Chemotaxis of polymorphonuclear leukocytes in whole blood in the ‘sparse-pore’ polycarbonate (Nuclepore) membrane/Boyden chamber assay

1992 ◽  
Vol 149 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Jane E. Rice ◽  
Leon P. Bignold
Keyword(s):  
Whole Blood ◽  
Polymorphonuclear Leukocytes ◽  
Boyden Chamber ◽  
Boyden Chamber Assay ◽  
Nuclepore Membrane

scholarly journals Indole Monoterpenes with Antichemotactic Activity from Psychotria myriantha: Chemotaxonomic Significance

2006 ◽  
Vol 1 (12) ◽  
pp. 1934578X0600101
Author(s):  
Cláudia A. Simões-Pires ◽  
Fabianne M. Farias ◽  
Andrew Marston ◽  
Emerson F. Queiroz ◽  
Célia G. Chaves ◽  
...  
Keyword(s):  
Natural Product ◽  
Crude Extract ◽  
Polymorphonuclear Leukocytes ◽  
The Other ◽  
Boyden Chamber ◽  
Preparative Hplc ◽  
Spectroscopic Methods ◽  
Active Compounds ◽  
Boyden Chamber Assay ◽  
Aerial Parts

The alkaloid extract of the aerial parts of Psychotria myriantha (Rubiaceae) displayed antichemotactic activity on polymorphonuclear leukocytes (PMN) assessed by the Boyden chamber assay. On analysis of the crude extract by LC/APCI/MS and LC/UV/DAD, two major constituents could be detected. In order to rapidly identify the active compounds, a microfractionation was conducted during LC/UV/DAD analysis. By this means, both the collected compounds could be assayed separately in the Boyden chamber and were shown to inhibit PMN chemotaxis. Their isolation was performed by semi-preparative HPLC and their structures elucidated by classical spectroscopic methods, including UV, NMR, MS and HRMS. Both compounds showed characteristics of monoterpene indole glucoside alkaloids; one of them was identified as strictosidinic acid and the other was a new natural product, myrianthosine. The antichemotactic activity of the compounds may be related to an antiacute inflammation activity.

Effect of Some Anti-Inflammatory Drugs on Human Neutrophil Chemotaxis

1994 ◽  
Vol 22 (2) ◽  
pp. 100-106 ◽  
Author(s):  
G Hasçelik ◽  
B ŞLener ◽  
Z Hasçelik
Keyword(s):  
Acetylsalicylic Acid ◽  
Polymorphonuclear Leukocyte ◽  
Polymorphonuclear Leukocytes ◽  
Diclofenac Sodium ◽  
Tiaprofenic Acid ◽  
Boyden Chamber ◽  
Random Migration ◽  
Leukocyte Chemotaxis ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

The effects of piroxicam, tenoxicam, diclofenac sodium, acetylsalicylic acid and tiaprofenic acid on the chemotaxis and random migration of human polymorphonuclear leukocytes were investigated, using zymosan-activated serum as chemo-attractant, with a modified Boyden chamber technique. All five compounds significantly reduced chemotaxis. The random migration of polymorphonuclear leukocytes was inhibited by piroxicam, diclofenac sodium and tiaprofenic acid but not by tenoxicam or acetylsalicylic acid. The inhibitory effect of these non-steroidal anti-inflammatory drugs on polymorphonuclear leukocyte chemotaxis and on random migration was generally dose-dependent. The results suggest that the drugs studied may have a direct effect on polymorphonuclear leukocyte chemotaxis and that this activity may contribute to their anti-inflammatory properties.

Determinants of platelet conjugate formation with polymorphonuclear leukocytes or monocytes in whole blood

2007 ◽  
Vol 98 (12) ◽  
pp. 1276-1284 ◽  
Author(s):  
Simona Costanzo ◽  
Branislav Vohnout ◽  
Licia Iacoviello ◽  
Giovanni de Gaetano ◽  
Benedetta Izzi ◽  
...  
Keyword(s):  
Platelet Count ◽  
Whole Blood ◽  
Polymorphonuclear Leukocytes ◽  
Large Population ◽  
Mixed Cell ◽  
Cd11b Expression ◽  
Glucose Levels ◽  
Primary Platelet ◽  
Collagen Stimulation

SummaryFollowing preliminary in-vitro experiments, platelet-leukocyte conjugates and their determinants were evaluated in citrated whole blood from 349 subjects (209 women, age 16–92 years) randomly recruited from the general population. Platelet activation by ADP/collagen but not leukocyte stimulation by fMLP or LTB4 resulted in formation of platelet conjugates with PMN or monocytes. In the population study, mixed cell conjugates, platelet P-selectin and leukocyte CD11b were measured by flow cytometry both at baseline and after in-vitro stimulation with ADP/collagen. The latter significantly increased platelet conjugates with either PMN or monocytes, platelet P-selectin and leukocyte CD11b expression. Platelet count significantly correlated with platelet-PMN, platelet-monocyte conjugates and P-selectin both at baseline and upon stimulation. In all conditions, both conjugate levels correlated with each other, when adjusted for gender, age and platelet count. Age correlated with platelet-PMN conjugate numbers in basal and stimulated conditions and with basal P-selectin. ADP/collagen stimulation resulted in higher P-selectin and conjugates values in women. Among risk factors, a significant correlation was found between conjugate and glucose levels. In conclusion, the presence and formation in whole blood from a large population of plateletleukocyte conjugates reflects primary platelet – but not leukocyte – activation and varies with gender, age, platelet count and blood glucose.

Active Motion of Polymorphonuclear Leukocytes in Response to Chemoattractant in a Micropipette

1993 ◽  
Vol 115 (4B) ◽  
pp. 503-509 ◽  
Author(s):  
B. A. Skierczynski ◽  
S. Usami ◽  
S. Chien ◽  
R. Skalak
Keyword(s):  
Concentration Gradient ◽  
Polymorphonuclear Leukocytes ◽  
Initial Conditions ◽  
Front Face ◽  
Boyden Chamber ◽  
Internal Source ◽  
Exact Nature ◽  
Active Motion ◽  
Cell Locomotion ◽  
Sequence Of Events

A novel experimental method of producing and observing the active motion of polymorphonuclear leukocytes (PMNs) using a micropipette technique has been recently developed (Usami et al., 1992). The present paper develops a quantitative theory for the chemoattractant gradients and cell locomotion observed in these experiments. In previous experimental methods (e.g., the Boyden chamber, the Zygmond chamber and the Dunn chamber) for study chemotaxis of leukocytes, fibroblasts, and PMNs, the exact nature of the concentration gradient of the chemoattractant is unknown. The cells may themselves modify the local gradient of the chemoattractant. In experiments using the micropipette, an internal source of chemoattractant provides well-defined boundary and initial conditions which allow the computation of the chemoattractant concentration gradient during the active locomotion of the PMNs. Since the cell completely fills the pipette lumen, convection is limited to the motion of the cells themselves. In coordinates moving with cell, it is assumed that diffusion is the only mechanism of mass transport of the chemoattractant (fMLP). Computations of the fMLP concentration during locomotion of the cell were carried out for a range of rates of fMLP binding by the receptors expressed on the front face of the cell membrane. The results show that the front face of the cell is subjected to increasing fMLP concentration during the cell motion. The sequence of events involve receptor binding of fMLP, signal transduction, polymerization of the cell cytoskeleton at the membrane of the front face, spatially dependent adhesion to the pipette wall, and localized contraction of the cytoskeleton. This sequence of events leads to the steady locomotion of the leukocytes in the micropipette. The computation of the distribution of the fMLP concentration during cell locomotion with constant velocity in micropipette experiments shows that the cell is exposed to increasing concentration of fMLP. This suggests that chemotaxis maybe induced by temporal gradient of an attractant.

Comparison of circulating phagocyte oxidative activity measured by chemiluminescence in whole blood and isolated polymorphonuclear leukocytes

1996 ◽  
Vol 253 (1-2) ◽  
pp. 145-157 ◽  
Author(s):  
Steffi Kopprasch ◽  
J. Graessler ◽  
M. Kohl ◽  
S. Bergmann ◽  
H.-E. Schröder
Keyword(s):  
Whole Blood ◽  
Polymorphonuclear Leukocytes ◽  
Oxidative Activity
Sign in / Sign up

Export Citation Format

Share Document