Ethanol-sodium chloride-phosphate mobile phase for size-exclusion chromatography of poly(ethylene glycol) modified proteins

1997 ◽  
Vol 763 (1-2) ◽  
pp. 337-344 ◽  
Author(s):  
Joseph J. Ratto ◽  
Sheila R. O'Conner ◽  
Adrian R. Distler ◽  
Gay-May Wu ◽  
David Hummel ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Ethylene Glycol ◽  
Size Exclusion Chromatography ◽  
Mobile Phase ◽  
Size Exclusion ◽  
Poly Ethylene Glycol ◽  
Exclusion Chromatography ◽  
Modified Proteins ◽  
Poly Ethylene

Synthesis and Application of New Photocrosslinkers for Poly(ethylene glycol)

2012 ◽  
Vol 65 (2) ◽  
pp. 193 ◽  
Author(s):  
Hakim Mehenni ◽  
Vincent Pourcelle ◽  
Jean-François Gohy ◽  
Jacqueline Marchand-Brynaert
Keyword(s):  
Polyethylene Glycol ◽  
Ethylene Glycol ◽  
Functional Polymers ◽  
Size Exclusion ◽  
Poly Ethylene Glycol ◽  
Polymer Backbone ◽  
Exclusion Chromatography ◽  
Exogenous Agents ◽  
Commercial Sources ◽  
Poly Ethylene

Photocrosslinking of polyethylene glycol (PEG) using exogenous agents is a convenient way to produce branched PEG from commercial sources thus avoiding the tricky synthesis of new reactive and functional polymers. In this study, we synthesized two series of new photocrosslinkers, i.e. bis-fluorophenyl azide and bis-trifluoromethyl diazirine, which under soft UV-irradiation produce reactive species (i.e. nitrene and carbene respectively) that insert into the C–H bond of the polymer backbone, building new bridges between macromolecular chains. These photocrosslinkers are different in terms of behaviour under irradiation and affinity for the target substrate (i.e. PEG). Thus, practical conditions for photocrosslinking of a 10-kDa PEG were studied and followed by NMR and size-exclusion chromatography. In particular, we investigated irradiation in bulk or in solvent, at different irradiation times, with several concentrations of PEG and photolinkers. Finally, we were able to design a procedure to obtain soluble crosslinked PEGs of 300 kDa.

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