Reversed-phase liquid chromatography for enrichment of very-long-chain fatty acids and their identification by gas chromatography-mass spectrometry

1989 ◽  
Vol 472 ◽  
pp. 290-295 ◽  
Author(s):  
T. Řezanka ◽  
T. Koza ◽  
R. Kysilka ◽  
I. Víden ◽  
M. Wurst
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Reversed Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Long Chain Fatty Acids ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid ◽  
Long Chain

scholarly journals Identification and Quantification of Sucrose Esters in Various Turkish Tobaccos

2005 ◽  
Vol 21 (8) ◽  
pp. 441-450 ◽  
Author(s):  
N Ashraf-Khorassani ◽  
N Nazem ◽  
LT Taylor ◽  
WM Coleman
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Reversed Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Liquid Chromatography Mass Spectrometry ◽  
Sucrose Esters ◽  
Reversed Phase Liquid Chromatography ◽  
Chromatography Mass Spectrometry ◽  
Molecular Masses ◽  
Phase Liquid

AbstractGas chromatography-mass spectrometry (GC-MS) and reversed-phase liquid chromatography-mass spectrometry (LC-MS) were used to compare and quantify sucrose esters (SE) in five different tobaccos. The concentration of SE varied from 340 to 950 µg/g. In order to quantify the SE in each tobacco, the trimethylsilane (TMS) derivative of each sample was analyzed via GC-MS. It appeared that in the five tobaccos, the intensities of the five chosen SE-derived molecular masses increased in the order m/z 622 < 636 < 678 < 650 < 664 for samples 1-3, but for samples 4 and 5, the m/z 678 and 650 were reversed. In order to qualitatively characterize the various SE chromatographic fractions LC-MS was used. It was determined that the lowest parent SE m/z in which acetyl was attached to glucose was detected at m/z 566 (10 carbons), and the highest m/z was found at 692 (19 carbons). Some m/z values showed more than one peak which indicated the presence of isomers. All samples showed the presence of at least five different SE peaks wherein acetyl was attached to fructose (i.e. m/z 205). Also, acyl unsaturation was observed in selected fractions.

Phthalate interference in gas-chromatographic determination of long-chain fatty acids in plasma.

1979 ◽  
Vol 25 (2) ◽  
pp. 308-310 ◽  
Author(s):  
C Geisler ◽  
A R Swanson ◽  
L Zieve ◽  
M W Anders
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Accurate Determination ◽  
Chromatographic Determination ◽  
Gas Chromatography Mass Spectrometry ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

Abstract Phthalates leached from plastic tubing or other plastic devices may interfere with gas-chromatographic determination of long-chain fatty acids in plasma. In the case of hospitalized patients who have received infusions or had blood drawn through plastic tubing, gas chromatography/mass spectrometry may be required for accurate determination of such fatty acids.

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