scholarly journals Concurrent profiling of indole-3-acetic acid, abscisic acid, and cytokinins and structurally related purines by high-performance-liquid-chromatography tandem electrospray mass spectrometry

Plant Methods ◽  
2012 ◽  
Vol 8 (1) ◽  
pp. 42 ◽  
Author(s):  
Scott C Farrow ◽  
RJ Neil Emery
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Abscisic Acid ◽  
Liquid Chromatography ◽  
High Performance ◽  
Electrospray Mass Spectrometry ◽  
Indole 3 Acetic Acid

scholarly journals Identification of New Metabolites of Bacterial Transformation of Indole by Gas Chromatography-Mass Spectrometry and High Performance Liquid Chromatography

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Pankaj Kumar Arora ◽  
Hanhong Bae
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
High Performance ◽  
Glyoxylic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Indole 3 Acetic Acid

Arthrobactersp. SPG transformed indole completely in the presence of an additional carbon source. High performance liquid chromatography and gas chromatography-mass spectrometry detected indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde as biotransformation products. This is the first report of the formation of indole-3-acetic acid, indole-3-glyoxylic acid, and indole-3-aldehyde from indole by any bacterium.

scholarly journals Optimized High-Performance Liquid Chromatography Method for Determining Nine Cytokinins, Indole-3-acetic Acid and Abscisic Acid

2021 ◽  
Vol 13 (13) ◽  
pp. 6998
Author(s):  
Beibei Qi ◽  
Chao Wu ◽  
Huiling Liang ◽  
Kehui Cui ◽  
Shah Fahad ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Acetic Acid ◽  
Abscisic Acid ◽  
Liquid Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Xylem Sap ◽  
Column Temperature ◽  
Relative Standard ◽  
Indole 3 Acetic Acid

Liquid-liquid extraction and solid phase extraction followed by high-performance liquid chromatography (HPLC) connected with ultraviolet (UV) detection were used for the determination of phytohormones. The parameters influencing the performance of the HPLC-UV method, including composition of the mobile phase for gradient elution, column temperature, flow rate, and detection wavelength, were optimized. This method can simultaneously determine 11 phytohormones, including nine cytokinins, indole-3-acetic acid, and abscisic acid. The limit of detection of this method is 0.22 to 1.1 µg L−1, and the coefficient factors of linear regression are >0.998. The recoveries of the target phytohormones ranged between 62.1~109.4%, and the relative standard deviations were <10%. This method is suitable for determining phytohormones, especially cytokinins, in young panicles, roots, and xylem sap of rice plants.

Sign in / Sign up

Export Citation Format

Share Document