Determination of indole-3-acetic acid and abscisic acid in Japanese chestnuts by column chromatography on sephadex G-10 and reversed-phase high-performance liquid chromatography

1984 ◽  
Vol 287 ◽  
pp. 121-127 ◽  
Author(s):  
Nobuyuki Kozukue ◽  
Etsuko Kozukue ◽  
Lung-Ming Tsay ◽  
Minoru Sawano ◽  
Susumu Mizuno
Keyword(s):  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Abscisic Acid ◽  
Liquid Chromatography ◽  
Column Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Indole 3 Acetic Acid

Simultaneous determination of acidic 3,4-dihydroxyphenylalanine metabolites and 5-hydroxyindole-3-acetic acid in urine by high-performance liquid chromatography

1990 ◽  
Vol 36 (10) ◽  
pp. 1834-1837 ◽  
Author(s):  
A E Stroomer ◽  
H Overmars ◽  
N G Abeling ◽  
A H van Gennip
Keyword(s):  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Reversed Phase ◽  
Fluorescence Detector ◽  
Gas Chromatographic Method ◽  
High Degree

Abstract We describe a simple and rapid quantitative method for the simultaneous determination of 3,4-dihydroxyphenylalanine acid metabolites and 5-hydroxyindole-3-acetic acid. After solvent extraction from acidified urine, the acids are analyzed by reversed-phase high-performance liquid chromatography. For detection and quantification, we used a fluorescence detector in combination with an amperometric detector to obtain a high degree of specificity. Sample preparation and chromatographic analysis can be completed within an hour. Results by the method correlate well with those by a previously used gas-chromatographic method, but the new method is faster and avoids the need for derivatization.

scholarly journals Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid

2021 ◽  
Vol 13 (13) ◽  
pp. 6998
Author(s):  
Beibei Qi ◽  
Chao Wu ◽  
Huiling Liang ◽  
Kehui Cui ◽  
Shah Fahad ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Abscisic Acid ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Xylem Sap ◽  
Column Temperature ◽  
Relative Standard ◽  
Indole 3 Acetic Acid

Liquid-liquid extraction and solid phase extraction followed by high-performance liquid chromatography (HPLC) connected with ultraviolet (UV) detection were used for the determination of phytohormones. The parameters influencing the performance of the HPLC-UV method, including composition of the mobile phase for gradient elution, column temperature, flow rate, and detection wavelength, were optimized. This method can simultaneously determine 11 phytohormones, including nine cytokinins, indole-3-acetic acid, and abscisic acid. The limit of detection of this method is 0.22 to 1.1 µg L−1, and the coefficient factors of linear regression are >0.998. The recoveries of the target phytohormones ranged between 62.1~109.4%, and the relative standard deviations were <10%. This method is suitable for determining phytohormones, especially cytokinins, in young panicles, roots, and xylem sap of rice plants.

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