs) when grown on unrelated carbon sources during nutrient limitation. Transcription levels of genes putatively involved in PHA biosynthesis were assessed by quantitative real-time PCR (qRT-PCR) inP. putidagrown on glycerol as a sole carbon source. The results showed that two genes,phaGand the PP0763 gene, were highly upregulated among genes potentially involved in the biosynthesis of MCL-PHAs from unrelated carbon sources. Previous studies have describedphaGas a 3-hydroxyacyl-acyl carrier protein (ACP)-coenzyme A (CoA) transferase, and based on homology, the PP0763 gene was predicted to encode a medium-chain-fatty-acid CoA ligase. High expression levels of these genes during PHA production inP. putidaled to the hypothesis that these two genes are involved in PHA biosynthesis from non-fatty acid carbon sources, such as glucose and glycerol. ThephaGppand PP0763 genes fromP. putidawere cloned and coexpressed with the engineeredPseudomonassp. 61-3 PHA synthase genephaCl(STQK)psin recombinantEscherichia coli. Up to 400 mg liter−1MCL-PHAs was successfully produced from glucose. This study has produced the largest amount of MCL-PHAs reported from non-fatty acid carbon sources in recombinantE. colito date and opens up the possibility of using inexpensive feedstocks to produce MCL-PHA polymers.

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