Optimisation and routine use of generic ultra-high flow-rate liquid chromatography with mass spectrometric detection for the direct on-line analysis of pharmaceuticals in plasma

1998 ◽  
Vol 828 (1-2) ◽  
pp. 199-207 ◽  
Author(s):  
J Ayrton ◽  
G.J Dear ◽  
W.J Leavens ◽  
D.N Mallett ◽  
R.S Plumb
Keyword(s):  
Liquid Chromatography ◽  
Flow Rate ◽  
High Flow ◽  
Mass Spectrometric ◽  
High Flow Rate ◽  
Mass Spectrometric Detection ◽  
On Line ◽  
Line Analysis

Performance Evaluation of Cascade Filtration with High Flow Rate Recirculating Plasma on the Secondary Filter

1987 ◽  
Vol 10 (2) ◽  
pp. 121-128 ◽  
Author(s):  
G. Busnach ◽  
A. Dal Col ◽  
M.L. Perrino ◽  
B. Brando ◽  
C. Brunati ◽  
...  
Keyword(s):  
Small Molecules ◽  
Flow Rate ◽  
High Flow ◽  
High Flow Rate ◽  
Fractionation Process ◽  
Dead End ◽  
On Line ◽  
Primary Separation ◽  
G Ratio ◽  
Additional Loss

Cascade filtration (CF) has been performed in 67 on-line procedures in 9 normolipidemic patients with paraproteinemic disorders. A modified dead-end technique has been employed, with high flow rate recirculating plasma on the plasma fractionation filter (QD recycled CF), and an albumin-rich, globulin-poor filtrate was reinfused into the patient. Postprocedure recoveries were 81 ± 15% for albumin, 55 ± 23% for IgM and 48% for cryocrit, with an increase in A/G ratio from 1.8 to 2.1. An improvement was observed also in antiatherogenic/atherogenic lipoproteins ratio, suggesting a possible use of this technique in the treatment of familial hypercholesterolemia. Plasma primary separation was obtained by centrifugation or by filtration, and no significant differences were observed on subsequent protein fractionation process. An albumin priming of the plasmafractionation circuit accounted for an additional 13% saving in postprocedure level. Different surface area secondary filters have been employed: with larger surfaces, larger volumes were processed without any increase in the waste volume and with reduced need for washouts, but with an additional loss of small molecules possibly due to entrapping onto the membrane.

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