scholarly journals A rapid, simple, and sensitive procedure for the determination of free fatty acids in plasma using glass capillary column gas-liquid chromatography.

1981 ◽  
Vol 22 (5) ◽  
pp. 852-858
Author(s):  
K Y Tserng ◽  
R M Kliegman ◽  
E L Miettinen ◽  
S C Kalhan
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Free Fatty Acids ◽  
Capillary Column ◽  
Glass Capillary Column ◽  
Gas Liquid Chromatography ◽  
Glass Capillary

Identification and metabolic implication of 1-deoxyglucose (1,5-anhydroglucitol) in human plasma.

1982 ◽  
Vol 28 (6) ◽  
pp. 1283-1286 ◽  
Author(s):  
S Yoshioka ◽  
S Saitoh ◽  
T Fujisawa ◽  
A Fujimori ◽  
O Takatani ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Human Plasma ◽  
Capillary Column ◽  
Physiological Role ◽  
Glass Capillary Column ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Glass Capillary ◽  
Liquid Chromatograph

Abstract 1-Deoxyglucose (1,5-anhydroglucitol), a metabolite related to diabetes mellitus, was identified in human plasma by gas-liquid chromatography and by gas-liquid chromatography/mass spectrometry. Plasma polyols were accurately determined with a gas-liquid chromatograph equipped with an all-glass capillary column. The plasma content of 1-deoxyglucose in healthy persons varies with age. Although the precise physiological role of 1-deoxyglucose remains obscure, the method described here for determining the minor polyol components of plasma, as well as the findings of 1-deoxyglucose in the plasma of healthy subjects, may be useful for investigating the metabolic roles of 1-deoxyglucose.

A method for the quantitative determination of individual free fatty acids in milk by ion exchange resin adsorption and gas-liquid chromatography

1983 ◽  
Vol 50 (3) ◽  
pp. 321-329 ◽  
Author(s):  
Eric C. Needs ◽  
Graeme D. Ford ◽  
A. Jane Owen ◽  
Brian Tuckley ◽  
Malcolm Anderson
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Free Fatty Acids ◽  
Exchange Resin ◽  
Ion Exchange Resin ◽  
Gas Liquid Chromatography ◽  
Internal Standards ◽  
Resin Adsorption ◽  
The Individual

SummaryA quantitative method for rapid routine analysis of individual free fatty acids (FFA) in milk was developed. Lipid was extracted from milk in ether and FFA were recovered by shaking the extract with anion exchange resin Amberlyst 26. The resin-bound FFA were methylated directly and the individual acids quantified, using internal standards, by gas-liquid chromatography. The properties of the resin were measured. The validity of the method was established by extraction of FFA mixtures and milk. Individual acids were, on average, found to be within 6% of the actual concentration present in the mixture. An average coefficient of variation of 4·3% was achieved for the major individual fatty acids on repeated extraction of a single milk sample.

Resolution of alkenylacylglycerol moieties of natural glycerophospholipids by gas–liquid chromatography on polar capillary columns

1984 ◽  
Vol 62 (6) ◽  
pp. 352-362 ◽  
Author(s):  
J. J. Myher ◽  
A. Kuksis
Keyword(s):  
Liquid Chromatography ◽  
Carbon Number ◽  
Glass Capillary Column ◽  
Gas Liquid Chromatography ◽  
Glass Capillary ◽  
Practical Procedure ◽  
Flame Ionization ◽  
Column Wall ◽  
Layer Chromatography

A sensitive and practical procedure has been developed for the determination of the sn-1-alk-1-enyl-2-acylglycerol moieties of natural glycerophospholipids using capillary gas–liquid chromatography with flame ionization detection. The method gives excellent resolution and quantitative estimates for molecular species according to carbon number and degree of unsaturation. The diradylglycerols are released from the glycerophospholipids by hydrolysis with phospholipase C and the alk-1-enylacylglycerols are resolved by thin-layer chromatography. Either the trimethylsilyl or the tertiary-butyldimethylsilyl ethers are then resolved at 250 °C by gas–liquid chromatography on a glass capillary column wall-coated with a polar cyanopropylsiloxane polymer using hydrogen as the carrier gas. The method has been applied to the analysis of the alk-1-enylacyl- and diacyl-glycerol moieties of the phosphatidylethanolamines of rat heart and kidney.

Identification of low-boiling fraction of pyrolysis oil

1983 ◽  
Vol 48 (1) ◽  
pp. 60-70
Author(s):  
Jan Novrocík ◽  
Marta Novrocíková
Keyword(s):  
Capillary Column ◽  
Glass Capillary Column ◽  
Gas Liquid Chromatography ◽  
Pyrolysis Oil ◽  
Glass Capillary ◽  
Identification Methods ◽  
Oil Fraction ◽  
Continuous Rectification ◽  
Determination Of Parameters

Composition of the low-boiling fraction of the pyrolysis oil obtained from continuous rectification has been determined by combination of capillary gas-liquid chromatography with other identification methods (catalytic hydrogenation, polymerization). In this way components have been identified which form overall 86.0 per cent (m/m) of the low-boiling fraction. The said pyrolysis oil fraction has been found to contain almost 50 per cent (m/m) of unsaturated components able of polymerization, especially methylindenes, methyl-, ethyl- and dimethylvinylbenzenes, divinylbenzenes and 1,2-dihydronaphthalene. Elution behaviour of all the identified isomeric methylindenes, divinylbenzenes and 1,2-dihydronaphthalene has been evaluated by determination of parameters of the equation Ist.phase (2) = k . Ist.phase(1) + q. The Kovats elution indices of all the identified aromatic hydrocarbons have been determined with the use of a glass capillary column wetted with Carbowax 20M at 80 °C.

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