Gas chromatography/mass spectrometry analysis of very long chain fatty acids, docosahexaenoic acid, phytanic acid and plasmalogen for the screening of peroxisomal disorders

2003 ◽  
Vol 25 (7) ◽  
pp. 481-487 ◽  
Author(s):  
Yasuhiko Takemoto ◽  
Yasuyuki Suzuki ◽  
Ryoko Horibe ◽  
Nobuyuki Shimozawa ◽  
Ronald J.A. Wanders ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Phytanic Acid ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Long Chain Fatty Acids ◽  
Peroxisomal Disorders ◽  
Spectrometry Analysis ◽  
Long Chain

Alternative method for gas chromatography-mass spectrometry analysis of short-chain fatty acids in faecal samples

2012 ◽  
Vol 35 (15) ◽  
pp. 1906-1913 ◽  
Author(s):  
Rocio García-Villalba ◽  
Juan A. Giménez-Bastida ◽  
Maria T. García-Conesa ◽  
Francisco A. Tomás-Barberán ◽  
Juan Carlos Espín ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Alternative Method ◽  
Faecal Samples

Production of new extracellular glycolipids by a strain ofCellulomonas cellulans(Oerskovia xanthineolytica) and their structural characterization

1998 ◽  
Vol 44 (3) ◽  
pp. 238-243 ◽  
Author(s):  
S Arino ◽  
R Marchal ◽  
J -P Vandecasteele
Keyword(s):  
Mass Spectrometry ◽  
Fatty Acids ◽  
Gas Chromatography ◽  
Cell Wall ◽  
Culture Medium ◽  
Soil Samples ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectrometry Analysis ◽  
Hydrolysis Of

Glycolipid-producing bacteria were isolated from soil samples. One of the strains, identified as Cellulomonas cellulans (Oerskovia xanthineolytica), was found to produce significant amounts of unusual extracellular glycolipids, which were shown to be composed of at least 11 individual compounds. Hydrolysis of the glycolipid mixture and gas chromatography - mass spectrometry analysis revealed the presence of fatty acids and hydroxy fatty acids ranging from C10to C18, 16 of which were identified. The glycidic moiety consisted of glucose, rhamnose, and ribose. The same sugars were found to be present in the cell wall of Cellulomonas cellulans, which also contained polar lipids including glycolipids. During strain cultivation, glycolipid excretion was stimulated when nitrogen was exhausted from the culture medium. In these conditions, the production in fermenters on glycerol, expressed in glucose equivalents, reached 8.9 g/L. Cell hydrophobicity, which rose to 95% during the growth phase, decreased to 50% during the production phase. The overall results show that the bacterial cell wall is involved in the synthesis of these new extracellular glycolipids.Key words: glycolipid, excretion, Cellulomonas cellulans, Oerskovia xanthineolytica, cell wall.

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